2017-05-03 OBS 2015-11-04 PDBe PDBe 2015-11-18 2017-05-03
EMD-3229 is not valid anymore
Negative Stain electron microscopy of GM-CSF:GIF complex Felix J, Kandiah E, van Zundert GCP, Pauwels K, Novanska K, Verstraete K, Bloch Y, Read RJ, Vergauwen B, Gutsche I, Savvides SN immune system, ORF virus, cytokines, cytokine receptors Felix J, Kandiah E, van Zundert GCP, Pauwels K, Novanska K, Verstraete K, Bloch Y, Read RJ, Vergauwen B, Gutsche I, Savvides SN Structural basis of GM-CSF and IL-2 sequestration by the viral decoy receptor GIF to achieve immunomodulation To Be Published emd_3229.map.gz Image stored as Reals 64 64 64 0 0 0 63 63 63 64 64 64 224.0 224.0 224.0 90.0 90.0 90.0 X Y Z -0.04944067 0.10318178 0.00030788 0.00862715 1
::::EMDATABANK.org::::EMD-3229::::
3.5 3.5 3.5 0.0286 Negative Stain Reconstruction of GM-CSF:GIF complex 2 Complex of GM-CSF:GIF 0.164 protein orf virus GM-CSF/IL-2 Inhibition Factor GIF 0.101 4 tetramer NZ-2 Orf virus true Q9J5U5 IPR003184 HEK293T HEK293T pHLsec protein ovine granulocyte macrophage colony-stimulating factor ovine GM-CSF 0.0192 monomer true 1 Ovis aries Ovine IPR009079 IPR012351 IPR000773 P28773 GO:0005615 GO:0001892 GO:0006955 pHLsec HEK293T HEK293T NONE NONE LAB6 BRIGHT FIELD 294 FLOOD BEAM
Low-dose mode
KODAK SO-163 FILM 2 JEOL 1200EXII 01-OCT-2013 OTHER 100 40000 ZEISS SCAI 30 7 8 Sample was applied to the clear side of carbon on a carbon-mica interface and stained with 2 % (w/v) uranyl acetate. particle singleParticle Projection-matching RELION, VIPER each particle
final map was filtered to 25A
17 FSC 0.143, gold-standard C2 24324
E2BOXER, RELION, VIPER