HPUB OBS PDBe PDBe PDBe 2015-11-04 2015-11-18 2017-05-03 2017-05-03 Negative Stain electron microscopy of GM-CSF:GIF complex Felix J Kandiah E van Zundert GCP Pauwels K Novanska K Verstraete K Bloch Y Read RJ Vergauwen B Gutsche I Savvides SN immune system, ORF virus, cytokines, cytokine receptors Felix J Kandiah E van Zundert GCP Pauwels K Novanska K Verstraete K Bloch Y Read RJ Vergauwen B Gutsche I Savvides SN Structural basis of GM-CSF and IL-2 sequestration by the viral decoy receptor GIF to achieve immunomodulation To Be Published Complex of GM-CSF:GIF Complex of GM-CSF:GIF 2 0.164 orf virus GM-CSF/IL-2 Inhibition Factor Orf virus NZ-2 0.101 4 tetramer true HEK293T HEK293T pHLsec Q9J5U5 IPR003184 ovine granulocyte macrophage colony-stimulating factor Ovis aries Ovine 0.0192 1 monomer true HEK293T HEK293T pHLsec P28773 GO:0005615 GO:0001892 GO:0006955 IPR009079 IPR012351 IPR000773 singleParticle particle NEGATIVE
Sample was applied to the clear side of carbon on a carbon-mica interface and stained with 2 % (w/v) uranyl acetate.
 NONE OTHER JEOL 1200EXII FLOOD BEAM BRIGHT FIELD LAB6 100 2 40000.0 OTHER 294
Low-dose mode
2013-10-01 KODAK SO-163 FILM ZEISS SCAI 7 30 8.0
E2BOXER, RELION, VIPER
each particle
 OTHER 17.0 OTHER RELION, VIPER
final map was filtered to 25A
24324 emd_3229.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 64 64 64 0 0 0 64 64 64 224.0 224.0 224.0 90.0 90.0 90.0 X Y Z -0.04944067 0.10318178 0.00030788 0.00862715 3.5 3.5 3.5 0.0286 AUTHOR Negative Stain Reconstruction of GM-CSF:GIF complex
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