2024-02-07 OBS RCSB RCSB RCSB 2022-06-06 2022-11-23 2022-11-23 2024-02-07 2024-02-07 National Institutes of Health/National Institute of General Medical Sciences (NIH/NIGMS) R35GM137957 United States ELIC5 with cysteamine in 2:1:1 POPC:POPE:POPG nanodisc in open conformation Petroff II JT Deng Z Rau MJ Fitzpatrick JAJ Yuan P Cheng WWL ELIC, ion channel, pLGIC, STRUCTURAL PROTEIN Petroff II JT Dietzen NM Santiago-McRae E Deng B Washington MS Chen LJ Trent Moreland K Deng Z Rau M Fitzpatrick JAJ Yuan P Joseph TT Henin J Brannigan G Cheng WWL Open-channel structure of a pentameric ligand-gated ion channel reveals a mechanism of leaflet-specific phospholipid modulation. Nat Commun UK 13 7017 7017 2022 36385237 doi:10.1038/s41467-022-34813-5 2041-1723 EMD-27219 other EM volume EMD-27218 other EM volume EMD-27217 other EM volume EMD-27216 other EM volume EMD-27215 other EM volume 8d68 FULLOVERLAP ELIC5 (P254G/C300S/V261Y/G319F/I320F) with cysteamine in 2:1:1 POPC:POPE:POPG nanodisc ELIC5 (P254G/C300S/V261Y/G319F/I320F) with cysteamine in 2:1:1 POPC:POPE:POPG nanodisc 0 1 Dickeya dadantii Erwinia ligand-gated ion channel Dickeya dadantii 0.037011055 5 Escherichia coli LEVO APADNAADARPVDVSVSIFINKIYGVNTLEQTYKVDGYIVAQWTGKPRKTPGDKPLIVENTQIERWINNGLWVPALEFIN VVGSPDTGNKRLMLFPDGRVIYNARFLGSFSNDMDFRLFPFDRQQFVLELEPFSYNNQQLRFSDIQVYTENIDNEEIDEW WIRGKASTHISDIRYDHLSSVQPNQNEFSRITVRIDAVRNPSYYLWSFILPLGLIIAASWSVFWLESFSERLQTSFTLML TVVAYAFYTSNILGRLPYTTYIDQMIIAGYGSIFAAILLIIFAHHRQANGVEDDLLIQRSRLAFPLGFLAIGCVLVIRFF TL E0SJQ4 (1R)-2-{[(S)-{[(2S)-2,3-dihydroxypropyl]oxy}(hydroxy)phosphoryl]oxy}-1-[(hexadecanoyloxy)methyl]ethyl (9Z)-octadec-9-enoate 0.0007490069999999999 5 PGW 2-AMINO-ETHANETHIOL 7.7149e-05 5 DHL singleParticle particle 7.5 Quantifoil R2/2 COPPER 300 PLASMA CLEANING OTHER 9.332
H2 27.5 sccm O2 6.4 sccm
 ETHANE 100 277 FEI VITROBOT MARK IV
Blot for 2 seconds before plunging.
FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 150.0 0.01 1.0 1.0 2.5 2.5 105000.0 105000.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN 82.0 84.0 Microscope was equipped with a Cs corrector GIF Bioquantum 20 GATAN K2 SUMMIT (4k x 4k) COUNTING 3838 3710 1 8.0 1.65 1 3.36 FSC 0.143 CUT-OFF 195134 ANGULAR RECONSTITUTION ANGULAR RECONSTITUTION emd_27220.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 180 180 180 0 0 0 180 180 180 208.79999 208.79999 208.79999 90.0 90.0 90.0 X Y Z -0.39578897 0.65561306 0.0015338252 0.025711367 1.16 1.16 1.16 0.0855 AUTHOR 2YN6 PDB experimental model AB INITIO MODEL REAL emd_27220_additional_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 180 180 180 0 0 0 180 180 180 208.79999 208.79999 208.79999 90.0 90.0 90.0 X Y Z -0.03410931 0.13339941 0.0006792439 0.008264159 1.16 1.16 1.16 AUTHOR emd_27220_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 180 180 180 0 0 0 180 180 180 208.79999 208.79999 208.79999 90.0 90.0 90.0 X Y Z -0.04840773 0.14261544 0.000676595 0.008724114 1.16 1.16 1.16 AUTHOR emd_27220_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 180 180 180 0 0 0 180 180 180 208.79999 208.79999 208.79999 90.0 90.0 90.0 X Y Z -0.040096752 0.14558512 0.0006820838 0.008721599 1.16 1.16 1.16 AUTHOR