HPUB OBS PDBe PDBe PDBe 2013-09-05 2013-10-09 2015-01-14 2015-01-14 Electron microscopy of the complex formed by chaperones TBCE and TBCB and alpha-tubulin Serna M Carranza G Martin-Benito J Janowsk R Canals A Coll M Zabala JC Valpuesta JM chaperones, protein folding, protein degradation, tubulin proteostasis Serna M Carranza G Martin-Benito J Janowski R Canals A Coll M Zabala JC Valpuesta JM The structure of the complex formed by chaperones TBCE and TBCB and alpha-tubulin reveals a mechanically driven dissociation of the tubulin dimer To Be Published Human Tubulin Binding Cofactor E Human Tubulin Binding Cofactor E Monomer 1 0.059 Tubulin binding cofactor E Homo sapiens Human Cytoplasm 0.059 1 Monomer true Spodoptera frugiperda Sf9 pFastBac-1 Q15813 singleParticle particle 0.01 6.7
100mM MES-NaOH, 25mM KCl, 1mM MgCl2, 1mM EGTA
 NEGATIVE
Grids with adsorbed protein floated on 2% w/v uranyl acetate for 1 min.
300 mesh copper grid with thin carbon support, glow discharged in air atmosphere
 NONE OTHER JEOL 1200EXII FLOOD BEAM BRIGHT FIELD TUNGSTEN HAIRPIN 100 5.6 1.0 1.5 60000.0 JEOL Objective lens astigmatism was corrected at 100,000 times magnification 2011-02-01 KODAK SO-163 FILM ZEISS SCAI 14 68 10
The particles were manually selected using the XMIPP program
N. Grigorieff CTFFIND3
 OTHER 17.0 FSC 0.33 CUT-OFF EMAN, Spider, XMIPP
Individual particles were manually selected using XMIPP software package.
12000 emd_2449.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 24 24 24 0 0 0 24 24 24 111.84 111.84 111.84 90.0 90.0 90.0 X Y Z -0.06966436 0.16886918 0.00422765 0.0253699 4.66 4.66 4.66 0.051 AUTHOR Reconstruction of the human and wildtype TBCE
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