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<emdEntry accessCode="3676" version="1.9.6" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xsi:schemaLocation="https://github.com/emdb-empiar/emdb-schemas/blob/master/v1/v1_9/emdb_v1_9_6.xsd">
    <admin>
        <lastUpdate>2019-05-01</lastUpdate>
    </admin>
    <deposition>
        <status>OBS</status>
        <depositionDate>2017-04-19</depositionDate>
        <depositionSite>PDBe</depositionSite>
        <processingSite>PDBe</processingSite>
        <headerReleaseDate>2018-01-24</headerReleaseDate>
        <mapReleaseDate>2018-05-23</mapReleaseDate>
        <obsoletedDate>2019-05-01</obsoletedDate>
        <title>Atomic Structure of the Broad Bean Stain Virus (BBSV) by cryo-EM</title>
        <authors>Lecorre F, Bron P, Lai Kee Him J</authors>
        <fittedPDBEntryIdList>
            <fittedPDBEntryId>5npx</fittedPDBEntryId>
        </fittedPDBEntryIdList>
        <primaryReference published="false">
            <journalArticle>
                <authors>Lecorre F, Lai-Kee-Him J, Blanc S, Zeddam JL, Trapani S, Bron P</authors>
                <articleTitle>The atomic structure of Broad Bean Stain Virus revealed by cryo-electron microscopy</articleTitle>
                <journal>To Be Published</journal>
                <externalReference type="csd">0353</externalReference>
            </journalArticle>
        </primaryReference>
    </deposition>
    <map>
        <file format="CCP4" sizeKb="237277" type="map">emd_3676.map.gz</file>
        <dataType>Image stored as Reals</dataType>
        <dimensions>
            <numRows>390</numRows>
            <numColumns>390</numColumns>
            <numSections>390</numSections>
        </dimensions>
        <origin>
            <originRow>-195.0</originRow>
            <originCol>-195.0</originCol>
            <originSec>-195.0</originSec>
        </origin>
        <limit>
            <limitRow>194.0</limitRow>
            <limitCol>194.0</limitCol>
            <limitSec>194.0</limitSec>
        </limit>
        <spacing>
            <spacingRow>390</spacingRow>
            <spacingCol>390</spacingCol>
            <spacingSec>390</spacingSec>
        </spacing>
        <cell>
            <cellA units="A">432.9</cellA>
            <cellB units="A">432.9</cellB>
            <cellC units="A">432.9</cellC>
            <cellAlpha units="degrees">90.0</cellAlpha>
            <cellBeta units="degrees">90.0</cellBeta>
            <cellGamma units="degrees">90.0</cellGamma>
        </cell>
        <axisOrder>
            <axisOrderFast>X</axisOrderFast>
            <axisOrderMedium>Y</axisOrderMedium>
            <axisOrderSlow>Z</axisOrderSlow>
        </axisOrder>
        <statistics>
            <minimum>-0.26571634</minimum>
            <maximum>0.40366957</maximum>
            <average>0.0014288375</average>
            <std>0.022530619</std>
        </statistics>
        <spaceGroupNumber>1</spaceGroupNumber>
        <details />
        <pixelSpacing>
            <pixelX units="A">1.11</pixelX>
            <pixelY units="A">1.11</pixelY>
            <pixelZ units="A">1.11</pixelZ>
        </pixelSpacing>
        <contourLevel source="author">0.0625</contourLevel>
    </map>
    <sample>
        <numComponents>3</numComponents>
        <name>Broad bean stain virus</name>
        <sampleComponentList>
            <sampleComponent componentID="1">
                <entry>virus</entry>
                <sciName>Broad bean stain virus</sciName>
                <virus>
                    <sciSpeciesName ncbiTaxId="593572">Broad bean stain virus</sciSpeciesName>
                    <empty>false</empty>
                    <enveloped>false</enveloped>
                    <isolate>STRAIN</isolate>
                    <class>VIRION</class>
                    <natSource>
                        <hostSpecies ncbiTaxId="3906">Vicia faba</hostSpecies>
                    </natSource>
                    <shell id="1">
                        <nameElement>capsid</nameElement>
                        <diameter units="A">300.0</diameter>
                        <tNumber>1</tNumber>
                    </shell>
                </virus>
            </sampleComponent>
            <sampleComponent componentID="2">
                <entry>protein</entry>
                <sciName>Polyprotein</sciName>
                <molWtTheo units="MDa">0.041247977</molWtTheo>
                <protein>
                    <sciSpeciesName ncbiTaxId="593572">Broad bean stain virus</sciSpeciesName>
                    <numCopies>1</numCopies>
                    <recombinantExpFlag>false</recombinantExpFlag>
                </protein>
            </sampleComponent>
            <sampleComponent componentID="3">
                <entry>protein</entry>
                <sciName>Polyprotein</sciName>
                <molWtTheo units="MDa">0.023589662</molWtTheo>
                <protein>
                    <sciSpeciesName ncbiTaxId="593572">Broad bean stain virus</sciSpeciesName>
                    <numCopies>1</numCopies>
                    <recombinantExpFlag>false</recombinantExpFlag>
                </protein>
            </sampleComponent>
        </sampleComponentList>
    </sample>
    <experiment>
        <vitrification>
            <cryogenName>ETHANE</cryogenName>
            <humidity>99</humidity>
            <temperature units="Kelvin">298.15</temperature>
            <instrument>GATAN CRYOPLUNGE 3</instrument>
            <details>blot for 1 second before plunging. </details>
        </vitrification>
        <imaging>
            <electronSource>FIELD EMISSION GUN</electronSource>
            <electronDose units="e/A**2">45.0</electronDose>
            <imagingMode>BRIGHT FIELD</imagingMode>
            <illuminationMode>FLOOD BEAM</illuminationMode>
            <detector>FEI FALCON II (4k x 4k)</detector>
            <microscope>FEI TITAN KRIOS</microscope>
            <specimenHolderModel>FEI TITAN KRIOS AUTOGRID HOLDER</specimenHolderModel>
            <acceleratingVoltage units="kV">300</acceleratingVoltage>
            <nominalMagnification>59000.0</nominalMagnification>
        </imaging>
        <imageAcquisition>
            <numDigitalImages>2899</numDigitalImages>
            <samplingSize units="microns">14.0</samplingSize>
            <details>1779 images were retained for 3D reconstruction</details>
        </imageAcquisition>
        <fitting>
            <pdbEntryIdList />
            <refProtocol>flexible</refProtocol>
            <refSpace>RECIPROCAL</refSpace>
        </fitting>
        <specimenPreparation>
            <specimenState>particle</specimenState>
            <specimenConc units="mg/ml">2</specimenConc>
            <buffer>
                <ph>7.4</ph>
            </buffer>
        </specimenPreparation>
    </experiment>
    <processing>
        <method>singleParticle</method>
        <reconstruction>
            <algorithm>BACK PROJECTION</algorithm>
            <software>RELION</software>
            <resolutionByAuthor>3.8</resolutionByAuthor>
            <resolutionMethod>FSC 0.143 CUT-OFF</resolutionMethod>
        </reconstruction>
        <singleParticle>
            <appliedSymmetry>I</appliedSymmetry>
            <numProjections>36261</numProjections>
        </singleParticle>
    </processing>
</emdEntry>