HPUB OBS PDBe PDBe PDBe 2015-10-04 2015-11-04 2016-03-09 2016-03-09 Mechanisms of Ribosome Stalling by SecM at Multiple Elongation Steps Zhang J Pan XJ Yan KG Sun S Gao N Sui SF electron microscopy, single particle analysis, ribosome stalling Zhang J Pan X Yan K Sun S Gao N Sui SF Mechanisms of ribosome stalling by SecM at multiple elongation steps. ELIFE 4 2015 26670735 doi:10.7554/eLife.09684 SecM-Gly-RNC(ribosome-nascent-peptide-complex) complex with 50S mask SecM-Gly-RNC(ribosome-nascent-peptide-complex) complex with 50S mask 1 2.3 SecM-Gly-RNC with 50S mask true Escherichia coli Escherichia coli str. K-12 substr. MG1655 pET21 2.3 ribosome-prokaryote: ALL singleParticle particle 7.0
20mM HEPES, 50mM KOAc, 6mM Mg[OAc]2, 1mM DTT, 500 ug/ml chloramphenicol,0.05% Nikkol,0.5% pill/ml Complete EDTA-free Protease inhibitor cocktail,0.1 U/ml RNasin and 125mM sucrose
 ETHANE 100 277.15 FEI VITROBOT MARK IV Blot for 1.5 seconds before plunging FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 2.7 1.0 3.5 22500.0 37878.0 FEI TITAN KRIOS AUTOGRID HOLDER 2014-05-08 GATAN K2 (4k x 4k) 4 3908 16
Every image is the average of 14 frames recorded by the direct electron detector
 FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 2.7 1.0 3.5 22500.0 FEI TITAN KRIOS AUTOGRID HOLDER 2014-06-16 GATAN K2 (4k x 4k) 4 3908 16
Every image is the average of 14 frames recorded by the direct electron detector
 FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 2.7 1.0 3.5 22500.0 FEI TITAN KRIOS AUTOGRID HOLDER 2014-08-30 GATAN K2 (4k x 4k) 4 3908 16
Every image is the average of 14 frames recorded by the direct electron detector
CTFFIND
 OTHER 3.64 OTHER RELION 60354 emd_3188.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 320 320 320 0 0 0 320 320 320 422.40002 422.40002 422.40002 90.0 90.0 90.0 X Y Z -0.09889516 0.18002944 0.00023492 0.00902666 1.32 1.32 1.32 0.03 AUTHOR Cryo EM reconstruction of SecM-Gly-RNC(ribosome-nascent-peptide-complex) with 50S mask
::::EMDATABANK.org::::EMD-3188::::
 4V7T B FLEXIBLE FIT Chimera, EMFit, Coot RECIPROCAL
emd_3188.tif
emd_3188_msk.map emd_3188_msk.map 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 320 320 320 0 0 0 320 320 320 422.40002 422.40002 422.40002 90.0 90.0 90.0 X Y Z 0.0 1.0 0.07364544 0.25647718 1.32 1.32 1.32 This mask represent the large subunit with P/P-site tRNA
::::EMDATABANK.org::::
 emd_3188_fsc.xml