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<emdEntry accessCode="4359" version="1.9.6">
    <admin>
        <lastUpdate>2019-03-27</lastUpdate>
    </admin>
    <deposition>
        <status>OBS</status>
        <depositionDate>2018-04-09</depositionDate>
        <depositionSite>PDBe</depositionSite>
        <processingSite>PDBe</processingSite>
        <headerReleaseDate>2018-05-16</headerReleaseDate>
        <obsoletedDate>2019-03-27</obsoletedDate>
        <title>Cryo-EM structure of a Bacillus subtilis 50S-RbgA-GMPPNP intermediate</title>
        <authors>Wilson DN, Wieland M</authors>
        <primaryReference published="false">
            <journalArticle>
                <authors>Wilson DN, Wieland M, Pausch P, Klaus T, Freibert SA, Steinchen W, Bange G</authors>
                <articleTitle>Structural basis for (p)ppGpp-mediated ribosomal large subunit arrest by RbgA</articleTitle>
                <journal>To Be Published</journal>
                <externalReference type="csd">0353</externalReference>
            </journalArticle>
        </primaryReference>
    </deposition>
    <map>
        <file type="map" format="CCP4" sizeKb="186625">emd_4359.map.gz</file>
        <dataType>Image stored as Reals</dataType>
        <dimensions>
            <numRows>360</numRows>
            <numColumns>360</numColumns>
            <numSections>360</numSections>
        </dimensions>
        <origin>
            <originRow>0.0</originRow>
            <originCol>0.0</originCol>
            <originSec>0.0</originSec>
        </origin>
        <limit>
            <limitRow>359.0</limitRow>
            <limitCol>359.0</limitCol>
            <limitSec>359.0</limitSec>
        </limit>
        <spacing>
            <spacingRow>360</spacingRow>
            <spacingCol>360</spacingCol>
            <spacingSec>360</spacingSec>
        </spacing>
        <cell>
            <cellA units="A">390.24</cellA>
            <cellB units="A">390.24</cellB>
            <cellC units="A">390.24</cellC>
            <cellAlpha units="degrees">90.0</cellAlpha>
            <cellBeta units="degrees">90.0</cellBeta>
            <cellGamma units="degrees">90.0</cellGamma>
        </cell>
        <axisOrder>
            <axisOrderFast>X</axisOrderFast>
            <axisOrderMedium>Y</axisOrderMedium>
            <axisOrderSlow>Z</axisOrderSlow>
        </axisOrder>
        <statistics>
            <minimum>-0.5041675</minimum>
            <maximum>1.0870134</maximum>
            <average>0.0018959928</average>
            <std>0.04716758</std>
        </statistics>
        <spaceGroupNumber>1</spaceGroupNumber>
        <details></details>
        <pixelSpacing>
            <pixelX units="A">1.084</pixelX>
            <pixelY units="A">1.084</pixelY>
            <pixelZ units="A">1.084</pixelZ>
        </pixelSpacing>
        <contourLevel source="author">0.114</contourLevel>
    </map>
    <sample>
        <numComponents>3</numComponents>
        <name>RbgA-GMPPNP-50S complex</name>
        <sampleComponentList>
            <sampleComponent componentID="1">
                <entry>protein</entry>
                <sciName>RbgA-GMPPNP-50S complex</sciName>
                <molWtTheo units="MDa">31.986</molWtTheo>
                <protein>
                    <sciSpeciesName ncbiTaxId="224308">Bacillus subtilis subsp. subtilis str. 168</sciSpeciesName>
                    <recombinantExpFlag>false</recombinantExpFlag>
                    <engSource>
                        <expSystem ncbiTaxId="469008">Escherichia coli BL21(DE3)</expSystem>
                    </engSource>
                </protein>
            </sampleComponent>
            <sampleComponent componentID="2">
                <entry>cellular-component</entry>
                <sciName>50S subunit</sciName>
                <cellular-component>
                    <sciSpeciesName ncbiTaxId="224308">Bacillus subtilis subsp. subtilis str. 168</sciSpeciesName>
                    <recombinantExpFlag>false</recombinantExpFlag>
                </cellular-component>
            </sampleComponent>
            <sampleComponent componentID="3">
                <entry>cellular-component</entry>
                <sciName>Ribosome Biogenesis GTPase A RbgA</sciName>
                <cellular-component>
                    <sciSpeciesName ncbiTaxId="224308">Bacillus subtilis subsp. subtilis str. 168</sciSpeciesName>
                    <recombinantExpFlag>false</recombinantExpFlag>
                    <engSource>
                        <expSystem ncbiTaxId="469008">Escherichia coli BL21(DE3)</expSystem>
                    </engSource>
                </cellular-component>
            </sampleComponent>
        </sampleComponentList>
    </sample>
    <experiment>
        <vitrification>
            <cryogenName>ETHANE</cryogenName>
            <details></details>
        </vitrification>
        <imaging>
            <electronSource>FIELD EMISSION GUN</electronSource>
            <electronDose units="e/A**2">2.5</electronDose>
            <imagingMode>BRIGHT FIELD</imagingMode>
            <illuminationMode>FLOOD BEAM</illuminationMode>
            <detector>FEI FALCON II (4k x 4k)</detector>
            <microscope>FEI TITAN KRIOS</microscope>
            <specimenHolderModel>OTHER</specimenHolderModel>
            <acceleratingVoltage units="kV">300</acceleratingVoltage>
        </imaging>
        <imageAcquisition/>
        <specimenPreparation>
            <specimenState>particle</specimenState>
            <buffer>
                <ph>7.5</ph>
            </buffer>
        </specimenPreparation>
    </experiment>
    <processing>
        <method>singleParticle</method>
        <reconstruction>
            <software>RELION</software>
            <resolutionByAuthor>3.65</resolutionByAuthor>
            <resolutionMethod>FSC 0.143 CUT-OFF</resolutionMethod>
        </reconstruction>
        <singleParticle>
            <numProjections>67875</numProjections>
        </singleParticle>
    </processing>
</emdEntry>
