2015-04-01 OBS 2014-03-10 PDBe PDBe 2014-04-16 2015-04-01 2015-04-01
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Single-particle analysis of KATP channel complex expressed in cultured human cells Kimura Y, Kanno R, Matsuo M, Kato H, Mitsuoka K, Kazumitsu U KATP channel, purification, single-particle analysis Kimura Y, Kanno R, Matsuo M, Kato H, Mitsuoka K, Kazumitsu U Single-particle analysis of KATP channel complex expressed in cultured human cells To Be Published emd_2606.map.gz Image stored as Reals 100 100 100 -50 -49 -50 49 50 49 100 100 100 360.0 360.0 360.0 90.0 90.0 90.0 X Y Z -0.31650114 0.79723728 0.01637996 0.08898084 1
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3.6 3.6 3.6 0.23 Heterooctamer complex of hamster SUR1 and mouse Kir6.2 2 ATP-sensitive potassium channel complex consisting of hamster SUR1 and mouse Kir6.2 Four SUR1 and four Kir6.2 forms heterooctamer 0.88 protein ATP-binding cassette sub-family C member 8 ABCC8, SUR1 0.18 4 Cricetus cricetus Black-bellied hamster true Q09427 Homo sapiens FreeStyle 293F human pCDNA3.1 protein ATP-sensitive inward rectifier potassium channel 11 KCNJ11, Kir6.2 0.044 true 4 Mus musculus Mouse Q61743 pCDNA3.1 FreeStyle 293F Homo sapiens human NONE NONE TUNGSTEN HAIRPIN BRIGHT FIELD SPOT SCAN OTHER 0 38400 0 JEOL 1010 09-JUN-2009 JEOL 100 30000 34 8 Purified protein was stained with 2% uranyl acetate for 10 seconds on a carbon-coated copper grid particle 0.1 400 mesh copper grid with thin carbon support, glow discharged
50 mM HEPES-Na, 50 mM NaCl, 50 mM KCl, 5% glycerol, 1 mM 2-mercaptoethanol, 0.05% C12E8
7.2 singleParticle Cross-common lines IMAGIC 18 FSC 0.5, semi-independent 82 C4 12117
The particles were selected using an automatic selection program.