2017-05-03 OBS 2015-11-04 PDBe PDBe 2015-11-18 2017-05-03 2017-05-03 EMD-4060
map is obsoleate
Negative Stain electron microscopy of GIF:IL2 complex Felix J, Kandiah E, van Zundert GCP, Pauwels K, Novanska K, Verstraete K, Bloch Y, Read RJ, Vergauwen B, Gutsche I, Savvides SN immune system, ORF virus, cytokines, cytokine receptors Felix J, Kandiah E, De Munck S, Bloch Y, van Zundert GC, Pauwels K, Dansercoer A, Novanska K, Read RJ, Bonvin AM, Vergauwen B, Verstraete K, Gutsche I, Savvides SN Structural basis of GM-CSF and IL-2 sequestration by the viral decoy receptor GIF Nat. comm. n/a 13228 n/a 2016 27819269 doi:10.1038/ncomms13228 emd_3230.map.gz Image stored as Reals 64 64 64 0 0 0 63 63 63 64 64 64 224.0 224.0 224.0 90.0 90.0 90.0 X Y Z -0.03177558 0.10385541 0.00035476 0.00756786 1
::::EMDATABANK.org::::EMD-3230::::
3.5 3.5 3.5 0.018 Negative Stain Reconstruction of GIF:IL2 complex 2 Complex of GIF:IL2 4:2 stoichiometry SEC-MALLS 0.163 protein orf virus GM-CSF/IL-2 Inhibition Factor GIF 0.101 4 tetramer NZ-2 Orf virus true Q9J5U5 IPR003184 HEK293T HEK293T pHLsec protein Ovine Interleukin-2 ovine IL-2 0.0219 monomer true 1 Ovis aries Ovine IPR009079 IPR012351 IPR000779 IPR030477 P19114 GO:0005615 GO:0002250 pHLsec HEK293T HEK293T NONE NONE LAB6 BRIGHT FIELD 294 FLOOD BEAM
Low-dose mode
KODAK SO-163 FILM 2 JEOL 1200EXII 01-OCT-2013 OTHER 100 40000 ZEISS SCAI 26 7 8 Sample was applied to the clear side of carbon on a carbon-mica interface and stained with 2 % (w/v) uranyl acetate. particle singleParticle Projection-matching RELION, VIPER each particle
final map was filtered to 25A
19 FSC 0.143, gold-standard C2 31675
E2BOXER, RELION, VIPER