Structure of a cross-beta amyloid fibril from IGSNVVTWYQQL peptide of AL-DIA immunoglobulin light chain by cryo-EM and fiber diffraction

2017-02-01 OBS 2015-08-13 PDBe PDBe 2015-09-16 2017-02-01 2017-02-01

Data reprocessed

Structure of a cross-beta amyloid fibril from IGSNVVTWYQQL peptide of AL-DIA immunoglobulin light chain by cryo-EM and fiber diffraction Schmidt A, Annamalai K, Schmidt M, Grigorieff N, Fandrich M AL amyloidosis, cryo electron microscopy, steric zipper, three dimensional reconstruction Schmidt A, Annamalai K, Schmidt M, Grigorieff N, Fandrich M Structural hierarchy, polymorphism and zipper-like packing of amyloid fibrils from an immunoglobulin light chain fragment To Be Published emd_3127.map.gz Image stored as Reals 30 30 40 -15 -15 -20 14 14 19 30 30 40 253.19998 253.19998 337.59998 90.0 90.0 90.0 X Y Z -2020.42968750 8514.20019531 695.82000732 2629.44335938 1

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8.44 8.44 8.44 0.48 Reconstruction morphology 2 of a left handed amyloid-like fibril of the IGSNVVTWYQQL fragment of an immunoglobulin light chain

emd_3127.png emd_3127_fsc.xml 1 Amyloidogenic Fragment IGSNVVTWYQQL of Immunoglobulin Light Chain of Human AL Patient

The sample forms long amyloid-like fibrils.

protein Immunoglobulin Light Chain Homo sapiens false Human Plasma cell Blood extracellular Incubation of 0.014 mg/ml fibril solution on glow discharged holey carbon grid for 30 seconds and backside blotting for 4 seconds before plunging. ETHANE 50 GATAN CRYOPLUNGE 3 100 FIELD EMISSION GUN 25 BRIGHT FIELD 1000 5000 FLOOD BEAM FEI FALCON I (4k x 4k) 2.0 66350.7 100 FEI TECNAI F20 12-NOV-2012 GATAN LIQUID NITROGEN 200 50000 5 16 4.69 0.824 C2 LEFT HANDED filament 5.0 C-flat 1.2/1.3-2C 400 mesh grids, glow discharged 8.0

50mM Tris-HCL

helical Projection matching and Fourier inversion Frealix Defocus estimated for each helical subunit

Final map was calculated from five single fibril reconstructions.

28.5 FSC 0.143, semi-independent