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<emdEntry accessCode="2425" version="1.9.6">
    <admin>
        <lastUpdate>2014-08-27</lastUpdate>
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    <deposition>
        <status prior="HPUB">OBS</status>
        <depositionDate>2013-07-23</depositionDate>
        <depositionSite>PDBe</depositionSite>
        <processingSite>PDBe</processingSite>
        <headerReleaseDate>2013-08-28</headerReleaseDate>
        <obsoletedDate>2014-08-27</obsoletedDate>
        <details>Entry passed its hold period and depositor decided to withdraw the entry.</details>
        <title>Electron microscopy of the PCNA/Polymerase/Fen1/Ligase complex on DNA</title>
        <authors>Cannone G, Beattie T, Bell SD, Spagnolo L</authors>
        <keywords>DNA replication, Sulfolobus solfataricus</keywords>
        <primaryReference published="false">
            <journalArticle>
                <authors>Cannone G, Beattie T, Bell SD, Spagnolo L</authors>
                <articleTitle>The 'Okazakisome'</articleTitle>
                <journal>To Be Published</journal>
                <volume/>
                <firstPage/>
                <lastPage/>
                <year/>
            </journalArticle>
        </primaryReference>
    </deposition>
    <map>
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        <dataType>Image stored as Reals</dataType>
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            <numColumns>64</numColumns>
            <numRows>64</numRows>
            <numSections>64</numSections>
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            <originCol>-5</originCol>
            <originRow>-5</originRow>
            <originSec>-5</originSec>
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        <cell>
            <cellA units="A">409.6</cellA>
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            <cellC units="A">409.6</cellC>
            <cellAlpha units="degrees">90.0</cellAlpha>
            <cellBeta units="degrees">90.0</cellBeta>
            <cellGamma units="degrees">90.0</cellGamma>
        </cell>
        <axisOrder>
            <axisOrderFast>X</axisOrderFast>
            <axisOrderMedium>Y</axisOrderMedium>
            <axisOrderSlow>Z</axisOrderSlow>
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        <statistics>
            <minimum>-18.33686066</minimum>
            <maximum>32.29639816</maximum>
            <average>0.00610720</average>
            <std>1.73493207</std>
        </statistics>
        <spaceGroupNumber>1</spaceGroupNumber>
        <details>::::EMDATABANK.org::::EMD-2425::::</details>
        <pixelSpacing>
            <pixelX units="A">6.4</pixelX>
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            <pixelZ units="A">6.4</pixelZ>
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        <contourLevel source="emdb">4</contourLevel>
        <annotationDetails>Reconstruction of assembled Okazakisome</annotationDetails>
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    <supplement>
        <maskSet/>
        <sliceSet/>
        <figureSet/>
        <fscSet/>
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    <sample>
        <numComponents>4</numComponents>
        <name>Okazakisome</name>
        <compDegree>One Okazakisome bound to DNA</compDegree>
        <sampleComponentList>
            <sampleComponent componentID="1">
                <entry>protein</entry>
                <sciName>PCNA</sciName>
                <protein>
                    <sciSpeciesName ncbiTaxId="2287">Sulfolobus solfataricus</sciSpeciesName>
                    <recombinantExpFlag>true</recombinantExpFlag>
                    <externalReferences/>
                    <natSource/>
                    <engSource/>
                </protein>
            </sampleComponent>
            <sampleComponent componentID="2">
                <entry>nucleic-acid</entry>
                <sciName>DNA</sciName>
                <nucleic-acid>
                    <class>DNA</class>
                    <structure>DOUBLE HELIX</structure>
                    <sciSpeciesName ncbiTaxId="2287">Sulfolobus solfataricus</sciSpeciesName>
                    <syntheticFlag>false</syntheticFlag>
                </nucleic-acid>
            </sampleComponent>
        </sampleComponentList>
    </sample>
    <experiment>
        <vitrification>
            <cryogenName>NONE</cryogenName>
            <instrument>NONE</instrument>
        </vitrification>
        <imaging>
            <electronSource>FIELD EMISSION GUN</electronSource>
            <imagingMode>BRIGHT FIELD</imagingMode>
            <illuminationMode>SPOT SCAN</illuminationMode>
            <microscope>FEI TECNAI F20</microscope>
            <date>04-MAR-2011</date>
            <specimenHolderModel>OTHER</specimenHolderModel>
            <acceleratingVoltage units="kV">200</acceleratingVoltage>
        </imaging>
        <imageAcquisition/>
        <fitting>
            <refProtocol>rigid body</refProtocol>
            <refSpace>REAL</refSpace>
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        <method>singleParticle</method>
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            <numProjections>8206</numProjections>
        </singleParticle>
    </processing>
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