2021-11-29 OBS PDBe PDBe PDBe 2018-02-27 2021-12-08 2021-12-08 2021-11-29 Suppressed Suppressed Suppressed Suppressed Suppressed X Suppressed X Suppressed X Suppressed X Suppressed X Suppressed X Suppressed X Suppressed X Suppressed X Suppressed X Suppressed X Suppressed X Suppressed Suppressed EMD-4320 associated EM volume
Suppressed
 6fus FULLOVERLAP Suppressed Suppressed 0 1 2 3
Suppressed
Suppressed Suppressed Suppressed 1 Suppressed Suppressed 1e-06
GPA in the sequence from -2 to 0 are encoded from the expression vector.
2 Suppressed LEVO X Suppressed Suppressed 1e-06 12 Suppressed LEVO X Suppressed Suppressed 1e-06 2 X singleParticle particle 0.000000059 7.9 10.0 KCl potasium chloride 40.0 NaCl sodium chloride 20.0 C8H18N2O4S HEPES 2.0 C9H15O6P TCEP 1.0 MgCl2 magnesium chloride
Solutions were made fresh and filtered through 0.2 um filter.
 Quantifoil, UltrAuFoil, R1.2/1.3 GOLD 300 CARBON HOLEY GLOW DISCHARGE AIR 0.02
grids were coated with graphene oxide prior to use
This sample was monodisperse from Sec-Mals column purification.
 FEI TITAN KRIOS OTHER BRIGHT FIELD FIELD EMISSION GUN 300 132.0 2.0 1.25 1.218 3.0 3.461 125000.0 130841.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN 70.0 100.0 Microscope was modified with a Cs corrector with two hexapole element FEI FALCON III (4k x 4k) COUNTING 4096 4096 5.0 1 917 0.8 27.925
Images were collected in movie-counting mode. 75 frames per movie stack, 60 second per movie stack
 1
The selected images were low-pass filtered at 20 Angstrom and normalized
482426
482426 were selected for initial 2D classification
 Gctf 9.03 gctf
Using SGD program inside Relion package to produce the initial model for 3D classification
 1 C2 FOURIER SPACE 3.18 FSC 0.143 CUT-OFF RELION V2 relion
Relion V2 was used for the reconstruction
58982 ANGULAR RECONSTITUTION RELION 2 relion ANGULAR RECONSTITUTION RELION 2 relion 200 2412.0 RELION 2 relion emd_4320.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 250 250 250 0 0 0 250 250 250 267.5 267.5 267.5 90.0 90.0 90.0 X Y Z -0.4335897 0.70426136 2.1938586e-05 0.020547332 1.07 1.07 1.07 0.108 AUTHOR Suppressed 1U1S A B 1-259 4JG3 C D E F G H I J K L M N FLEXIBLE FIT Cross-correlation coefficient REAL 90.0 emd_4320_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 250 250 250 0 0 0 250 250 250 267.5 267.5 267.5 90.0 90.0 90.0 X Y Z -0.048163787 0.08980633 1.0046427e-05 0.0033002219 1.07 1.07 1.07 AUTHOR Suppressed emd_4320_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 250 250 250 0 0 0 250 250 250 267.5 267.5 267.5 90.0 90.0 90.0 X Y Z -0.04460667 0.09723633 1.1892159e-05 0.003312789 1.07 1.07 1.07 AUTHOR Suppressed