2014-05-21 OBS 2013-05-08 PDBe PDBe 2013-06-05 2014-05-21
updated map in EMD-5954
Electron cryo-microscopy of marine virus Syn. Gipson P, Baker ML, Liu X, Raytcheva D, Haase C, Piret J, King J, Chiu W Syn5, marine virus, Synechococcus, cyanophage, cyanobacteria, outer capsid protein, maturation 4bml Gipson P, Baker ML, Liu X, Raytcheva D, Haase C, Piret J, King J, Chiu W A distinct structural arrangement obtained by an ancient marine virus during maturation To Be Published emd_2371.map.gz Image stored as Reals 544 544 544 -272 -272 -272 271 271 271 544 544 544 718.08 718.08 718.08 90.0 90.0 90.0 X Y Z -0.70279670 1.06672800 0.01083561 0.06751607 1
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1.32 1.32 1.32 0.26 Reconstruction of icosahedral symmetric map of marine virus Syn5
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1 Mature capsid of marine virus Syn5 virus Synechococcus phage Syn5 horned virus Synechococcus phage Syn5 horned virus false false SPECIES VIRION WH 8109 Synechococcus sp. WH 8109 BACTERIA(EUBACTERIA) 640 7 Blot for 1 second before plunging. ETHANE 90 FEI VITROBOT MARK IV FIELD EMISSION GUN 23 Omega BRIGHT FIELD 0.7 3.5 FLOOD BEAM GATAN ULTRASCAN 10000 (10k x 10k) 4.1 JEOL 3200FSC 28-AUG-2010 JEOL 3200FSC CRYOHOLDER 300 80000 0-25 1000 particle 400 mesh Cu grid R1.2/1.3 grids singleParticle Cross-common lines Multi-path, Simulated, Annealing, (MPSA) each frame 5.7 1) FSC at 0.5 cut-off between conventional even and odd maps 2) FSC between subunits of an asymmetric unit at FSC=0.33 I 9700
The particles were selected using EMAN2, while 3D reconstruction was performed by multi-path simulated annealing (MPSA) program.