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<emdEntry accessCode="4509" version="1.9.6" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://github.com/emdb-empiar/emdb-schemas/blob/master/v1/v1_9/emdb_v1_9_6.xsd">
    <admin>
        <lastUpdate>2019-09-11</lastUpdate>
    </admin>
    <deposition>
        <status>OBS</status>
        <depositionDate>2018-12-28</depositionDate>
        <depositionSite>PDBe</depositionSite>
        <processingSite>PDBe</processingSite>
        <headerReleaseDate>2019-08-21</headerReleaseDate>
        <mapReleaseDate>2019-08-21</mapReleaseDate>
        <obsoletedDate>2019-09-11</obsoletedDate>
        <supersededByList>
            <entry>EMD-4510</entry>
        </supersededByList>
        <title>Cryo em structure of the Listeria stressosome</title>
        <authors>Williams AH, Redzej A</authors>
        <primaryReference published="true">
            <journalArticle>
                <authors>Williams AH, Redzej A, Rolhion N, Costa TRD, Rifflet A, Waksman G, Cossart P</authors>
                <articleTitle>The cryo-electron microscopy supramolecular structure of the bacterial stressosome unveils its mechanism of activation.</articleTitle>
                <journal>Nat Commun</journal>
                <volume>10</volume>
                <firstPage>3005</firstPage>
                <lastPage>3005</lastPage>
                <year>2019</year>
                <externalReference type="pubmed">31285450</externalReference>
                <externalReference type="doi">doi:10.1038/s41467-019-10782-0</externalReference>
                <externalReference type="issn">2041-1723</externalReference>
            </journalArticle>
        </primaryReference>
    </deposition>
    <map>
        <file format="CCP4" sizeKb="275685" type="map">emd_4509.map.gz</file>
        <dataType>Image stored as Reals</dataType>
        <dimensions>
            <numRows>410</numRows>
            <numColumns>410</numColumns>
            <numSections>410</numSections>
        </dimensions>
        <origin>
            <originRow>0.0</originRow>
            <originCol>0.0</originCol>
            <originSec>0.0</originSec>
        </origin>
        <limit>
            <limitRow>409.0</limitRow>
            <limitCol>409.0</limitCol>
            <limitSec>409.0</limitSec>
        </limit>
        <spacing>
            <spacingRow>410</spacingRow>
            <spacingCol>410</spacingCol>
            <spacingSec>410</spacingSec>
        </spacing>
        <cell>
            <cellA units="A">168100.0</cellA>
            <cellB units="A">168100.0</cellB>
            <cellC units="A">168100.0</cellC>
            <cellAlpha units="degrees">90.0</cellAlpha>
            <cellBeta units="degrees">90.0</cellBeta>
            <cellGamma units="degrees">90.0</cellGamma>
        </cell>
        <axisOrder>
            <axisOrderFast>X</axisOrderFast>
            <axisOrderMedium>Y</axisOrderMedium>
            <axisOrderSlow>Z</axisOrderSlow>
        </axisOrder>
        <statistics>
            <minimum>-0.045431003</minimum>
            <maximum>0.0974124</maximum>
            <average>0.0004691323</average>
            <std>0.003942631</std>
        </statistics>
        <spaceGroupNumber>1</spaceGroupNumber>
        <details />
        <pixelSpacing>
            <pixelX units="A">410.0</pixelX>
            <pixelY units="A">410.0</pixelY>
            <pixelZ units="A">410.0</pixelZ>
        </pixelSpacing>
        <contourLevel source="author">0.0152</contourLevel>
    </map>
    <sample>
        <numComponents>4</numComponents>
        <name>Listeria Stressosome</name>
        <details>The subcomponents are, RsbR, RsbR and he RsbT.</details>
        <sampleComponentList>
            <sampleComponent componentID="1">
                <entry>protein</entry>
                <sciName>Listeria Stressosome</sciName>
                <molWtExp units="MDa">1.8</molWtExp>
                <details>The subcomponents are, RsbR, RsbR and he RsbT.</details>
                <protein>
                    <sciSpeciesName ncbiTaxId="1639">Listeria monocytogenes</sciSpeciesName>
                    <sciSpeciesStrain>EGD-e</sciSpeciesStrain>
                    <recombinantExpFlag>false</recombinantExpFlag>
                    <engSource>
                        <expSystem ncbiTaxId="562">Escherichia coli</expSystem>
                        <expSystemStrain>Bl21</expSystemStrain>
                    </engSource>
                </protein>
            </sampleComponent>
            <sampleComponent componentID="2">
                <entry>protein</entry>
                <sciName>RsbR</sciName>
                <protein>
                    <sciSpeciesName ncbiTaxId="1639">Listeria monocytogenes</sciSpeciesName>
                    <recombinantExpFlag>false</recombinantExpFlag>
                    <engSource>
                        <expSystem ncbiTaxId="511693">Escherichia coli BL21</expSystem>
                    </engSource>
                </protein>
            </sampleComponent>
            <sampleComponent componentID="3">
                <entry>protein</entry>
                <sciName>RsbS</sciName>
                <protein>
                    <sciSpeciesName ncbiTaxId="1639">Listeria monocytogenes</sciSpeciesName>
                    <recombinantExpFlag>false</recombinantExpFlag>
                    <engSource>
                        <expSystem ncbiTaxId="511693">Escherichia coli BL21</expSystem>
                    </engSource>
                </protein>
            </sampleComponent>
            <sampleComponent componentID="4">
                <entry>protein</entry>
                <sciName>RsbT</sciName>
                <protein>
                    <sciSpeciesName ncbiTaxId="1639">Listeria monocytogenes</sciSpeciesName>
                    <recombinantExpFlag>false</recombinantExpFlag>
                    <engSource>
                        <expSystem ncbiTaxId="511693">Escherichia coli BL21</expSystem>
                    </engSource>
                </protein>
            </sampleComponent>
        </sampleComponentList>
    </sample>
    <experiment>
        <vitrification>
            <cryogenName>OTHER</cryogenName>
            <details />
        </vitrification>
        <imaging>
            <electronSource>OTHER</electronSource>
            <electronDose units="e/A**2">2.25</electronDose>
            <imagingMode>OTHER</imagingMode>
            <illuminationMode>OTHER</illuminationMode>
            <detector>GATAN K2 QUANTUM (4k x 4k)</detector>
            <microscope>FEI TITAN KRIOS</microscope>
            <specimenHolderModel>FEI TITAN KRIOS AUTOGRID HOLDER</specimenHolderModel>
            <acceleratingVoltage units="kV">300</acceleratingVoltage>
        </imaging>
        <imageAcquisition />
        <fitting>
            <pdbEntryIdList />
            <refSpace>REAL</refSpace>
        </fitting>
        <specimenPreparation>
            <specimenState>particle</specimenState>
            <specimenConc units="mg/ml">0.02</specimenConc>
            <buffer>
                <ph>8.5</ph>
            </buffer>
        </specimenPreparation>
    </experiment>
    <processing>
        <method>singleParticle</method>
        <reconstruction>
            <software>RELION</software>
            <resolutionByAuthor>4.4</resolutionByAuthor>
            <resolutionMethod>FSC 0.143 CUT-OFF</resolutionMethod>
        </reconstruction>
        <singleParticle>
            <appliedSymmetry>C2</appliedSymmetry>
            <numProjections>78000</numProjections>
        </singleParticle>
    </processing>
</emdEntry>