<?xml version='1.0' encoding='utf-8'?>
<emdEntry accessCode="4523" version="1.9.6" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://github.com/emdb-empiar/emdb-schemas/blob/master/v1/v1_9/emdb_v1_9_6.xsd">
    <admin>
        <lastUpdate>2021-11-22</lastUpdate>
    </admin>
    <deposition>
        <status>OBS</status>
        <depositionDate>2019-01-02</depositionDate>
        <depositionSite>PDBe</depositionSite>
        <processingSite>PDBe</processingSite>
        <headerReleaseDate>2021-12-01</headerReleaseDate>
        <obsoletedDate>2021-12-01</obsoletedDate>
        <title>HPBV HtCP</title>
        <authors>Caston JR, Ortega-Esteban A, Mata CP</authors>
        <fittedPDBEntryIdList>
            <fittedPDBEntryId>6qdp</fittedPDBEntryId>
        </fittedPDBEntryIdList>
        <primaryReference published="false">
            <journalArticle>
                <authors>Caston JR, Ortega-Esteban A, Mata CP</authors>
                <articleTitle>HPBV CP</articleTitle>
                <journal>To Be Published</journal>
                <externalReference type="csd">0353</externalReference>
            </journalArticle>
        </primaryReference>
    </deposition>
    <map>
        <file format="CCP4" sizeKb="256001" type="map">emd_4523.map.gz</file>
        <dataType>Image stored as Reals</dataType>
        <dimensions>
            <numRows>400</numRows>
            <numColumns>400</numColumns>
            <numSections>400</numSections>
        </dimensions>
        <origin>
            <originRow>-200.0</originRow>
            <originCol>-200.0</originCol>
            <originSec>-200.0</originSec>
        </origin>
        <limit>
            <limitRow>199.0</limitRow>
            <limitCol>199.0</limitCol>
            <limitSec>199.0</limitSec>
        </limit>
        <spacing>
            <spacingRow>400</spacingRow>
            <spacingCol>400</spacingCol>
            <spacingSec>400</spacingSec>
        </spacing>
        <cell>
            <cellA units="A">426.00003</cellA>
            <cellB units="A">426.00003</cellB>
            <cellC units="A">426.00003</cellC>
            <cellAlpha units="degrees">90.0</cellAlpha>
            <cellBeta units="degrees">90.0</cellBeta>
            <cellGamma units="degrees">90.0</cellGamma>
        </cell>
        <axisOrder>
            <axisOrderFast>X</axisOrderFast>
            <axisOrderMedium>Y</axisOrderMedium>
            <axisOrderSlow>Z</axisOrderSlow>
        </axisOrder>
        <statistics>
            <minimum>-0.2766266</minimum>
            <maximum>0.5237844</maximum>
            <average>0.0018516082</average>
            <std>0.041539915</std>
        </statistics>
        <spaceGroupNumber>1</spaceGroupNumber>
        <details />
        <pixelSpacing>
            <pixelX units="A">1.065</pixelX>
            <pixelY units="A">1.065</pixelY>
            <pixelZ units="A">1.065</pixelZ>
        </pixelSpacing>
        <contourLevel source="author">0.1</contourLevel>
        <annotationDetails>HPBV HtCP VLP</annotationDetails>
    </map>
    <sample>
        <numComponents>2</numComponents>
        <name>Human picobirnavirus</name>
        <sampleComponentList>
            <sampleComponent componentID="1">
                <entry>virus</entry>
                <sciName>Human picobirnavirus</sciName>
                <virus>
                    <sciSpeciesName ncbiTaxId="145856">Human picobirnavirus</sciSpeciesName>
                    <empty>true</empty>
                    <enveloped>false</enveloped>
                    <isolate>STRAIN</isolate>
                    <class>VIRUS-LIKE PARTICLE</class>
                    <engSource>
                        <expSystem ncbiTaxId="469008">Escherichia coli BL21(DE3)</expSystem>
                        <vector>pRSET</vector>
                    </engSource>
                    <shell id="1">
                        <nameElement>Capsid Protein</nameElement>
                        <diameter units="A">380.0</diameter>
                        <tNumber>1</tNumber>
                    </shell>
                </virus>
            </sampleComponent>
            <sampleComponent componentID="2">
                <entry>protein</entry>
                <sciName>Capsid protein precursor</sciName>
                <molWtTheo units="MDa">0.066223703</molWtTheo>
                <details>Chain B: The side chains of residues from aa77 to aa80 were not well solved and substituted by alanines. The side chain of the residue 588aa was not well solved and substituted by an alanine.Chain A: The side chains of residues aa79 and aa80 were not well solved and substituted by alanines.</details>
                <protein>
                    <sciSpeciesName ncbiTaxId="145856">Human picobirnavirus</sciSpeciesName>
                    <numCopies>2</numCopies>
                    <recombinantExpFlag>false</recombinantExpFlag>
                    <engSource>
                        <expSystem ncbiTaxId="469008">Escherichia coli BL21(DE3)</expSystem>
                    </engSource>
                </protein>
            </sampleComponent>
        </sampleComponentList>
    </sample>
    <experiment>
        <vitrification>
            <cryogenName>ETHANE</cryogenName>
            <instrument>LEICA EM CPC</instrument>
        </vitrification>
        <imaging>
            <electronSource>FIELD EMISSION GUN</electronSource>
            <electronDose units="e/A**2">1.56</electronDose>
            <imagingMode>BRIGHT FIELD</imagingMode>
            <nominalDefocusMin units="nm">800.0</nominalDefocusMin>
            <nominalDefocusMax units="nm">3500.0</nominalDefocusMax>
            <illuminationMode>FLOOD BEAM</illuminationMode>
            <detector>FEI FALCON III (4k x 4k)</detector>
            <nominalCs units="mm">2.7</nominalCs>
            <microscope>FEI TITAN KRIOS</microscope>
            <specimenHolderModel>OTHER</specimenHolderModel>
            <acceleratingVoltage units="kV">300</acceleratingVoltage>
            <nominalMagnification>50000.0</nominalMagnification>
        </imaging>
        <imageAcquisition />
        <fitting>
            <pdbEntryIdList />
            <refSpace>REAL</refSpace>
        </fitting>
        <specimenPreparation>
            <specimenState>particle</specimenState>
            <buffer>
                <ph>7.5</ph>
            </buffer>
        </specimenPreparation>
    </experiment>
    <processing>
        <method>singleParticle</method>
        <reconstruction>
            <software>RELION</software>
            <resolutionByAuthor>2.8</resolutionByAuthor>
            <resolutionMethod>FSC 0.143 CUT-OFF</resolutionMethod>
        </reconstruction>
        <singleParticle>
            <appliedSymmetry>I</appliedSymmetry>
            <numProjections>30342</numProjections>
        </singleParticle>
    </processing>
</emdEntry>