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    <admin>
        <current_status>
            <date>2021-06-23</date>
            <code>OBS</code>
            <processing_site>PDBj</processing_site>
        </current_status>
        <sites>
            <deposition>PDBj</deposition>
            <last_processing>PDBj</last_processing>
        </sites>
        <key_dates>
            <deposition>2020-12-03</deposition>
            <header_release>2020-12-30</header_release>
            <map_release>2020-12-30</map_release>
            <obsolete>2021-06-23</obsolete>
            <update>2021-06-23</update>
        </key_dates>
        <title>Unliganded EGFR averaged cluster 21</title>
        <authors_list>
            <author>Purba ER</author>
            <author>Saita EI</author>
            <author>Akhouri RR</author>
            <author>Ofverstedt LG</author>
            <author>Wilken G</author>
            <author>Skoglund U</author>
            <author>Maruyama IN</author>
        </authors_list>
    </admin>
    <crossreferences>
        <citation_list>
            <primary_citation>
                <journal_citation published="false">
                    <author order="1">Purba ER</author>
                    <author order="2">Saita EI</author>
                    <author order="3">Akhouri RR</author>
                    <author order="4">Ofverstedt LG</author>
                    <author order="5">Wilken G</author>
                    <author order="6">Skoglund U</author>
                    <author order="7">Maruyama IN</author>
                    <title>Conformation flexibility transitions of the epidermal growth factor receptor dimer upon activation</title>
                    <journal_abbreviation>To Be Published</journal_abbreviation>
                    <external_references type="CSD">0353</external_references>
                </journal_citation>
            </primary_citation>
        </citation_list>
        <emdb_list>
            <emdb_reference>
                <emdb_id>EMD-30735</emdb_id>
                <relationship>
                    <other>other EM volume</other>
                </relationship>
            </emdb_reference>
            <emdb_reference>
                <emdb_id>EMD-30736</emdb_id>
                <relationship>
                    <other>associated EM volume</other>
                </relationship>
            </emdb_reference>
        </emdb_list>
    </crossreferences>
    <sample>
        <name>Unliganded EGFR averaged cluster 21</name>
        <supramolecule_list>
            <complex_supramolecule supramolecule_id="1">
                <name>Unliganded EGFR averaged cluster 21</name>
                <parent>0</parent>
                <natural_source database="NCBI">
                    <organism ncbi="9606">Homo sapiens</organism>
                    <strain>DH5a</strain>
                    <cellular_location>cellular membrane</cellular_location>
                </natural_source>
                <molecular_weight>
                    <theoretical units="MDa">0.3</theoretical>
                </molecular_weight>
            </complex_supramolecule>
        </supramolecule_list>
    </sample>
    <structure_determination_list>
        <structure_determination structure_determination_id="1">
            <method>tomography</method>
            <aggregation_state>particle</aggregation_state>
            <specimen_preparation_list>
                <tomography_preparation preparation_id="1">
                    <concentration units="mg/mL">0.2</concentration>
                    <buffer>
                        <ph>8.0</ph>
                        <component>
                            <concentration units="mM">20.0</concentration>
                            <name>Tris-HCL</name>
                        </component>
                        <component>
                            <concentration units="mM">200.0</concentration>
                            <name>Sodium chloride</name>
                        </component>
                        <component>
                            <concentration units="mM">0.2</concentration>
                            <name>Dodecyl maltocide</name>
                        </component>
                    </buffer>
                    <grid>
                        <model>Quantifoil R1.2/1.3</model>
                        <material>COPPER</material>
                        <mesh>200</mesh>
                        <support_film film_type_id="1">
                            <film_material>CARBON</film_material>
                            <film_topology>HOLEY</film_topology>
                            <film_thickness units="nm">120.0</film_thickness>
                        </support_film>
                    </grid>
                    <vitrification>
                        <cryogen_name>ETHANE</cryogen_name>
                        <chamber_humidity units="percentage">85</chamber_humidity>
                        <chamber_temperature units="K">277.0</chamber_temperature>
                        <instrument>FEI VITROBOT MARK IV</instrument>
                    </vitrification>
                    <details>the sample was monodisperse</details>
                    <cryo_protectant>No</cryo_protectant>
                    <sectioning>
                        <other_sectioning>NO SECTIONING</other_sectioning>
                    </sectioning>
                </tomography_preparation>
            </specimen_preparation_list>
            <microscopy_list>
                <tomography_microscopy microscopy_id="1">
                    <microscope>FEI TITAN KRIOS</microscope>
                    <illumination_mode>OTHER</illumination_mode>
                    <imaging_mode>BRIGHT FIELD</imaging_mode>
                    <electron_source>FIELD EMISSION GUN</electron_source>
                    <acceleration_voltage units="kV">300</acceleration_voltage>
                    <c2_aperture_diameter units="µm">100.0</c2_aperture_diameter>
                    <nominal_cs units="mm">2.7</nominal_cs>
                    <nominal_defocus_min units="µm">1.5</nominal_defocus_min>
                    <calibrated_defocus_min units="µm">1.0</calibrated_defocus_min>
                    <calibrated_defocus_max units="µm">1.5</calibrated_defocus_max>
                    <nominal_magnification>37000.0</nominal_magnification>
                    <calibrated_magnification>37000.0</calibrated_magnification>
                    <specimen_holder_model>FEI TITAN KRIOS AUTOGRID HOLDER</specimen_holder_model>
                    <cooling_holder_cryogen>NITROGEN</cooling_holder_cryogen>
                    <temperature>
                        <temperature_min units="K">70.0</temperature_min>
                        <temperature_max units="K">70.0</temperature_max>
                    </temperature>
                    <alignment_procedure>
                        <basic />
                    </alignment_procedure>
                    <image_recording_list>
                        <image_recording image_recording_id="1">
                            <film_or_detector_model>FEI FALCON II (4k x 4k)</film_or_detector_model>
                            <detector_mode>INTEGRATING</detector_mode>
                            <number_grids_imaged>1</number_grids_imaged>
                            <number_real_images>134</number_real_images>
                            <average_exposure_time units="s">1.8</average_exposure_time>
                            <average_electron_dose_per_image units="e/Å^2">90.0</average_electron_dose_per_image>
                        </image_recording>
                    </image_recording_list>
                </tomography_microscopy>
            </microscopy_list>
            <tomography_processing image_processing_id="1">
                <image_recording_id>1</image_recording_id>
                <final_reconstruction>
                    <algorithm>BACK PROJECTION</algorithm>
                    <resolution res_type="BY AUTHOR" units="Å">15.0</resolution>
                    <resolution_method>OTHER</resolution_method>
                    <number_images_used>141</number_images_used>
                </final_reconstruction>
            </tomography_processing>
        </structure_determination>
    </structure_determination_list>
    <map format="CCP4" size_kbytes="5325">
        <file>emd_30736.map.gz</file>
        <symmetry>
            <space_group>1</space_group>
        </symmetry>
        <data_type>IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)</data_type>
        <dimensions>
            <col>110</col>
            <row>110</row>
            <sec>110</sec>
        </dimensions>
        <origin>
            <col>0</col>
            <row>0</row>
            <sec>0</sec>
        </origin>
        <spacing>
            <x>110</x>
            <y>110</y>
            <z>110</z>
        </spacing>
        <cell>
            <a units="Å">248.38002</a>
            <b units="Å">248.38002</b>
            <c units="Å">248.38002</c>
            <alpha units="deg">90.0</alpha>
            <beta units="deg">90.0</beta>
            <gamma units="deg">90.0</gamma>
        </cell>
        <axis_order>
            <fast>X</fast>
            <medium>Y</medium>
            <slow>Z</slow>
        </axis_order>
        <statistics>
            <minimum>0.16451259</minimum>
            <maximum>0.7890019</maximum>
            <average>0.3705062</average>
            <std>0.07170954</std>
        </statistics>
        <pixel_spacing>
            <x units="Å">2.2580001</x>
            <y units="Å">2.2580001</y>
            <z units="Å">2.2580001</z>
        </pixel_spacing>
        <contour_list>
            <contour primary="true">
                <source>AUTHOR</source>
            </contour>
        </contour_list>
        <label>::::EMDATABANK.org::::EMD-30736::::</label>
    </map>
    <interpretation>
        <modelling_list>
            <modelling>
                <initial_model>
                    <access_code>1NQL</access_code>
                    <chain>
                        <chain_id>A</chain_id>
                        <residue_range>3-614</residue_range>
                    </chain>
                </initial_model>
                <initial_model>
                    <access_code>2M0B</access_code>
                    <chain>
                        <chain_id>A</chain_id>
                        <residue_range>634-677</residue_range>
                    </chain>
                </initial_model>
                <initial_model>
                    <access_code>2M0B</access_code>
                    <chain>
                        <chain_id>B</chain_id>
                        <residue_range>634-677</residue_range>
                    </chain>
                </initial_model>
                <initial_model>
                    <access_code>3GT8</access_code>
                    <chain>
                        <chain_id>A</chain_id>
                        <residue_range>676-990</residue_range>
                    </chain>
                </initial_model>
                <initial_model>
                    <access_code>3GT8</access_code>
                    <chain>
                        <chain_id>B</chain_id>
                        <residue_range>677-989</residue_range>
                    </chain>
                </initial_model>
                <refinement_protocol>FLEXIBLE FIT</refinement_protocol>
                <target_criteria>Correlation coefficient</target_criteria>
                <refinement_space>REAL</refinement_space>
            </modelling>
        </modelling_list>
    </interpretation>
</emd>