2016-11-02 OBS 2015-10-23 PDBe PDBe 2016-01-20 2016-11-02 EMD-8439
new deposition
CryoEM structure of the human IKK1 hexamer Polley S, Oliveira Passos D, Huang D, Biswas T, Verma IM, Lyumkis D, Ghosh G signal transduction, NFkB, inflammation, non-canonical signaling, cancer Polley S, Oliveira Passos D, Huang D, Biswas T, Verma IM, Lyumkis D, Ghosh G Structure of the IKK1 Hexamer Reveals the Basis for Its Distinct Role in NF-kappaB Signaling To Be Published emd_3211.map.gz Image stored as Reals 320 320 320 0 0 0 319 319 319 320 320 320 419.19998 419.19998 419.19998 90.0 90.0 90.0 X Y Z -0.07212129 0.13111335 -0.00032098 0.00552402 1
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1.31 1.31 1.31 0.35 reconstruction of human IKK1 hexamer 1 human IKK1 protein hexamer 0.45 protein Inhibitor of kappaB kinase 1 IKK1 0.45 Homo sapiens true hexamer Human 1 IPR011009 IPR000719 IPR002290 IPR022007 IPR017441 IPR008271 H9F0W6 cytosol & nucleus pFastBacHTa Spodoptera frugiperda Sf9 IKK1 sample (3 microliters) was applied onto a freshly plasma treated (6s, Gatan Solarus plasma cleaner) holey carbon C-flat grid (Protochips, Inc.), allowing the sample to adsorb for 30-seconds, and then plunge-freezing into liquid ethane using a manual cryo-plunger in an ambient environment of 4C. ETHANE 90 HOMEMADE PLUNGER 77 Objective lens astigmatism was corrected using Leginon software FIELD EMISSION GUN 40 BRIGHT FIELD 1100 5200 FLOOD BEAM GATAN K2 SUMMIT (4k x 4k) 2.7 0 38167 0 FEI TITAN KRIOS 20-SEP-2014 FEI TITAN KRIOS AUTOGRID HOLDER 300 22500 2792
every image is the average of 50 frames recorded by the K2 summit direct detector
 particle 2 C-flat grid, 1.2/1.3 micron holes/spacing
20mM Tris-HCl, 150mM NaCl, 5mM DTT, 2.5% Glycerol, 0.05% NP-40
7.5 singleParticle projection matching Relion, Frealign each particle 5.9 FSC 0.143, semi-independent D3 1432
all pre-processing performed within Appion suite