Structure of a cross-beta amyloid fibril from IGSNVVTWYQQL peptide of AL-DIA immunoglobulin light chain by cryo-EM and fiber diffraction

REL OBS PDBe PDBe PDBe 2015-08-13 2015-09-16 2017-02-01 2017-02-01 2017-02-01 Structure of a cross-beta amyloid fibril from IGSNVVTWYQQL peptide of AL-DIA immunoglobulin light chain by cryo-EM and fiber diffraction Schmidt A Annamalai K Schmidt M Grigorieff N Fandrich M AL amyloidosis, cryo electron microscopy, steric zipper, three dimensional reconstruction Schmidt A Annamalai K Schmidt M Grigorieff N Fandrich M Structural hierarchy, polymorphism and zipper-like packing of amyloid fibrils from an immunoglobulin light chain fragment To Be Published Amyloidogenic Fragment IGSNVVTWYQQL of Immunoglobulin Light Chain of Human AL Patient Amyloidogenic Fragment IGSNVVTWYQQL of Immunoglobulin Light Chain of Human AL Patient

The sample forms long amyloid-like fibrils.

1 Immunoglobulin Light Chain Homo sapiens Human Blood Plasma cell extracellular false helical filament 5.0 8.0

50mM Tris-HCL

C-flat 1.2/1.3-2C 400 mesh grids, glow discharged

ETHANE 50 100 GATAN CRYOPLUNGE 3 Incubation of 0.014 mg/ml fibril solution on glow discharged holey carbon grid for 30 seconds and backside blotting for 4 seconds before plunging. FEI TECNAI F20 FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 200 2.0 1.0 5.0 50000.0 66350.69999999999709 GATAN LIQUID NITROGEN 100 2012-11-12 FEI FALCON I (4k x 4k) 5 25 16.0 4.69 0.824 C2 OTHER 28.5 OTHER Frealix

Final map was calculated from five single fibril reconstructions.

Defocus estimated for each helical subunit

emd_3127.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 30 30 40 -15 -15 -20 30 30 40 253.19998 253.19998 337.59998 90.0 90.0 90.0 X Y Z -2020.4296875 8514.20019531 695.82000732 2629.44335938 8.44 8.44 8.44 0.48 AUTHOR Reconstruction morphology 2 of a left handed amyloid-like fibril of the IGSNVVTWYQQL fragment of an immunoglobulin light chain

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emd_3127.png emd_3127_fsc.xml