2016-10-05 OBS 2015-12-08 PDBe PDBe 2015-12-16 2016-10-05
At authors request.
Human mithocondrial Lon protease lacking the first 271 amino acids. Kereiche S, Kovacik L, Kutejova E, Bednar J, Pevala V, Ambro L, Bellova J, Raska I AAA, Lon protease, Human Kereiche S, Kovacik L, Kutejova E, Bednar J, Pevala V, Ambro L, Bellova J, Raska I Conformational change of Human mitochondrial Lon protease NAT.STRUCT.BIOL. emd_3273.map.gz Image stored as Reals 192 192 192 0 0 0 191 191 191 192 192 192 272.63998 272.63998 272.63998 90.0 90.0 90.0 X Y Z -0.02581789 0.05747430 0.00040323 0.00578300 1
::::EMDATABANK.org::::EMD-3273::::
1.42 1.42 1.42 0.0205 Reconstruction of the Human Lon protease lacking the first 271 amino acids.
emd_3273.png
1 Human mitochondrial Lon protease D271 0.43 0.43 protein human Lon protease LonP1 0.43 0.43 Homo sapiens true human P36776 mitochondria mitochondria Escherichia coli DE3 blot 2 seconds before plunging ETHANE 100 FEI VITROBOT MARK IV 90 FIELD EMISSION GUN 10 BRIGHT FIELD 1300 2400 FLOOD BEAM FEI FALCON II (4k x 4k) 0 98682 FEI TITAN KRIOS 04-APR-2014 FEI TITAN KRIOS AUTOGRID HOLDER 300 47000 2980 14 16
The domains were separately fitted by interactive docking in the situs programme. Only five copies could be fitted.
sculptor, situs, Chimera rigid body REAL 3M6A A particle 1 200 mesh quanti-foil R2/2 with thin carbon support, glow discharged in standard vaccum atmosphere
20 mM HEPES, 150 mM NaCl, 5 mM MgCl2
8 singleParticle RELION RELION ctffind3 20.9 FSC 0.143, gold-standard 20 C1 39800