2024-11-20 OBS RCSB RCSB RCSB 2022-11-08 2022-11-23 2022-11-23 2024-11-20 2024-11-20 National Institutes of Health/National Cancer Institute (NIH/NCI) R01CA193578 United States National Institutes of Health/National Cancer Institute (NIH/NCI) R01CA227261 United States National Institutes of Health/National Cancer Institute (NIH/NCI) R01CA219700 United States Wild type P53 dimer structure from human cancer cells Solares M Kelly DF cancer, tumor suppressor, cell cycle, apoptosis, DNA repair, ANTITUMOR PROTEIN Solares MJ Jonaid GM Luqiu WY Berry S Khadela J Liang Y Evans MC Pridham KJ Dearnaley WJ Sheng Z Kelly DF RETRACTION: High-Resolution Imaging of Human Cancer Proteins Using Microprocessor Materials Chembiochem GE 2024 39497285 doi:10.1002/cbic.202400798 1439-7633 Solares MJ Jonaid GM Luqiu WY Berry S Khadela J Liang Y Evans MC Pridham KJ Dearnaley WJ Sheng Z Kelly DF High-Resolution Imaging of Human Cancer Proteins Using Microprocessor Materials. Chembiochem GE 23 e202200310 e202200310 2022 35789183 doi:10.1002/cbic.202200310 1439-7633 8f2h FULLOVERLAP P53 dimer isolated from U87-MG cells P53 dimer isolated from U87-MG cells 0 1
Native protein, wild-type with no tags
Homo sapiens Brain Brain tumor Cellular tumor antigen p53 Homo sapiens brain tumor brain 0.043711176 2 LEVO MEEPQSDPSVEPPLSQETFSDLWKLLPENNVLSPLPSQAMDDLMLSPDDIEQWFTEDPGPDEAPRMPEAAPPVAPAPAAP TPAAPAPAPSWPLSSSVPSQKTYQGSYGFRLGFLHSGTAKSVTCTYSPALNKMFCQLAKTCPVQLWVDSTPPPGTRVRAM AIYKQSQHMTEVVRRCPHHERCSDSDGLAPPQHLIRVEGNLRVEYLDDRNTFRHSVVVPYEPPEVGSDCTTIHYNYMCNS SCMGGMNRRPILTIITLEDSSGNLLGRNSFEVRVCACPGRDRRTEEENLRKKGEPHHELPPGSTKRALPNNTSSSPQPKK KPLDGEYFTLQIRGRERFEMFRELNEALELKDAQAGKEPGGSRAHSSHLKSKKGQSTSRHKKLMFKTEGPDSD P04637 singleParticle particle 0.2 7.5
20 mM HEPES (pH 7.5), 140 mM NaCl, 2 mM MgCl2, 2 mM CaCl2, 5 mM imidazole
 Homemade SILICON NITRIDE
Silicon nitride chips coated with Ni-NTA layers prior to sample application
 ETHANE 90 298 FEI VITROBOT MARK III
The microchip samples were loaded into a FEI Mark III Vitrobot and flash-frozen into liquid ethane..
Sample was placed on Silicon nitride chips coated with Ni-NTA layers
 TFS TALOS F200C FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 200 100.0 2.7 1.0 5.0 142000.0 GATAN 626 SINGLE TILT LIQUID NITROGEN CRYO TRANSFER HOLDER NITROGEN FEI CETA (4k x 4k) 10 300 1.0 5.0 1 8000 1 C2 BACK PROJECTION 4.2 FSC 0.143 CUT-OFF RELION 8000 ANGULAR RECONSTITUTION RELION ANGULAR RECONSTITUTION RELION 1 8000.0 RELION emd_28816.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 91 97 80 -45 -48 -39 91 97 80 100.1 106.700005 88.0 90.0 90.0 90.0 X Y Z -4.2792163 8.506843 0.000000000015819 1.0 1.1 1.1 1.1 0.2 AUTHOR P53 dimer structure FLEXIBLE FIT REAL 50.0 emd_28816_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 87 97 76 -45 -48 -39 87 97 76 95.700005 106.700005 83.6 90.0 90.0 90.0 X Y Z -8.706413 10.457101 -0.000000000013625 1.0 1.1 1.1 1.1 AUTHOR Half Map 2 emd_28816_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 87 97 76 -45 -48 -39 87 97 76 95.700005 106.700005 83.6 90.0 90.0 90.0 X Y Z -6.3088307 9.377618999999999 0.000000000051426 1.0 1.1 1.1 1.1 AUTHOR Half Map 1