<?xml version="1.0" encoding="UTF-8"?>
<emd xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:noNamespaceSchemaLocation="https://ftp.ebi.ac.uk/pub/databases/em_ebi/emdb_related/emdb-schemas/emdb_schemas/v3/v3_0_9_0/emdb.xsd" version="3.0.9.0" emdb_id="EMD-41638">
    <admin>
        <current_status>
            <date>2023-12-13</date>
            <code>OBS</code>
            <processing_site>RCSB</processing_site>
        </current_status>
        <sites>
            <deposition>RCSB</deposition>
            <last_processing>RCSB</last_processing>
        </sites>
        <key_dates>
            <deposition>2023-08-18</deposition>
            <header_release>2023-10-11</header_release>
            <map_release>2023-10-11</map_release>
            <obsolete>2023-11-01</obsolete>
            <update>2023-12-13</update>
        </key_dates>
        <superseded_by_list>
            <entry>
                <date>2023-11-01</date>
                <entry>EMD-42429</entry>
            </entry>
        </superseded_by_list>
        <grant_support>
            <grant_reference>
                <funding_body>National Institutes of Health/National Heart, Lung, and Blood Institute (NIH/NHLBI)</funding_body>
                <code>HL013424</code>
                <country>United States</country>
            </grant_reference>
        </grant_support>
        <title>Plasminogen binding group A streptococcus M-like protein from AP53 bound to human plasminogen</title>
        <authors_list>
            <author ORCID="0000-0003-4674-1288">Readnour BM</author>
            <author ORCID="0000-0002-5453-6738">Tjia-Fleck SK</author>
            <author ORCID="0000-0003-0317-9539">Castellino FJ</author>
            <author ORCID="0000-0001-5730-2649">McCann NR</author>
        </authors_list>
        <keywords>PAM, human plasminogen, M-protein, Group A streptococcus, BLOOD CLOTTING</keywords>
    </admin>
    <crossreferences>
        <citation_list>
            <primary_citation>
                <journal_citation published="false">
                    <author ORCID="0000-0003-4674-1288" order="1">Readnour BM</author>
                    <author ORCID="0000-0003-4674-1288" order="2">Tjia-Fleck SK</author>
                    <author ORCID="0000-0001-5730-2649" order="3">McCann NR</author>
                    <author ORCID="0000-0001-7803-8058" order="4">Ayinuola YA</author>
                    <author ORCID="0000-0002-6506-764X" order="5">Ploplis VA</author>
                    <author ORCID="0000-0003-4674-1288" order="6">Castellino FJ</author>
                    <title>Plasminogen binding group A streptococcus M-like protein from AP53 bound to human plasminogen</title>
                    <journal_abbreviation>To Be Published</journal_abbreviation>
                    <external_references type="CSD">0353</external_references>
                </journal_citation>
            </primary_citation>
        </citation_list>
        <pdb_list>
            <pdb_reference>
                <pdb_id>8tvl</pdb_id>
                <relationship>
                    <in_frame>FULLOVERLAP</in_frame>
                </relationship>
            </pdb_reference>
        </pdb_list>
    </crossreferences>
    <sample>
        <name>Plasminogen Binding Group A Streptococcus M-Like Protein from AP53 bound to human plasminogen</name>
        <supramolecule_list>
            <complex_supramolecule supramolecule_id="1">
                <name>Plasminogen Binding Group A Streptococcus M-Like Protein from AP53 bound to human plasminogen</name>
                <parent>0</parent>
                <details>PAM was bound to the surface of a lentivirus</details>
                <natural_source database="NCBI">
                    <organism ncbi="1314">Streptococcus pyogenes</organism>
                    <strain>AP53</strain>
                    <cellular_location>Cell surface</cellular_location>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.041</theoretical>
                </molecular_weight>
            </complex_supramolecule>
        </supramolecule_list>
        <macromolecule_list>
            <protein_or_peptide macromolecule_id="1">
                <name>Plasminogen binding Group A streptococcus M-Like protein</name>
                <natural_source database="NCBI">
                    <organism ncbi="1314">Streptococcus pyogenes</organism>
                    <strain>AP53</strain>
                </natural_source>
                <recombinant_expression database="NCBI">
                    <recombinant_organism ncbi="9606">Homo sapiens</recombinant_organism>
                </recombinant_expression>
                <enantiomer>DEXTRO</enantiomer>
                <sequence>
                    <string>MNRADDARNEVLRGNLVRAELWYRQIQENDQL
KLENKGLKTDLREKEEELQGLKDDVEKLTADAELQRLKNERHEEAELERLKSERHDHDKK
EAERKALEDKLADKQEHLNGALRYINEKEAEAKEKEAEQKKLKEEKQISDASRQGLRRDL
DASREAKKQVEKDLANLTAELDKVKEEKQISDASRQGLRRDLDASREAKKQVEKGLANLT
AELDKVKEEKQISDASRQGLRRDLDASREAKKQVEKALEEANSKLAALEKLNKELEESKK
LTEKEKAELQAKLEAEAKALKEQLAKQAEELAKLRAEKASDSQTPDAKPGNKAVPGKGQA
PQAGTKPNQNKAPMKETKRQLPSTG</string>
                    <external_references type="UNIPROTKB">P49054</external_references>
                </sequence>
            </protein_or_peptide>
        </macromolecule_list>
    </sample>
    <structure_determination_list>
        <structure_determination structure_determination_id="1">
            <method>singleParticle</method>
            <aggregation_state>particle</aggregation_state>
            <specimen_preparation_list>
                <single_particle_preparation preparation_id="1">
                    <buffer>
                        <ph>7.4</ph>
                        <component>
                            <formula>PBS</formula>
                        </component>
                    </buffer>
                    <grid>
                        <model>EMS Lacey Carbon</model>
                        <material>GOLD</material>
                        <mesh>400</mesh>
                        <pretreatment>
                            <type>PLASMA CLEANING</type>
                            <time units="s">30</time>
                            <atmosphere>AIR</atmosphere>
                        </pretreatment>
                    </grid>
                    <vitrification>
                        <cryogen_name>ETHANE</cryogen_name>
                        <chamber_humidity units="percentage">100</chamber_humidity>
                        <chamber_temperature units="K">298</chamber_temperature>
                        <instrument>FEI VITROBOT MARK IV</instrument>
                    </vitrification>
                    <details>Plasminogen binding Group A streptococcus M like protein of AP53 bound to human plasminogen on the surface of a lentivirus</details>
                </single_particle_preparation>
            </specimen_preparation_list>
            <microscopy_list>
                <single_particle_microscopy microscopy_id="1">
                    <microscope>TFS KRIOS</microscope>
                    <illumination_mode>SPOT SCAN</illumination_mode>
                    <imaging_mode>BRIGHT FIELD</imaging_mode>
                    <electron_source>FIELD EMISSION GUN</electron_source>
                    <acceleration_voltage units="kV">300</acceleration_voltage>
                    <nominal_cs units="mm">2.7</nominal_cs>
                    <nominal_defocus_min units="µm">1.0</nominal_defocus_min>
                    <nominal_defocus_max units="µm">34.0</nominal_defocus_max>
                    <nominal_magnification>109000.0</nominal_magnification>
                    <specimen_holder_model>FEI TITAN KRIOS AUTOGRID HOLDER</specimen_holder_model>
                    <cooling_holder_cryogen>NITROGEN</cooling_holder_cryogen>
                    <alignment_procedure>
                        <basic/>
                    </alignment_procedure>
                    <image_recording_list>
                        <image_recording image_recording_id="1">
                            <film_or_detector_model>GATAN K3 (6k x 4k)</film_or_detector_model>
                            <number_grids_imaged>2</number_grids_imaged>
                            <number_real_images>4054</number_real_images>
                            <average_electron_dose_per_image units="e/Å^2">55.0</average_electron_dose_per_image>
                        </image_recording>
                    </image_recording_list>
                </single_particle_microscopy>
            </microscopy_list>
            <singleparticle_processing image_processing_id="1">
                <image_recording_id>1</image_recording_id>
                <startup_model type_of_model="NONE"/>
                <final_reconstruction>
                    <number_classes_used>14</number_classes_used>
                    <resolution units="Å" res_type="BY AUTHOR">3.8</resolution>
                    <resolution_method>FSC 0.143 CUT-OFF</resolution_method>
                    <number_images_used>212829</number_images_used>
                </final_reconstruction>
                <initial_angle_assignment>
                    <type>NOT APPLICABLE</type>
                </initial_angle_assignment>
                <final_angle_assignment>
                    <type>NOT APPLICABLE</type>
                </final_angle_assignment>
                <final_three_d_classification>
                    <number_classes>14</number_classes>
                </final_three_d_classification>
            </singleparticle_processing>
        </structure_determination>
    </structure_determination_list>
    <map format="CCP4" size_kbytes="186625">
        <file>emd_41638.map.gz</file>
        <symmetry>
            <space_group>1</space_group>
        </symmetry>
        <data_type>IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)</data_type>
        <dimensions>
            <col>360</col>
            <row>360</row>
            <sec>360</sec>
        </dimensions>
        <origin>
            <col>0</col>
            <row>0</row>
            <sec>0</sec>
        </origin>
        <spacing>
            <x>360</x>
            <y>360</y>
            <z>360</z>
        </spacing>
        <cell>
            <a units="Å">157.32</a>
            <b units="Å">157.32</b>
            <c units="Å">157.32</c>
            <alpha units="deg">90.0</alpha>
            <beta units="deg">90.0</beta>
            <gamma units="deg">90.0</gamma>
        </cell>
        <axis_order>
            <fast>X</fast>
            <medium>Y</medium>
            <slow>Z</slow>
        </axis_order>
        <statistics>
            <minimum>-14.081867000000001</minimum>
            <maximum>15.476846</maximum>
            <average>-0.012572726</average>
            <std>0.73909646</std>
        </statistics>
        <pixel_spacing>
            <x units="Å">0.437</x>
            <y units="Å">0.437</y>
            <z units="Å">0.437</z>
        </pixel_spacing>
        <contour_list>
            <contour primary="true">
                <level>2.3</level>
                <source>AUTHOR</source>
            </contour>
        </contour_list>
        <label>::::EMDATABANK.org::::EMD-41638::::</label>
        <annotation_details>Plasminogen binding Group A streptococcus M like protein from AP53 bound to plasminogen full map.</annotation_details>
    </map>
    <interpretation>
        <modelling_list>
            <modelling>
                <refinement_protocol>RIGID BODY FIT</refinement_protocol>
                <refinement_space>REAL</refinement_space>
            </modelling>
        </modelling_list>
        <half_map_list>
            <half_map format="CCP4" size_kbytes="186625">
                <file>emd_41638_half_map_1.map.gz</file>
                <symmetry>
                    <space_group>1</space_group>
                </symmetry>
                <data_type>IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)</data_type>
                <dimensions>
                    <col>360</col>
                    <row>360</row>
                    <sec>360</sec>
                </dimensions>
                <origin>
                    <col>0</col>
                    <row>0</row>
                    <sec>0</sec>
                </origin>
                <spacing>
                    <x>360</x>
                    <y>360</y>
                    <z>360</z>
                </spacing>
                <cell>
                    <a units="Å">157.32</a>
                    <b units="Å">157.32</b>
                    <c units="Å">157.32</c>
                    <alpha units="deg">90.0</alpha>
                    <beta units="deg">90.0</beta>
                    <gamma units="deg">90.0</gamma>
                </cell>
                <axis_order>
                    <fast>X</fast>
                    <medium>Y</medium>
                    <slow>Z</slow>
                </axis_order>
                <statistics>
                    <minimum>-0.09546966</minimum>
                    <maximum>0.111528724</maximum>
                    <average>0.00040332918</average>
                    <std>0.0115785925</std>
                </statistics>
                <pixel_spacing>
                    <x units="Å">0.437</x>
                    <y units="Å">0.437</y>
                    <z units="Å">0.437</z>
                </pixel_spacing>
                <contour_list>
                    <contour primary="true">
                        <source>AUTHOR</source>
                    </contour>
                </contour_list>
                <label>::::EMDATABANK.org::::EMD-41638::::</label>
                <annotation_details>Plasminogen binding Group A streptococcus M like protein from AP53 bound to plasminogen half map.</annotation_details>
            </half_map>
            <half_map format="CCP4" size_kbytes="186625">
                <file>emd_41638_half_map_2.map.gz</file>
                <symmetry>
                    <space_group>1</space_group>
                </symmetry>
                <data_type>IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)</data_type>
                <dimensions>
                    <col>360</col>
                    <row>360</row>
                    <sec>360</sec>
                </dimensions>
                <origin>
                    <col>0</col>
                    <row>0</row>
                    <sec>0</sec>
                </origin>
                <spacing>
                    <x>360</x>
                    <y>360</y>
                    <z>360</z>
                </spacing>
                <cell>
                    <a units="Å">157.32</a>
                    <b units="Å">157.32</b>
                    <c units="Å">157.32</c>
                    <alpha units="deg">90.0</alpha>
                    <beta units="deg">90.0</beta>
                    <gamma units="deg">90.0</gamma>
                </cell>
                <axis_order>
                    <fast>X</fast>
                    <medium>Y</medium>
                    <slow>Z</slow>
                </axis_order>
                <statistics>
                    <minimum>-0.092283934</minimum>
                    <maximum>0.1116757</maximum>
                    <average>0.00038104507</average>
                    <std>0.011600675</std>
                </statistics>
                <pixel_spacing>
                    <x units="Å">0.437</x>
                    <y units="Å">0.437</y>
                    <z units="Å">0.437</z>
                </pixel_spacing>
                <contour_list>
                    <contour primary="true">
                        <source>AUTHOR</source>
                    </contour>
                </contour_list>
                <label>::::EMDATABANK.org::::EMD-41638::::</label>
                <annotation_details>Plasminogen binding Group A streptococcus M like protein from AP53 bound to plasminogen half map.</annotation_details>
            </half_map>
        </half_map_list>
    </interpretation>
</emd>
