REL OBS PDBe PDBe PDBe 2015-05-28 2015-08-12 2017-02-01 2017-02-01 2017-02-01 Single particle electron microscopy of the type 6 secretion system complex TssA Zoued A Durand E Spinelli S Brunet YR Douzi B Bebeacua C Legrand P Guzzo M Journet L Mignot T Cambillau C Cascales E Single particle, EM, TssA, type 6 secretion system Zoued A Durand E Spinelli S Brunet YR Douzi B Bebeacua C Legrand P Guzzo M Journet L Mignot T Cambillau C Cascales E Caught in the act: TssA primes and leads polymerization of the Type VI secretion tail tube and sheath To Be Published EMD-3030 FULLOVERLAP TssA complex full length TssA complex full length dodecamer 1 0.792 TssA Escherichia coli BL21 0.792
The complex appears as a dodecamer but presents 6-fold symmetry.
1 Dodecamer true Escherichia coli BL21 pETG-20A singleParticle particle 0.05 8.0
Tris-HCl 20 mM, NaCl 150 mM.
 NEGATIVE
The sample was incubated on a previously glow-discharged carbon grid at a concentration of 0.05 mgml-1 for 1 minute. Excess solution was blotted away and the grid was then stained with 2% uranyl acetate and left to air dry after the blotting away excess staining.
Carbon coated grids.
 NONE OTHER FEI TECNAI SPIRIT FLOOD BEAM BRIGHT FIELD LAB6 120 2.2 60000.0 60000.0 SIDE ENTRY, EUCENTRIC 2012-11-01 GENERIC CCD 100 10
Particles were selected using the program boxer from the EMAN2 package, extracted into boxes and combined into a dataset. The dataset was pre-treated using the SPIDER package and submitted to maximum likelihood (ML) classification and alignment using the Xmipp package. The aligned datasets were then classified by principal component analysis (PCA) using SPIDER into classes with approximately 10 members per class. The initial models were built from class averages selected as a side-views imposing 6-fold symmetry. Due to artefacts resulting from the excess numbers of top-view images, the dataset was reduced to their side-images components during refinement. The initial model was then refined by three-dimensional ML refinement over the reduced dataset imposing C6 symmetry.
OTHER 29.0 OTHER Xmipp, Spider 7000 500 emd_3029.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 64 64 64 5 5 5 64 64 64 451.84 451.84 451.84 90.0 90.0 90.0 X Y Z -0.04381436 0.21201333 0.00453571 0.02226595 7.06 7.06 7.06 0.01 AUTHOR C6 reconstruction of the TssA complex
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