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        <current_status>
            <date>2017-07-26</date>
            <code>OBS</code>
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        <sites>
            <deposition>PDBe</deposition>
            <last_processing>PDBe</last_processing>
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        <key_dates>
            <deposition>2016-05-30</deposition>
            <header_release>2016-10-12</header_release>
            <map_release>2017-06-28</map_release>
            <obsolete>2017-07-26</obsolete>
            <update>2017-07-26</update>
        </key_dates>
        <title>Tail sheath and tail tube of native bacteriophage phi812K1-420</title>
        <authors_list>
            <author>Novacek J</author>
            <author>Siborova M</author>
            <author>Benesik M</author>
            <author>Pantucek R</author>
            <author>Doskar J</author>
            <author>Plevka P</author>
        </authors_list>
    </admin>
    <crossreferences>
        <citation_list>
            <primary_citation>
                <journal_citation published="false">
                    <author order="1">Novacek J</author>
                    <author order="2">Siborova M</author>
                    <author order="3">Benesik M</author>
                    <author order="4">Pantucek R</author>
                    <author order="5">Doskar J</author>
                    <author order="6">Plevka P</author>
                    <title>Structure and genome release of Myoviridae phage with double layered baseplate</title>
                    <journal_abbreviation>to be published</journal_abbreviation>
                    <external_references type="CSD">0353</external_references>
                </journal_citation>
            </primary_citation>
        </citation_list>
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                <emdb_id>EMD-4004</emdb_id>
                <relationship>
                    <other>associated EM volume</other>
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        <name>Staphylococcus phage 812</name>
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                <name>Staphylococcus phage 812</name>
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                <sci_species_name ncbi="307898">Staphylococcus phage 812</sci_species_name>
                <sci_species_strain>K420</sci_species_strain>
                <natural_host database="NCBI">
                    <organism ncbi="1280">Staphylococcus aureus</organism>
                </natural_host>
                <virus_shell shell_id="1">
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                    <triangulation>16</triangulation>
                </virus_shell>
                <virus_type>VIRION</virus_type>
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                <virus_enveloped>false</virus_enveloped>
                <virus_empty>false</virus_empty>
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                        <ph>8.</ph>
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                            <formula>TrisHCl</formula>
                            <name>tris</name>
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                            <concentration units="mM">10.0</concentration>
                            <formula>NaCl</formula>
                            <name>sodium chloride</name>
                        </component>
                        <component>
                            <concentration units="mM">10.0</concentration>
                            <formula>CaCl2</formula>
                            <name>calcium chloride</name>
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                        <material>COPPER</material>
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                            <type>GLOW DISCHARGE</type>
                            <atmosphere>AIR</atmosphere>
                        </pretreatment>
                    </grid>
                    <vitrification>
                        <cryogen_name>ETHANE</cryogen_name>
                        <chamber_humidity units="percentage">100</chamber_humidity>
                        <chamber_temperature units="K">295</chamber_temperature>
                        <instrument>FEI VITROBOT MARK IV</instrument>
                        <details></details>
                    </vitrification>
                </helical_preparation>
            </specimen_preparation_list>
            <microscopy_list>
                <helical_microscopy microscopy_id="1">
                    <microscope>FEI TITAN KRIOS</microscope>
                    <illumination_mode>FLOOD BEAM</illumination_mode>
                    <imaging_mode>BRIGHT FIELD</imaging_mode>
                    <electron_source>FIELD EMISSION GUN</electron_source>
                    <acceleration_voltage units="kV">300</acceleration_voltage>
                    <c2_aperture_diameter units="µm">100.0</c2_aperture_diameter>
                    <nominal_cs units="mm">2.7</nominal_cs>
                    <specimen_holder_model>FEI TITAN KRIOS AUTOGRID HOLDER</specimen_holder_model>
                    <cooling_holder_cryogen>NITROGEN</cooling_holder_cryogen>
                    <alignment_procedure>
                        <coma_free/>
                    </alignment_procedure>
                    <image_recording_list>
                        <image_recording image_recording_id="1">
                            <film_or_detector_model>FEI FALCON II (4k x 4k)</film_or_detector_model>
                            <detector_mode>INTEGRATING</detector_mode>
                            <digitization_details/>
                            <average_exposure_time units="s">0.86</average_exposure_time>
                            <average_electron_dose_per_image units="e/Å^2">20.0</average_electron_dose_per_image>
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                            <delta_z units="Å">38.9</delta_z>
                            <delta_phi units="deg">21.4</delta_phi>
                            <axial_symmetry>C6</axial_symmetry>
                        </helical_parameters>
                    </applied_symmetry>
                    <algorithm>BACK PROJECTION</algorithm>
                    <resolution units="Å" res_type="BY AUTHOR">6.2</resolution>
                    <resolution_method>FSC 0.143 CUT-OFF</resolution_method>
                    <software_list>
                        <software>
                            <name>SPIDER</name>
                        </software>
                    </software_list>
                    <number_images_used>2210</number_images_used>
                </final_reconstruction>
                <ctf_correction>
                    <software_list>
                        <software>
                            <name>CTFFIND</name>
                            <version>4</version>
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                        <software>
                            <name>SPIDER</name>
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                    <type>NOT APPLICABLE</type>
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                            <name>SPIDER</name>
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                        <software>
                            <name>IHRSR++</name>
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        <annotation_details>tail sheath and tail tube of native bacteriophage phi812K1-420</annotation_details>
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