Cryo-EM structure of Patched1 (V1084A mutant) in lipid nanodisc

2021-09-22 OBS PDBj PDBj PDBj 2021-01-25 2021-06-23 2021-06-23 2021-09-22 2021-09-22 2021-09-22 EMD-31754 National Natural Science Foundation of China (NSFC) 31570748 China National Natural Science Foundation of China (NSFC) U1632127 China Chinese Academy of Sciences XDB37020204 China Chinese Academy of Sciences QYZDB-SSW-SMC037 China Cryo-EM structure of Patched1 (V1084A mutant) in lipid nanodisc Luo Y Zhang X Wan G Wang Q Zhao Y Cong Y Qu Q Li D Luo Y Wan G Zhang X Zhou X Wang Q Fan J Cai H Ma L Wu H Qu Q Cong Y Zhao Y Li D Cryo-EM study of patched in lipid nanodisc suggests a structural basis for its clustering in caveolae. Structure UK 2021 34174188 doi:10.1016/j.str.2021.06.004 0969-2126 2005 STRUE6 EMD-30927 associated EM volume

7dzp FULLOVERLAP Ptc1 in lipid nanodisc Ptc1 in lipid nanodisc 0 1 Mus musculus Homo sapiens 160 Protein patched homolog 1,Protein patched homolog 1 Mus musculus 0.121609555 1 Homo sapiens LEVO MGSASAGNAAGALGRQAGGGRRRRTGGPHRAAPDRDYLHRPSYCDAAFALEQISKGKATGRKAPLWLRAKFQRLLFKLGC YIQKNCGKFLVVGLLIFGAFAVGLKAANLETNVEELWVEVGGRVSRELNYTRQKIGEEAMFNPQLMIQTPKEEGANVLTT EALLQHLDSALQASRVHVYMYNRQWKLEHLCYKSGELITETGYMDQIIEYLYPCLIITPLDCFWEGAKLQSGTAYLLGKP PLRWTNFDPLEFLEELKKINYQVDSWEEMLNKAEVGHGYMDRPCLNPADPDCPATAPNKNSTKPLDVALVLNGGCQGLSR KYMHWQEELIVGGTVKNATGKLVSAHALQTMFQLMTPKQMYEHFRGYDYVSHINWNEDRAAAILEAWQRTYVEVVHQSVA PNSTQKVLPFTTTTLDDILKSFSDVSVIRVASGYLLMLAYACLTMLRWDCSKSQGAVGLAGVLLVALSVAAGLGLCSLIG ISFNAATTQVLPFLALGVGVDDVFLLAHAFSETGQNKRIPFEDRTGECLKRTGASVALTSISNVTAFFMAALIPIPALRA FSLQAAVVVVFNFAMVLLIFPAILSMDLYRREDRRLDIFCCFTSPCVSRVIQVEPQEPPCTKWTLSSFAEKHYAPFLLKP KAKVVVILLFLGLLGVSLYGTTRVRDGLDLTDIVPRETREYDFIAAQFKYFSFYNMYIVTQKADYPNIQHLLYDLHKSFS NVKYVMLEENKQLPQMWLHYFRDWLQGLQDAFDSDWETGRIMPNNYKNGSDDGVLAYKLLVQTGSRDKPIDISQLTKQRL VDADGIINPSAFYIYLTAWVSNDPVAYAASQANIRPHRPEWVHDKADYMPETRLRIPAAEPIEYAQFPFYLNGLRDTSDF VEAIEKVRVICNNYTSLGLSSYPNGYPFLFWEQYISLRHWLLLSISVVLACTFLVCAVFLLNPWTAGIIVMVLALMTVEL FGMMGLIGIKLSAVPVVILIASVGIGVEFTAHVALAFLTAIGDKNHRAMLALEHMFAPVLDGAVSTLLGVLMLAGSEFDF IVRYFFAVLAILTVLGVLNGLVLLPVLLSFFGPCPEVSPANGTLEVLFQG 2-acetamido-2-deoxy-beta-D-glucopyranose 0.000221208 3 NAG CHOLESTEROL 0.000386654 1 CLR singleParticle particle 8.5 7.5 150.0 NaCl sodium chloride 20.0 HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) 0.5 CaCl2 calsium chloride 0.1 EDTA Ethylenediaminetetraacetic acid 0.2 TCEP tris(2-carboxyethyl)phosphine ETHANE 100 277 FEI VITROBOT MARK IV FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 70.0 2.7 -1.5 -1.1 -2.2 -3.0 120000.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN GATAN K3 (6k x 4k) 5760 4092 1 5140 2.23 60.0 1 3311691 Gctf BACK PROJECTION 4.0 FSC 0.143 CUT-OFF RELION 3.1 165326 RANDOM ASSIGNMENT RELION 3.1 PROJECTION MATCHING 1 RELION 3.1 4 63736.0 RELION 3.1 emd_30927.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 264 264 264 0 0 0 264 264 264 216.48 216.48 216.48 90.0 90.0 90.0 X Y Z -0.0476257 0.08210765 0.00014335365 0.0019920191 0.82 0.82 0.82 0.0118 AUTHOR ::::EMDATABANK.org::::EMD-30927:::: OTHER REAL 57.8 emd_30927_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 264 264 264 0 0 0 264 264 264 216.48 216.48 216.48 90.0 90.0 90.0 X Y Z -0.020777036 0.03572568 -1.3511211e-05 0.002386524 0.82 0.82 0.82 AUTHOR ::::EMDATABANK.org::::EMD-30927:::: emd_30927_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 264 264 264 0 0 0 264 264 264 216.48 216.48 216.48 90.0 90.0 90.0 X Y Z -0.020042505 0.036703374 -1.3219466e-05 0.0023726046 0.82 0.82 0.82 AUTHOR ::::EMDATABANK.org::::EMD-30927::::