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    <admin>
        <lastUpdate>2019-03-27</lastUpdate>
    </admin>
    <deposition>
        <status>OBS</status>
        <depositionDate>2017-03-21</depositionDate>
        <depositionSite>PDBe</depositionSite>
        <processingSite>PDBe</processingSite>
        <headerReleaseDate>2017-04-19</headerReleaseDate>
        <obsoletedDate>2019-03-27</obsoletedDate>
        <title>Negative staining of antibodies cooperative complex formed by human IgG1 mAb7B10 and mAb2C1 bound to the N. meningitidis antigen factor H binding protein (fHbp)</title>
        <authors>Peschiera I, Ferlenghi I, Melero R, Liljeroos LJ, Paccagnini E, Giusti F, Carazo JM, Sorzano COS, Scarselli M</authors>
        <primaryReference published="false">
            <journalArticle>
                <authors>Peschiera I, Giuliani M, Giusti F, Melero R, Paccagnini E, Donnarumma D, Pansegrau W, Carazo JM, Sorzano COS, Scarselli M, Masignani V, Liljeroos LJ, Ferlenghi I</authors>
                <articleTitle>Structural basis for cooperativity of human monoclonal antibodies to meningococcal factor H binding protein</articleTitle>
                <journal>To Be Published</journal>
                <externalReference type="csd">0353</externalReference>
            </journalArticle>
        </primaryReference>
    </deposition>
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        <dataType>Image stored as Reals</dataType>
        <dimensions>
            <numRows>60</numRows>
            <numColumns>60</numColumns>
            <numSections>60</numSections>
        </dimensions>
        <origin>
            <originRow>-27.0</originRow>
            <originCol>-27.0</originCol>
            <originSec>-27.0</originSec>
        </origin>
        <limit>
            <limitRow>32.0</limitRow>
            <limitCol>32.0</limitCol>
            <limitSec>32.0</limitSec>
        </limit>
        <spacing>
            <spacingRow>60</spacingRow>
            <spacingCol>60</spacingCol>
            <spacingSec>60</spacingSec>
        </spacing>
        <cell>
            <cellA units="A">396.0</cellA>
            <cellB units="A">396.0</cellB>
            <cellC units="A">396.0</cellC>
            <cellAlpha units="degrees">90.0</cellAlpha>
            <cellBeta units="degrees">90.0</cellBeta>
            <cellGamma units="degrees">90.0</cellGamma>
        </cell>
        <axisOrder>
            <axisOrderFast>X</axisOrderFast>
            <axisOrderMedium>Y</axisOrderMedium>
            <axisOrderSlow>Z</axisOrderSlow>
        </axisOrder>
        <statistics>
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            <maximum>0.24199128</maximum>
            <average>0.00020917485</average>
            <std>0.013542563</std>
        </statistics>
        <spaceGroupNumber>1</spaceGroupNumber>
        <details></details>
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        </pixelSpacing>
        <contourLevel source="author">0.0431</contourLevel>
        <annotationDetails>Negative staining of cooperative couple of human IgG1 mAb7B10 and mAb2C1 bound to N.meningitidis antigen fHbp</annotationDetails>
    </map>
    <sample>
        <numComponents>2</numComponents>
        <name>Quaternary complex formed by mAb7B10, mAb2C1 and two molecules of fHbp</name>
        <details>Cooperative couple of human IgG1 mAbs bound to an antigen</details>
        <sampleComponentList>
            <sampleComponent componentID="1">
                <entry>protein</entry>
                <sciName>Quaternary complex formed by mAb7B10, mAb2C1 and two molecules of fHbp</sciName>
                <details>Cooperative couple of human IgG1 mAbs bound to an antigen</details>
                <protein>
                    <sciSpeciesName ncbiTaxId="9606">Homo sapiens</sciSpeciesName>
                    <recombinantExpFlag>false</recombinantExpFlag>
                    <engSource>
                        <expSystem ncbiTaxId="40674">Mammalia</expSystem>
                    </engSource>
                </protein>
            </sampleComponent>
            <sampleComponent componentID="2">
                <entry>cellular-component</entry>
                <sciName>N.meningitidis antigen factor H binding protein (fHbp)</sciName>
                <cellular-component>
                    <sciSpeciesName ncbiTaxId="491">Neisseria meningitidis serogroup B</sciSpeciesName>
                    <recombinantExpFlag>false</recombinantExpFlag>
                    <engSource>
                        <expSystem ncbiTaxId="469008">Escherichia coli BL21(DE3)</expSystem>
                    </engSource>
                </cellular-component>
            </sampleComponent>
        </sampleComponentList>
    </sample>
    <experiment>
        <vitrification>
            <cryogenName>NONE</cryogenName>
        </vitrification>
        <imaging>
            <electronSource>FIELD EMISSION GUN</electronSource>
            <electronDose units="e/A**2">30.0</electronDose>
            <imagingMode>BRIGHT FIELD</imagingMode>
            <illuminationMode>FLOOD BEAM</illuminationMode>
            <detector>TVIPS TEMCAM-F224 (2k x 2k)</detector>
            <microscope>FEI/PHILIPS CM200FEG</microscope>
            <specimenHolderModel>OTHER</specimenHolderModel>
            <acceleratingVoltage units="kV">200</acceleratingVoltage>
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        <imageAcquisition/>
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            <specimenState>particle</specimenState>
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                <ph>8.0</ph>
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        <method>singleParticle</method>
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            <resolutionByAuthor>28.0</resolutionByAuthor>
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            <numProjections>10000</numProjections>
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