2024-10-09 OBS RCSB RCSB RCSB 2020-11-09 2021-05-05 2021-05-05 2024-10-09 2024-10-09 Nucleocapsid protein from the SARS-CoV-2 coronavirus Casasanta MA Kelly DF Nucleocapsid, SARS-CoV-2, Coronavirus, N-protein, VIRAL PROTEIN Casasanta MA Jonaid GM Kaylor L Luqiu WY Solares MJ Schroen ML Dearnaley WJ Wilson J Dukes MJ Kelly DF Retraction: Microchip-based structure determination of low-molecular weight proteins using cryo-electron microscopy. Nanoscale UK 16 15834 15834 2024 39129655 doi:10.1039/d4nr90151g 2040-3372 Casasanta MA Jonaid GM Kaylor L Luqiu WY Solares MJ Schroen ML Dearnaley WJ Wilson J Dukes MJ Kelly DF Microchip-based structure determination of low-molecular weight proteins using cryo-electron microscopy. Nanoscale UK 13 7285 7293 2021 33889923 doi:10.1039/d1nr00388g 2040-3372 SARS-CoV-2 Severe acute respiratory syndrome coronavirus 2 0 Severe acute respiratory syndrome coronavirus 2 Homo sapiens 0.05 VIRION STRAIN true true singleParticle particle 0.1 7.5 20.0 Tris 150.0 NaCl sodium chloride 10.0 MgCl2 magnesium chloride 10.0 CaCl2 calcium chloride
Solutions were made fresh before diluting protein and adding to the EM grids
 Homemade SILICON NITRIDE CONTINUOUS 20.0 ETHANE 95 293 FEI VITROBOT MARK III
blot for 7.5 seconds before plunging.
Sample was monodisperse on a silicon nitride microchip coated with 25% Ni-NTA lipids.
 TFS TALOS F200C FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 200 2.7 0.5 1.5 45000.0 GATAN 626 SINGLE TILT LIQUID NITROGEN CRYO TRANSFER HOLDER NITROGEN 75.0 83.0 DIRECT ELECTRON DE-12 (4k x 3k) INTEGRATING 3 0.25 5.0
Images were collected in movie mode with 0.25 second exposures at 30 frames per second.
 1 20000 C1 4.3 FSC 0.143 CUT-OFF cryoSPARC 20000 MAXIMUM LIKELIHOOD cryoSPARC MAXIMUM LIKELIHOOD cryoSPARC 1 20000.0 cryoSPARC emd_22982.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 150 150 150 150 150 150 150 150 150 139.5 139.5 139.5 90.0 90.0 90.0 X Y Z -0.0 0.0258326 0.00013124387 0.0010522302 0.93 0.93 0.93 0.0035 AUTHOR Nucleocapsid protein from the SARS-CoV-2 Coronavirus FLEXIBLE FIT REAL