HPUB OBS PDBe PDBe PDBe 2015-12-08 2015-12-16 2016-10-05 2016-10-05 Human mithocondrial Lon protease lacking the first 271 amino acids. Kereiche S Kovacik L Kutejova E Bednar J Pevala V Ambro L Bellova J Raska I AAA, Lon protease, Human Kereiche S Kovacik L Kutejova E Bednar J Pevala V Ambro L Bellova J Raska I Conformational change of Human mitochondrial Lon protease NAT.STRUCT.BIOL. Human mitochondrial Lon protease D271 Human mitochondrial Lon protease D271 1 0.43 0.43 human Lon protease Homo sapiens human mitochondria mitochondria 0.43 0.43 true Escherichia coli DE3 P36776 singleParticle particle 1 8.0
20 mM HEPES, 150 mM NaCl, 5 mM MgCl2
200 mesh quanti-foil R2/2 with thin carbon support, glow discharged in standard vaccum atmosphere
 ETHANE 100 90 FEI VITROBOT MARK IV blot 2 seconds before plunging FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 0 1.3 2.4 47000.0 98682.0 FEI TITAN KRIOS AUTOGRID HOLDER 2014-04-04 FEI FALCON II (4k x 4k) 14 2980 10 16.0
ctffind3
 OTHER 20.9 OTHER RELION 39800 20 emd_3273.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 192 192 192 0 0 0 192 192 192 272.63998 272.63998 272.63998 90.0 90.0 90.0 X Y Z -0.02581789 0.0574743 0.00040323 0.005783 1.42 1.42 1.42 0.0205 AUTHOR Reconstruction of the Human Lon protease lacking the first 271 amino acids.
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 3M6A A RIGID BODY FIT sculptor, situs, Chimera
The domains were separately fitted by interactive docking in the situs programme. Only five copies could be fitted.
REAL
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