REL OBS PDBe PDBe PDBe 2013-05-24 2013-07-03 2013-10-02 2013-10-02 2013-10-02 Organization of protein complexes and a mechanism for grana formation in photosynthetic membranes as revealed by cryo-electron microscopy. Ford RC Holzenburg A Photosynthesis, thylakoid membrane, CF1-CF0 proton ATPase, photosystem, cytochrome b6f, ribosome, chloroplast. Ford RC Holzenburg A Organization of protein complexes and a mechanism for grana formation in photosynthetic membranes as revealed by cryo-electron microscopy Crystal Research and Technology 2013 doi:10.1002/crat.201300116 Thylakoid membrane from spinach. Thylakoid membrane from spinach. 1 Thylakoid membrane
Buffer - 20mM MES, 5mM MgCl2, 15mM NaCl, pH6.5, 150mM sorbitol. 3microlitres of membranes at 2mg per ml chlorophyll were directly applied to electron microscope grids (Quantifoil carbon grids with 1.2 micron holes). After blotting excess liquid, (2x 1s blots at 95 percent relative humidity in a Vitrobot device), the grids were rapidly frozen by plunging into liquid ethane and transferred at less than 110K to the Gatan cryo-stage of the electron microscope. 10nm fiducial gold particles were added.
false Spinacia oleracea Spinach Leaf Chloroplast tomography tissue 2 6.5
20mM MES, 5mM MgCl2, 15mM NaCl, pH6.5, 150mM sorbitol.
 NEGATIVE
No stain.
Quantifoil 400mesh carbon grids with 1.2 micron holes.
 NITROGEN 95 97 FEI VITROBOT MARK I
Vitrification instrument: Vitrobot FEI. Rapid plunging into liquid ethane.
2 x 1 sec blots before plunging. FEI TECNAI F20 FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 200 2.0 8.0 8.0 34000.0 33998.0 GATAN LIQUID NITROGEN 96 98 97 Astigmatism corrected by live FFT. 2009-07-28 15 57 45 Single tilt -65 49 1
1 degree increments above 45 deg, 2.5 degree increments below this.
45.0 OTHER eTOMO
Standard eTOMO procedures for single axis tilt tomograms. Fiducials were selected from the tomogram and tracked in each image by hand initially.
57 emd_2384.map.gz 1 IMAGE STORED AS SIGNED INTEGER (2 BYTES) 690 578 125 -57 -87 6 578 690 125 6072.0 5086.4 1100.0 90.0 90.0 90.0 X Y Z 23.0 236.0 86.96832275 5.27337313 8.8 8.8 8.8 94.0 AUTHOR Tomogram of stacked thylakoid membranes. Includes two dimensional crystal of photosystem II complexes in a grana membrane.
::::EMDATABANK.org::::EMD-2384::::
emd_2384.tif