2016-03-09 OBS 2015-10-04 PDBe PDBe 2015-11-04 2016-03-09
Mechanisms of Ribosome Stalling by SecM at Multiple Elongation Steps Zhang J, Pan XJ, Yan KG, Sun S, Gao N, Sui SF electron microscopy, single particle analysis, ribosome stalling Zhang J, Pan X, Yan K, Sun S, Gao N, Sui SF Mechanisms of ribosome stalling by SecM at multiple elongation steps. ELIFE 4 2015 26670735 doi:10.7554/eLife.09684 emd_3190.map.gz Image stored as Reals 320 320 320 0 0 0 319 319 319 320 320 320 422.40002 422.40002 422.40002 90.0 90.0 90.0 X Y Z -0.08421799 0.16016082 0.00010774 0.00801697 1
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1.32 1.32 1.32 0.03 Cryo EM reconstruction of SecM-Pro-RNC(ribosome-nascent-peptide-complex) without mask
emd_3190.tif
emd_3190_fsc.xml 1 SecM-Pro-RNC(ribosome-nascent-peptide-complex) complex without mask 2.3 ribosome-prokaryote SecM-Pro-RNC without mask SecM-Pro-stalled RNC without mask 2.3 ALL Escherichia coli true pET21 Escherichia coli str. K-12 substr. MG1655 Blot for 1.5 seconds before plunging ETHANE 100 FEI VITROBOT MARK IV 277.15 BRIGHT FIELD FEI TITAN KRIOS AUTOGRID HOLDER 2.7 FLOOD BEAM 37878 08-MAY-2014 22500.0 1000 16 FIELD EMISSION GUN FEI TITAN KRIOS GATAN K2 (4k x 4k) 3500 300 16 GATAN K2 (4k x 4k) 37878 FLOOD BEAM FEI TITAN KRIOS 1000 300 BRIGHT FIELD 3500 FIELD EMISSION GUN 2.7 22500.0 FEI TITAN KRIOS AUTOGRID HOLDER 16-JUN-2014 37878 FEI TITAN KRIOS FIELD EMISSION GUN BRIGHT FIELD FLOOD BEAM 22500.0 GATAN K2 (4k x 4k) 1000 300 2.7 30-AUG-2014 16 3500 FEI TITAN KRIOS AUTOGRID HOLDER 3908 4
Every image is the average of 14 frames recorded by the direct electron detector
 Chimera, EMFit, Coot flexible RECIPROCAL 4V7T A B particle
20mM HEPES, 50mM KOAc, 6mM Mg[OAc]2, 1mM DTT, 500 ug/ml chloramphenicol,0.05% Nikkol,0.5% pill/ml Complete EDTA-free Protease inhibitor cocktail,0.1 U/ml RNasin and 125mM sucrose
7.0 singleParticle reference-based reconstruction RELION CTFFIND 3.32 FSC 0.143, gold-standard C1 60354