2021-11-22 OBS PDBe PDBe PDBe 2019-01-02 2021-12-01 2021-12-01 2021-11-22 Spanish Ministry of Economy and Competitiveness BFU2017-88736-R Spain Caston JR Ortega-Esteban A Mata CP Caston JR Ortega-Esteban A Mata CP To Be Published 0353 EMD-4523 associated EM volume

HPBV HtCP

6qdp FULLOVERLAP Human picobirnavirus Human picobirnavirus 0 1 Human picobirnavirus Escherichia coli BL21(DE3) pRSET Capsid Protein 380.0 1 VIRUS-LIKE PARTICLE STRAIN false true Capsid protein precursor Human picobirnavirus 0.066223703

Chain B: The side chains of residues from aa77 to aa80 were not well solved and substituted by alanines. The side chain of the residue 588aa was not well solved and substituted by an alanine.Chain A: The side chains of residues aa79 and aa80 were not well solved and substituted by alanines.

2 Escherichia coli BL21(DE3) LEVO MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDRWGSMKQNDTKKTTQRRNSKKYSSKTNRGTKRAPRDQEVGTGAQESTR NDVAWYARYPHILEEATRLPFAYPIGQYYDTGYSVASATEWSKYVDTSLTIPGVMCVNFTPTPGESYNKNSPINIAAQNV YTYVRHMNSGHANYEQADLMMYLLAMDSLYIFHSYVRKILAISKLYTPVNKYFPRALLVALGVDPEDVFANQAQWEYFVN MVAYRAGAFAAPASMTYYERHAWMSNGLYVDQDVTRAQIYMFKPTMLWKYENLGTTGTKLVPLMMPKAGDNRKLVDFQVL FNNLVSTMLGDEDFGIMSGDVFKAFGADGLVKLLAVDSTTMTLPTYDPLILAQIHSARAVGAPILETSTLTGFPGRQWQI TQNPDVNNGAIIFHPSFGYDGQDHEELSFRAMCSNMILNLPGEAHSAEMIIEATRLATMFQVKAVPAGDTSKPVLYLPNG FGTEVVNDYTMISVDKATPHDLTIHTFFNNILVPNAKENYVANLELLNNIIQFDWAPQLYLTYGIAQESFGPFAQLNDWT ILTGETLARMHEVCVTSMFDVPQMGFNK singleParticle particle 7.5 50.0 NaCl 25.0 TrisHCl ETHANE LEICA EM CPC FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 70.0 2.7 0.8 0.306 3.5 4.04 50000.0 FEI FALCON III (4k x 4k) INTEGRATING 1.56 1 CTFFIND

Human Picobirnavirus CP model

I 2.8 FSC 0.143 CUT-OFF RELION 2.1 30342 MAXIMUM LIKELIHOOD RELION 2.1 MAXIMUM LIKELIHOOD RELION 2.1 REAL