REL OBS PDBe PDBe PDBe 2014-03-10 2014-04-16 2015-04-01 2015-04-01 2015-04-01 Single-particle analysis of KATP channel complex expressed in cultured human cells Kimura Y Kanno R Matsuo M Kato H Mitsuoka K Kazumitsu U KATP channel, purification, single-particle analysis Kimura Y Kanno R Matsuo M Kato H Mitsuoka K Kazumitsu U Single-particle analysis of KATP channel complex expressed in cultured human cells To Be Published ATP-sensitive potassium channel complex consisting of hamster SUR1 and mouse Kir6.2 ATP-sensitive potassium channel complex consisting of hamster SUR1 and mouse Kir6.2 Four SUR1 and four Kir6.2 forms heterooctamer 2 0.88 ATP-binding cassette sub-family C member 8 Cricetus cricetus Black-bellied hamster 0.18 4 true Homo sapiens human FreeStyle 293F pCDNA3.1 Q09427 ATP-sensitive inward rectifier potassium channel 11 Mus musculus Mouse 0.044 4 true Homo sapiens human FreeStyle 293F pCDNA3.1 Q61743 singleParticle particle 0.1 7.2
50 mM HEPES-Na, 50 mM NaCl, 50 mM KCl, 5% glycerol, 1 mM 2-mercaptoethanol, 0.05% C12E8
 NEGATIVE
Purified protein was stained with 2% uranyl acetate for 10 seconds on a carbon-coated copper grid
400 mesh copper grid with thin carbon support, glow discharged
 NONE OTHER JEOL 1010 SPOT SCAN BRIGHT FIELD TUNGSTEN HAIRPIN 100 30000.0 38400.0 JEOL 2009-06-09 OTHER 34 8.0 0 0
The particles were selected using an automatic selection program.
OTHER 18.0 OTHER IMAGIC 12117 82 emd_2606.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 100 100 100 -50 -49 -50 100 100 100 360.0 360.0 360.0 90.0 90.0 90.0 X Y Z -0.31650114 0.79723728 0.01637996 0.08898084 3.6 3.6 3.6 0.23 AUTHOR Heterooctamer complex of hamster SUR1 and mouse Kir6.2
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