2016-08-24 OBS 2015-06-09 PDBe PDBe 2015-07-15 2016-08-24
Map recalculated using direct electron detector
RNC-Ski238-complex in the "tethered" state Schmidt C, Kowalinski E, Shanmuganathan V, Defenouillere Q, Braunger K, Pech M, Berninghausen O, Fromont-Racine M, Jacquier A, Conti E, Becker T, Beckmann R 3'-5' mRNA decay, non-stop mRNA decay, ribosome stalling Schmidt C, Kowalinski E, Shanmuganathan V, Defenouillere Q, Braunger K, Pech M, Berninghausen O, Fromont-Racine M, Jacquier A, Conti E, Becker T, Beckmann R he Ski complex binds 80S ribosomes for 3- to 5- decay of mRNA To Be Published emd_3042.map.gz Image stored as Reals 370 370 370 -185 -185 -184 184 184 185 370 370 370 492.1 492.1 492.1 90.0 90.0 90.0 X Y Z -0.74449033 1.84592807 -0.00238067 0.15111394 1
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1.33 1.33 1.33 0.252 RNC-Ski238 complex in the "tethered" state 4 RNC-Ski238-complex in the "tethered" state One ribosome binds to one Ski complex (consisting of Ski2, Ski3 and two copies of Ski8) 3.4 ribosome-eukaryote 80S ribosome ALL Saccharomyces cerevisiae Baker's Yeast false protein Antiviral helicase SKI2 Ski2 0.146 true Saccharomyces cerevisiae Monomer 1 Baker's Yeast P35207 Trichoplusia ni High five pFastBac protein Superkiller protein 3 Ski3 0.164 Saccharomyces cerevisiae 1 Baker's Yeast monomer true P17883 pFastBac Trichoplusia ni High five protein Antiviral protein SKI8 Ski8 0.044 true Dimer 2 Saccharomyces cerevisiae Baker's Yeast Q02793 high five pFastBac Trichoplusia ni Blot for 3 seconds before plunging ETHANE 100 FEI VITROBOT MARK IV FIELD EMISSION GUN 25 BRIGHT FIELD 910 4330 FLOOD BEAM TVIPS TEMCAM-F816 (8k x 8k) 2.7 FEI TITAN KRIOS 30-JUN-2014 FEI TITAN KRIOS AUTOGRID HOLDER 300 8425 16 Cryo-EM particle Quantifoil Grids with 2 nm carbon on top
20 mM Tris pH 7.0, 150 mM potassium acetate (KOAc), 10 mM magnesium acetate (Mg(OAc)2), 1 mM dithiothreitol (DTT) and 0.01 mg/ml cycloheximide.
7.0 singleParticle projectionj matching (SPIDER SPIDER on volumes (SPIDER) 8.54 FSC 0.5, semi-independent C1 30508
This is a data subset after computational sorting with SPIDER