HPUB OBS PDBe PDBe PDBe 2015-10-23 2016-01-20 2016-11-02 2016-11-02 EMD-8438 CryoEM structure of the human IKK1 dimer, conformation 3 Polley S Oliveira Passos D Huang D Biswas T Verma IM Lyumkis D Ghosh G signal transduction, NFkB, inflammation, non-canonical signaling, cancer Polley S Oliveira Passos D Huang D Biswas T Verma IM Lyumkis D Ghosh G Structure of the IKK1 Hexamer Reveals the Basis for Its Distinct Role in NF-kappaB Signaling To Be Published human IKK1 protein human IKK1 protein dimer 1 0.15 0.15 size-exclusion chromatography Inhibitor of kappaB kinase 1 Homo sapiens Human cytosol & nucleus 0.15 0.15 1 dimer true Spodoptera frugiperda Sf9 pFastBacHTa H9F0W6 IPR011009 IPR000719 IPR002290 IPR022007 IPR017441 IPR008271 singleParticle particle 2 7.5
20mM Tris-HCl, 150mM NaCl, 5mM DTT, 2.5% Glycerol, 0.05% NP-40
C-flat grid, 1.2/1.3 micron holes/spacing
 ETHANE 90 77 HOMEMADE PLUNGER IKK1 sample (3 microliters) was applied onto a freshly plasma treated (6s, Gatan Solarus plasma cleaner) holey carbon C-flat grid (Protochips, Inc.), allowing the sample to adsorb for 30-seconds, and then plunge-freezing into liquid ethane using a manual cryo-plunger in an ambient environment of 4C. FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 2.7 1.1 5.2 22500.0 38167.0 FEI TITAN KRIOS AUTOGRID HOLDER Objective lens astigmatism was corrected using Leginon software 2014-09-20 GATAN K2 SUMMIT (4k x 4k) 2792 40
every image is the average of 50 frames recorded by the K2 summit direct detector
 0 0
all pre-processing performed within Appion suite
each particle
 OTHER 4.5 OTHER Relion, Frealign 30540 emd_3210.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 192 192 192 0 0 0 192 192 192 251.51999 251.51999 251.51999 90.0 90.0 90.0 X Y Z -0.53002071 1.2801733 0.0043608 0.06357984 1.31 1.31 1.31 0.35 AUTHOR reconstruction of human IKK1 dimer, conformation 3
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