2015-07-22 OBS 2014-09-02 PDBe PDBe 2014-09-10 2015-07-22
Author's request
Preparing the 60S ribosomal subunit for translation:structural basis of eIF6 release Weis F, Giudice E, Churcher M, Jin L, Hilcenko C, Wong CC, Traynor D, Kay RR, Warren AJ ribosome, ribosomopathy, SBDS, Shwachman-Diamond syndrome, cryo-EM, eIF6, Dictyostelium, EFL1, T-ALL, GTPase, myelodysplastic syndrome, MDS 4uyv Weis F, Giudice E, Churcher M, Jin L, Hilcenko C, Wong CC, Traynor D, Kay RR, Warren AJ Preparing the 60S ribosomal subunit for translation: structural basis of eIF6 release To Be Published emd_2776.map.gz Image stored as Reals 300 300 300 -150 -150 -150 149 149 149 300 300 300 399.0 399.0 399.0 90.0 90.0 90.0 X Y Z -0.33569819 0.64309227 0.00167104 0.03073554 1
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1.33 1.33 1.33 0.08 60S-eIF6-SBDS complex
emd_2776.png
3 Dictyostelium 60S ribosomal subunit bound to endogenous eIF6 in complex with human SBDS 2.9 ribosome-eukaryote 60S ribosomal subunit 2.8
pre60S ribosomal subunit bound to endogenous eIF6
LSU 60S, LSU RNA 28S, LSU RNA 5.8S, LSU RNA 5S Dictyostelium discoideum HM2917 Slime mold false protein eIF6 0.026
Dictyostelium eIF6 bound to endogenous pre60S ribosomal subunit
false HM2917 Dictyostelium discoideum 1 Slime mold IPR002769 Q551M2 protein SBDS 0.029 Homo sapiens 1 Human true Q9Y3A5 IPR002140 pRSETA Escherichia coli BL21(DE3) C41 Blot for 6.5 seconds before plunging ETHANE 100 FEI VITROBOT MARK I Objective lens astigmatism was corrected at 59,000 times magnification FIELD EMISSION GUN 30 BRIGHT FIELD 2000 3000 FLOOD BEAM FEI FALCON II (4k x 4k) 2.7 FEI TITAN KRIOS 03-SEP-2013 FEI TITAN KRIOS AUTOGRID HOLDER 300 59000 3844 particle Quantifoil R2/2 on 300 mesh Cu grid
50 mM HEPES-KOH, 100 mM K(CH3COO), 10 mM Mg(CH3COO)2, 6 mM beta-mercaptoethanol, glutaraldehyde 0.05%
7.5 singleParticle Relion Each particle 3.6 FSC 0.143, gold-standard C1 43063
RELION movement correction processing used