<?xml version="1.0" encoding="UTF-8"?>
<emd emdb_id="EMD-2425" version="3.0.1.9">
    <admin>
        <status_history_list>
            <status status_id="1">
                <code>HPUB</code>
            </status>
        </status_history_list>
        <current_status>
            <code>OBS</code>
            <processing_site>PDBe</processing_site>
        </current_status>
        <sites>
            <deposition>PDBe</deposition>
            <last_processing>PDBe</last_processing>
        </sites>
        <key_dates>
            <deposition>2013-07-23</deposition>
            <header_release>2013-08-28</header_release>
            <obsolete>2014-08-27</obsolete>
            <update>2014-08-27</update>
        </key_dates>
        <title>Electron microscopy of the PCNA/Polymerase/Fen1/Ligase complex on DNA</title>
        <authors_list>
            <author>Cannone G</author>
            <author>Beattie T</author>
            <author>Bell SD</author>
            <author>Spagnolo L</author>
        </authors_list>
        <keywords>DNA replication, Sulfolobus solfataricus</keywords>
    </admin>
    <crossreferences>
        <citation_list>
            <primary_citation>
                <journal_citation published="false">
                    <author order="1">Cannone G</author>
                    <author order="2">Beattie T</author>
                    <author order="3">Bell SD</author>
                    <author order="4">Spagnolo L</author>
                    <title>The 'Okazakisome'</title>
                    <journal>To Be Published</journal>
                </journal_citation>
            </primary_citation>
        </citation_list>
    </crossreferences>
    <sample>
        <name>Okazakisome</name>
        <supramolecule_list>
            <sample_supramolecule supramolecule_id="1000">
                <name>Okazakisome</name>
                <oligomeric_state>One Okazakisome bound to DNA</oligomeric_state>
                <number_unique_components>4</number_unique_components>
            </sample_supramolecule>
        </supramolecule_list>
        <macromolecule_list>
            <protein_or_peptide macromolecule_id="1">
                <name>PCNA</name>
                <natural_source database="NCBI">
                    <organism ncbi="2287">Sulfolobus solfataricus</organism>
                </natural_source>
                <recombinant_exp_flag>true</recombinant_exp_flag>
                <recombinant_expression database="NCBI"/>
                <sequence/>
            </protein_or_peptide>
            <dna macromolecule_id="2">
                <name>DNA</name>
                <natural_source database="NCBI">
                    <organism ncbi="2287">Sulfolobus solfataricus</organism>
                </natural_source>
                <classification>DNA</classification>
                <structure>DOUBLE HELIX</structure>
                <synthetic_flag>false</synthetic_flag>
            </dna>
        </macromolecule_list>
    </sample>
    <structure_determination_list>
        <structure_determination structure_determination_id="1">
            <method>singleParticle</method>
            <aggregation_state>particle</aggregation_state>
            <specimen_preparation_list>
                <single_particle_preparation preparation_id="1">
                    <vitrification>
                        <cryogen_name>NONE</cryogen_name>
                        <instrument>OTHER</instrument>
                    </vitrification>
                </single_particle_preparation>
            </specimen_preparation_list>
            <microscopy_list>
                <single_particle_microscopy microscopy_id="1">
                    <microscope>FEI TECNAI F20</microscope>
                    <illumination_mode>SPOT SCAN</illumination_mode>
                    <imaging_mode>BRIGHT FIELD</imaging_mode>
                    <electron_source>FIELD EMISSION GUN</electron_source>
                    <acceleration_voltage units="kV">200</acceleration_voltage>
                    <specimen_holder_model>OTHER</specimen_holder_model>
                    <date>2011-03-04</date>
                </single_particle_microscopy>
            </microscopy_list>
            <singleparticle_processing image_processing_id="1">
                <final_reconstruction>
                    <number_images_used>8206</number_images_used>
                </final_reconstruction>
            </singleparticle_processing>
        </structure_determination>
    </structure_determination_list>
    <map format="CCP4" size_kbytes="1025">
        <file>emd_2425.map.gz</file>
        <symmetry>
            <space_group>1</space_group>
        </symmetry>
        <data_type>IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)</data_type>
        <dimensions>
            <col>64</col>
            <row>64</row>
            <sec>64</sec>
        </dimensions>
        <origin>
            <col>-5</col>
            <row>-5</row>
            <sec>-5</sec>
        </origin>
        <spacing>
            <x>64</x>
            <y>64</y>
            <z>64</z>
        </spacing>
        <cell>
            <a units="Å">409.6</a>
            <b units="Å">409.6</b>
            <c units="Å">409.6</c>
            <alpha units="deg">90.0</alpha>
            <beta units="deg">90.0</beta>
            <gamma units="deg">90.0</gamma>
        </cell>
        <axis_order>
            <fast>X</fast>
            <medium>Y</medium>
            <slow>Z</slow>
        </axis_order>
        <statistics>
            <minimum>-18.33686066</minimum>
            <maximum>32.29639816</maximum>
            <average>0.0061072</average>
            <std>1.73493207</std>
        </statistics>
        <pixel_spacing>
            <x units="Å">6.4</x>
            <y units="Å">6.4</y>
            <z units="Å">6.4</z>
        </pixel_spacing>
        <contour_list>
            <contour primary="true">
                <level>4.0</level>
                <source>EMDB</source>
            </contour>
        </contour_list>
        <annotation_details>Reconstruction of assembled Okazakisome</annotation_details>
        <details>::::EMDATABANK.org::::EMD-2425::::</details>
    </map>
    <interpretation>
        <modelling_list>
            <modelling>
                <initial_model>
                    <access_code>1ul1</access_code>
                </initial_model>
                <refinement_protocol>RIGID BODY FIT</refinement_protocol>
                <refinement_space>REAL</refinement_space>
            </modelling>
        </modelling_list>
    </interpretation>
</emd>
