2016-02-10 OBS 2015-06-22 PDBe PDBe 2015-08-12 2016-02-10 EMD-3233
Data was reprocessed.
Phase plate structure of Peroxiredoxin-3 dodecamers Khoshouei M, Radjainia M, Abrishami V, Phillips AJ, Gerrard JA, Mitra AK, Plitzko JM, Baumeister W, Danev R Volta phase plate, peroxiredoxin, peroxidase, antioxidant Khoshouei M, Radjainia M, Abrishami V, Phillips AJ, Gerrard JA, Mitra AK, Plitzko JM, Baumeister W, Danev R Volta phase plate cryo-EM of the small protein complex Prx3 NAT.COMMUN. emd_3060.map.gz Image stored as Reals 152 152 152 0 0 0 151 151 151 152 152 152 205.2 205.2 205.2 90.0 90.0 90.0 X Y Z -0.07650846 0.11458807 0.00075023 0.00704310 1
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1.35 1.35 1.35 0.024 Single-particle reconstruction of Prx3 dodecamers from Volta Phase Contrast Images emd_3060_fsc.xml 1 Peroxiredoxin-3 dodecamers 12 0.257
The sample was monodisperse
SEC and MS 0.257 protein Human Peroxiredoxin-3 Prx3 0.257 0.257 Homo sapiens true dodecamer Human 1 P30048 Mitochondria matrix Escherichia coli ETHANE FEI VITROBOT MARK III FIELD EMISSION GUN BRIGHT FIELD FLOOD BEAM GATAN K2 (4k x 4k) FEI TITAN KRIOS 26-FEB-2015 FEI TITAN KRIOS AUTOGRID HOLDER 300 44 particle 1 Quantifoil R2/2
20mM Hepes pH8, 150m NaCl
8 singleParticle Relion 7.3 FSC 0.143, gold-standard D6 7000