HPUB OBS PDBe PDBe PDBe 2015-06-09 2015-07-15 2016-08-24 2016-08-24 RNC-Ski238-complex in the "tethered" state Schmidt C Kowalinski E Shanmuganathan V Defenouillere Q Braunger K Pech M Berninghausen O Fromont-Racine M Jacquier A Conti E Becker T Beckmann R 3'-5' mRNA decay, non-stop mRNA decay, ribosome stalling Schmidt C Kowalinski E Shanmuganathan V Defenouillere Q Braunger K Pech M Berninghausen O Fromont-Racine M Jacquier A Conti E Becker T Beckmann R he Ski complex binds 80S ribosomes for 3- to 5- decay of mRNA To Be Published RNC-Ski238-complex in the "tethered" state RNC-Ski238-complex in the "tethered" state One ribosome binds to one Ski complex (consisting of Ski2, Ski3 and two copies of Ski8) 4 3.4 80S ribosome false Saccharomyces cerevisiae Baker's Yeast ribosome-eukaryote: ALL Antiviral helicase SKI2 Saccharomyces cerevisiae Baker's Yeast 0.146 1 Monomer true Trichoplusia ni High five pFastBac P35207 Superkiller protein 3 Saccharomyces cerevisiae Baker's Yeast 0.164 1 monomer true Trichoplusia ni High five pFastBac P17883 Antiviral protein SKI8 Saccharomyces cerevisiae Baker's Yeast 0.044 2 Dimer true Trichoplusia ni high five pFastBac Q02793 singleParticle particle 7.0
20 mM Tris pH 7.0, 150 mM potassium acetate (KOAc), 10 mM magnesium acetate (Mg(OAc)2), 1 mM dithiothreitol (DTT) and 0.01 mg/ml cycloheximide.
 NEGATIVE
Cryo-EM
Quantifoil Grids with 2 nm carbon on top
 ETHANE 100 FEI VITROBOT MARK IV Blot for 3 seconds before plunging FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 2.7 0.91 4.33 FEI TITAN KRIOS AUTOGRID HOLDER 2014-06-30 TVIPS TEMCAM-F816 (8k x 8k) 8425 25 16.0
This is a data subset after computational sorting with SPIDER
on volumes (SPIDER)
 OTHER 8.54 OTHER SPIDER 30508 emd_3042.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 370 370 370 -185 -185 -184 370 370 370 492.1 492.1 492.1 90.0 90.0 90.0 X Y Z -0.74449033 1.84592807 -0.00238067 0.15111394 1.33 1.33 1.33 0.252 AUTHOR RNC-Ski238 complex in the "tethered" state
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