2021-02-01 OBS RCSB RCSB RCSB 2020-01-29 2021-02-10 2021-02-10 2021-02-01 Suppressed Suppressed Suppressed Suppressed Suppressed X Suppressed X Suppressed X Suppressed X Suppressed X Suppressed X Suppressed X Suppressed X Suppressed X Suppressed X Suppressed Suppressed EMD-21255 associated EM volume
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 Suppressed Suppressed 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84
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Suppressed Suppressed Kidney HEK293 Endoplasmic Reticulum Endoplasmic Reticulum 1 Suppressed 1 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44
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Suppressed Suppressed Kidney HEK293 Endoplasmic Reticulum Endoplasmic Reticulum 1 Suppressed 1 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76
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Suppressed Suppressed Kidney HEK293 Endoplasmic Reticulum Endoplasmic Reticulum 1 Suppressed 1 77 78 79 80 81 82 83 84
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Suppressed Suppressed Kidney HEK293 Endoplasmic Reticulum Endoplasmic Reticulum 1 Suppressed 4 82 83 84
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Suppressed Suppressed Kidney HEK293 Endoplasmic Reticulum Endoplasmic Reticulum 1 Suppressed 4 77
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Suppressed Suppressed Kidney HEK293 Endoplasmic Reticulum Endoplasmic Reticulum 1 Suppressed 4 79 80 81
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Suppressed Suppressed Kidney HEK293 Endoplasmic Reticulum Endoplasmic Reticulum 1 Suppressed 4 78
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Suppressed Suppressed Kidney HEK293 Endoplasmic Reticulum Endoplasmic Reticulum 1 Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X Suppressed Suppressed kidney 1e-06 LEVO X singleParticle particle 0.4 7.4 150.0 C2H3KO2 potassium acetate 50.0 C8H18N2O4S HEPES, pH 7.4 5.0 MgCl2 magnesium chloride 0.25 C56H92O29 digitonin C-flat-1.2/1.3 COPPER 200 CARBON CONTINUOUS 2.0 GLOW DISCHARGE ETHANE 100 298 FEI VITROBOT MARK IV
Two filter papers were added to each arm, 2.5 microliters of sample were added to grids, samples were blotted for 11 seconds, and 0.5 seconds of drain time was allowed before vitrification..
Sample was well-dispersed on a thin (~2 nm) carbon film.
 FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 GATAN K3 (6k x 4k) COUNTING 1-40 1 5562 3.8 50.0 1 1049128 Gctf 0.5
EMD-5592, low pass-filtered to 50 Angstrom resolution
 C1 4.2 FSC 0.143 CUT-OFF RELION 3.0 151980 MAXIMUM LIKELIHOOD RELION 3.0 MAXIMUM LIKELIHOOD RELION 3.0 12 RELION 3.0 emd_21255.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 500 500 500 0 0 0 500 500 500 680.0 680.0 680.0 90.0 90.0 90.0 X Y Z -0.01577375 0.046370506 -7.704659e-05 0.0017383097 1.36 1.36 1.36 0.005 AUTHOR Suppressed emd_21255_additional_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 500 500 500 0 0 0 500 500 500 680.0 680.0 680.0 90.0 90.0 90.0 X Y Z 0.0 1.0 0.0053420393 0.07111543 1.36 1.36 1.36 AUTHOR Suppressed emd_21255_half_map_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 500 500 500 0 0 0 500 500 500 680.0 680.0 680.0 90.0 90.0 90.0 X Y Z -0.02045565 0.051616006 -1.9284358e-05 0.0018870222 1.36 1.36 1.36 AUTHOR Suppressed emd_21255_half_map_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 500 500 500 0 0 0 500 500 500 680.0 680.0 680.0 90.0 90.0 90.0 X Y Z -0.022475647 0.054168962 -2.4848341e-05 0.0018872631 1.36 1.36 1.36 AUTHOR Suppressed