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    <admin>
        <current_status>
            <date>2024-05-08</date>
            <code>OBS</code>
            <processing_site>RCSB</processing_site>
        </current_status>
        <sites>
            <deposition>RCSB</deposition>
            <last_processing>RCSB</last_processing>
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            <deposition>2023-10-18</deposition>
            <header_release>2024-04-17</header_release>
            <map_release>2024-04-17</map_release>
            <obsolete>2024-05-08</obsolete>
            <update>2024-05-08</update>
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            <entry>
                <date>2024-05-08</date>
                <entry>EMD-44587</entry>
            </entry>
        </superseded_by_list>
        <grant_support>
            <grant_reference>
                <funding_body>National Institutes of Health/National Cancer Institute (NIH/NCI)</funding_body>
                <code>CA116087</code>
                <country>United States</country>
            </grant_reference>
            <grant_reference>
                <funding_body>National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)</funding_body>
                <code>AI118932</code>
                <country>United States</country>
            </grant_reference>
            <grant_reference>
                <funding_body>National Institutes of Health/National Institute Of Allergy and Infectious Diseases (NIH/NIAID)</funding_body>
                <code>AI039657</code>
                <country>United States</country>
            </grant_reference>
        </grant_support>
        <title>Cryo-EM Structure of the Helicobacter pylori dcagT PR</title>
        <authors_list>
            <author>Roberts JR</author>
        </authors_list>
        <keywords>T4SS, protein translocation, MEMBRANE PROTEIN</keywords>
    </admin>
    <crossreferences>
        <citation_list>
            <primary_citation>
                <journal_citation published="true">
                    <author order="1">Roberts JR</author>
                    <author order="2">Tran SC</author>
                    <author order="3">Frick-Cheng AF</author>
                    <author order="4">Bryant KN</author>
                    <author order="5">Okoye CD</author>
                    <author order="6">Cover TL</author>
                    <author order="7">Ohi MD</author>
                    <title>Subdomains of the H. pylori Cag T4SS outer membrane core complex exhibit structural independence</title>
                    <journal_abbreviation>Life Sci Alliance</journal_abbreviation>
                    <country>US</country>
                    <year>2024</year>
                    <external_references type="DOI">doi:10.26508/lsa.202302560</external_references>
                    <external_references type="ISSN">2575-1077</external_references>
                </journal_citation>
            </primary_citation>
        </citation_list>
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                <emdb_id>EMD-42393</emdb_id>
                <relationship>
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            <emdb_reference>
                <emdb_id>EMD-42290</emdb_id>
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            <emdb_reference>
                <emdb_id>EMD-42392</emdb_id>
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    <sample>
        <name>Structure of the Helicobacter pylori dcagT PR</name>
        <supramolecule_list>
            <complex_supramolecule supramolecule_id="1">
                <name>Structure of the Helicobacter pylori dcagT PR</name>
                <parent>0</parent>
                <macromolecule_list>
                    <macromolecule>
                        <macromolecule_id>1</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>2</macromolecule_id>
                    </macromolecule>
                </macromolecule_list>
                <natural_source database="NCBI">
                    <organism ncbi="85962">Helicobacter pylori 26695</organism>
                </natural_source>
            </complex_supramolecule>
        </supramolecule_list>
        <macromolecule_list>
            <protein_or_peptide macromolecule_id="1">
                <name>Cag pathogenicity island protein (Cag8)</name>
                <natural_source database="NCBI">
                    <organism ncbi="85962">Helicobacter pylori 26695</organism>
                </natural_source>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>MGQAFFKKIV GCFCLGYLFL SSAIEAAALD IKNFNRGRVK VVNKKIAYLG DEKPITIWTS 
LDNVTVIQLE KDETISYITT GFNKGWSIVP NSNHIFIQPK SVKSNLMFEK EAVNFALMTR 
DYQEFLKTKK LIVDAPDPKE LEEQKKALEK EKEAKEQAQK AQKDKREKRK EERAKNRANL 
ENLTNAMSNP QNLSNNKNLS EFIKQQRENE LDQMERLEDM QEQAQANALK QIEELNKKQA 
EETIKQRAKD KINIKTDKPQ KSPEDNSIEL SPSDSAWRTN LVVRTNKALY QFILRIAQKD 
NFASAYLTVK LEYPQRHEVS SVIEELKKRE EAKRQKELIK QENLNTTAYI NRVMMASNEQ 
IINKEKIREE KQKIILDQAK ALETQYVHNA LKRNPVPRNY NYYQAPEKRS KHIMPSEIFD 
DGTFTYFGFK NITLQPAIFV VQPDGKLSMT DAAIDPNMTN SGLRWYRVNE IAEKFKLIKD 
KALVTVINKG YGKNPLTKNY NIKNYGELER VIKKLPEVRD K</string>
                </sequence>
            </protein_or_peptide>
            <protein_or_peptide macromolecule_id="2">
                <name>Cag pathogenicity island protein (Cag7)</name>
                <natural_source database="NCBI">
                    <organism ncbi="85962">Helicobacter pylori 26695</organism>
                </natural_source>
                <enantiomer>LEVO</enantiomer>
                <sequence>
                    <string>MNEENDKLET SKKAQQDSPQ DLSNEEATEA NHFENLLKES KESSDHHLDN PTETQTHFDG 
DKSEETQTQM DSEGNETSES SNGSLADKLF KKARKLVDNK KPFTQQKNLD EETQELNEED 
DQENNEYQEE TQTDLIDDET SKKTQQHSPQ DLSNEEATEA NHFENLLKES KESSDHHLDN 
PTETQTNFDG DKSEETQTQM DSEGNETSES SNGSLADKLF KKARKLVDNK KPFTQQKNLD 
EETQELNEED DQENNEYQEE TQTDLIDDET SKKTQQHSPQ DLSNEEATEA NHFENLLKES 
KESSDHHLDN PTETQTNFDG DKSEEITDDS NDQEIIKGSK KKYIIGGIVV AVLIVIILFS 
RSIFHYFMPL EDKSSRFSKD RNLYVNDEIQ IRQEYNRLLK ERNEKGNMID KNLFFNDDPN 
RTLYNYLNIA EIEDKNPLRA FYECISNGGN YEECLKLIKD KKLQDQMKKT LEAYNDCIKN 
AKTEEERIKC LDLIKDENLK KSLLNQQKVQ VALDCLKNAK TDEERNECLK LINDPEIREK 
FRKELELQKE LQEYKDCIKN AKTEAEKNKC LKGLSKEAIE RLKQQALDCL KNAKTDEERN 
ECLKNIPQDL QKELLADMSV KAYKDCVSKA RNEKEKQECE KLLTPEARKK LEQQVLDCLK 
NAKTDEERKK CLKDLPKDLQ SDILAKESLK AYKDCVSQAK TEAEKKECEK LLTPEAKKLL 
EEEAKESVKA YLDCVSQAKT EAEKKECEKL LTPEAKKKLE EAKKSVKAYL DCVSRARNEK 
EKKECEKLLT PEAKKLLEQQ ALDCLKNAKT DKERKKCLKD LPKDLQKKVL AKESVKAYLD 
CVSQAKTEAE KKECEKLLTP EARKLLEEAK KSVKAYLDCV SQAKTEAEKK ECEKLLTPEA 
RKLLEEXAKE SVKAYLDCVS QAKNEAEKKE CEKLLTLESK KKLEEAKKSV KAYLDCVSQA 
KTEAEKKECE KLLTPEAKKL LEQQALDCLK NAKTEADKKR CVKDLPKDLQ KKVLAKESLK 
AYKDCVSKAR NEKEKKECEK LLTPEAKKLL EEAKKSVKAY LDCVSQAKTE AEKKECEKLL 
TPEARKLLEE AKESVKAYKD CVSKARNEKE KKECEKLLTP EAKKLLEQQV LDCLKNAKTE 
ADKKRCVKDL PKDLQKKVLA KESVKAYLDC VSRARNEKEK KECEKLLTPE AKKLLEEAKE 
SLKAYKDCLS QARNEEERRA CEKLLTPEAR KLLEQEVKKS IKAYLDCVSR ARNEKEKKEC 
EKLLTPEARK FLAKQVLNCL EKAGNEEERK ACLKNLPKDL QENILAKESL KAYKDCLSQA 
RNEEERRACE KLLTPEARKL LEQEVKKSVK AYLDCVSRAR NEKEKKECEK LLTPEARKFL 
AKELQQKDKA IKDCLKNADP NDRAAIMKCL DGLSDEEKLK YLQEAREKAV ADCLAMAKTD 
EEKRKCQNLY SDLIQEIQNK RTQNKQNQLS KTERLHQASE CLDNLDDPTD QEAIEQCLEG 
LSDSERALIL GIKRQADEVD LIYSDLRNRK TFDNMAAKGY PLLPMDFKNG GDIATINATN 
VDADKIASDN PIYASIEPDI AKQYETEKTI KDKNLEAKLA KALGGNKKDD DKEKSKKSTA 
EAKAENNKID KDVAETAKNI SEIALKNKKE KSGEFVDENG NPIDDKKKAE KQDETSPVKQ 
AFIGKSDPTF VLAQYTPIEI TLTSKVDATL TGIVSGVVAK DVWNMNGTMI LLDKGTKVYG 
NYQSVKGGTP IMTRLMIVFT KAITPDGVII PLANAQAAGM LGEAGVDGYV NNHFMKRIGF 
AVIASVVNSF LQTAPIIALD KLIGLGKGRS ERTPEFNYAL GQAINGSMQS SAQMSNQILG 
QLMNIPPSFY KNEGDSIKIL TMDDIDFSGV YDVKITNKSV VDEIIKQSTK TLSREHEEIT 
TSPKGGN</string>
                </sequence>
            </protein_or_peptide>
        </macromolecule_list>
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            <method>singleParticle</method>
            <aggregation_state>particle</aggregation_state>
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                    <concentration units="mg/mL">.4</concentration>
                    <buffer>
                        <ph>8.0</ph>
                    </buffer>
                    <vitrification>
                        <cryogen_name>NITROGEN</cryogen_name>
                    </vitrification>
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                    <microscope>FEI TITAN KRIOS</microscope>
                    <illumination_mode>FLOOD BEAM</illumination_mode>
                    <imaging_mode>BRIGHT FIELD</imaging_mode>
                    <electron_source>FIELD EMISSION GUN</electron_source>
                    <acceleration_voltage units="kV">300</acceleration_voltage>
                    <nominal_defocus_min units="µm">1.0</nominal_defocus_min>
                    <nominal_defocus_max units="µm">2.5</nominal_defocus_max>
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                        <image_recording image_recording_id="1">
                            <film_or_detector_model>GATAN K3 (6k x 4k)</film_or_detector_model>
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                        <point_group>C17</point_group>
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                    <type>MAXIMUM LIKELIHOOD</type>
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