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<emdEntry accessCode="4004" version="1.9.6">
    <admin>
        <lastUpdate>2017-07-26</lastUpdate>
    </admin>
    <deposition>
        <status>OBS</status>
        <depositionDate>2016-05-30</depositionDate>
        <depositionSite>PDBe</depositionSite>
        <processingSite>PDBe</processingSite>
        <headerReleaseDate>2016-10-12</headerReleaseDate>
        <mapReleaseDate>2017-06-28</mapReleaseDate>
        <obsoletedDate>2017-07-26</obsoletedDate>
        <title>Tail sheath and tail tube of native bacteriophage phi812K1-420</title>
        <authors>Novacek J, Siborova M, Benesik M, Pantucek R, Doskar J, Plevka P</authors>
        <primaryReference published="false">
            <journalArticle>
                <authors>Novacek J, Siborova M, Benesik M, Pantucek R, Doskar J, Plevka P</authors>
                <articleTitle>Structure and genome release of Myoviridae phage with double layered baseplate</articleTitle>
                <journal>to be published</journal>
                <externalReference type="csd">0353</externalReference>
            </journalArticle>
        </primaryReference>
    </deposition>
    <map>
        <file type="map" format="CCP4" sizeKb="34461">emd_4004.map.gz</file>
        <dataType>Image stored as Reals</dataType>
        <dimensions>
            <numRows>205</numRows>
            <numColumns>205</numColumns>
            <numSections>205</numSections>
        </dimensions>
        <origin>
            <originRow>-101.0</originRow>
            <originCol>-101.0</originCol>
            <originSec>-101.0</originSec>
        </origin>
        <limit>
            <limitRow>103.0</limitRow>
            <limitCol>103.0</limitCol>
            <limitSec>103.0</limitSec>
        </limit>
        <spacing>
            <spacingRow>205</spacingRow>
            <spacingCol>205</spacingCol>
            <spacingSec>205</spacingSec>
        </spacing>
        <cell>
            <cellA units="A">282.9</cellA>
            <cellB units="A">282.9</cellB>
            <cellC units="A">282.9</cellC>
            <cellAlpha units="degrees">90.0</cellAlpha>
            <cellBeta units="degrees">90.0</cellBeta>
            <cellGamma units="degrees">90.0</cellGamma>
        </cell>
        <axisOrder>
            <axisOrderFast>X</axisOrderFast>
            <axisOrderMedium>Y</axisOrderMedium>
            <axisOrderSlow>Z</axisOrderSlow>
        </axisOrder>
        <statistics>
            <minimum>-0.1775362</minimum>
            <maximum>4.589265</maximum>
            <average>0.1678828</average>
            <std>0.51724523</std>
        </statistics>
        <spaceGroupNumber>1</spaceGroupNumber>
        <details></details>
        <pixelSpacing>
            <pixelX units="A">1.38</pixelX>
            <pixelY units="A">1.38</pixelY>
            <pixelZ units="A">1.38</pixelZ>
        </pixelSpacing>
        <contourLevel source="author">1.7</contourLevel>
        <annotationDetails>tail sheath and tail tube of native bacteriophage phi812K1-420</annotationDetails>
    </map>
    <sample>
        <numComponents>1</numComponents>
        <name>Staphylococcus phage 812</name>
        <sampleComponentList>
            <sampleComponent componentID="1">
                <entry>virus</entry>
                <sciName>Staphylococcus phage 812</sciName>
                <virus>
                    <sciSpeciesName ncbiTaxId="307898">Staphylococcus phage 812</sciSpeciesName>
                    <sciSpeciesStrain>K420</sciSpeciesStrain>
                    <empty>false</empty>
                    <enveloped>false</enveloped>
                    <isolate>SPECIES</isolate>
                    <class>VIRION</class>
                    <natSource>
                        <hostSpecies ncbiTaxId="1280">Staphylococcus aureus</hostSpecies>
                    </natSource>
                    <shell id="1">
                        <diameter units="A">1100.0</diameter>
                        <tNumber>16</tNumber>
                    </shell>
                </virus>
            </sampleComponent>
        </sampleComponentList>
    </sample>
    <experiment>
        <vitrification>
            <cryogenName>ETHANE</cryogenName>
            <humidity>100</humidity>
            <temperature units="Kelvin">295</temperature>
            <instrument>FEI VITROBOT MARK IV</instrument>
            <details></details>
        </vitrification>
        <imaging>
            <electronSource>FIELD EMISSION GUN</electronSource>
            <electronDose units="e/A**2">20.0</electronDose>
            <imagingMode>BRIGHT FIELD</imagingMode>
            <illuminationMode>FLOOD BEAM</illuminationMode>
            <detector>FEI FALCON II (4k x 4k)</detector>
            <nominalCs units="mm">2.7</nominalCs>
            <microscope>FEI TITAN KRIOS</microscope>
            <specimenHolderModel>FEI TITAN KRIOS AUTOGRID HOLDER</specimenHolderModel>
            <acceleratingVoltage units="kV">300</acceleratingVoltage>
        </imaging>
        <imageAcquisition/>
        <specimenPreparation>
            <specimenState>particle</specimenState>
            <specimenConc units="mg/ml">1</specimenConc>
            <buffer>
                <ph>8.0</ph>
            </buffer>
            <helicalParameters>
                <deltaPhi units="degrees">21.4</deltaPhi>
                <deltaZ units="A">38.9</deltaZ>
                <axialSymmetry>C6</axialSymmetry>
            </helicalParameters>
        </specimenPreparation>
    </experiment>
    <processing>
        <method>helical</method>
        <reconstruction>
            <algorithm>BACK PROJECTION</algorithm>
            <software>SPIDER</software>
            <resolutionByAuthor>6.2</resolutionByAuthor>
            <resolutionMethod>FSC 0.143 CUT-OFF</resolutionMethod>
        </reconstruction>
        <helical/>
    </processing>
</emdEntry>
