2021-02-01 OBS RCSB RCSB RCSB 2020-01-12 2021-02-10 2021-02-10 2021-02-01 National Health and Medical Research Council (NHMRC, Australia) APP1159347 Australia National Health and Medical Research Council (NHMRC, Australia) APP1146403 Australia Cryo-EM structure of octadecameric TF55 (beta-only) complex from S. solfataricus (C1 non-symmetric) Zeng YC Sobti M Stewart AG Zeng YC Sobti M Stewart AG Structural Analysis of a Filamentous Chaperonin from Sulfolobus solfataricus To Be Published 0353 EMD-21208 other EM volume
D9 symmetric map
 EMD-21210 associated EM volume Octadecameric complex of TF55 chaperonin beta subunit Octadecameric complex of TF55 chaperonin beta subunit 0 1 Saccharolobus solfataricus 1.08 singleParticle particle 20 8.0 20.0 C4H11NO3 tris(hydroxymethyl)aminomethane hydrochloride 2.0 MgCl2 magnesium chloride 1.0 C10H16N2O8 Ethylenediaminetetraacetic acid 100.0 NaCl sodium chloride ETHANE 100 295 FEI VITROBOT MARK IV
blot 5 seconds.
FEI TALOS ARCTICA FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 200 NITROGEN FEI FALCON III (4k x 4k) COUNTING 50.0 1 CTFFIND 4.1.13 EMD-6291
Low-pass filtered to 30 angstroms as reference model for 3D classification after initial auto-picking of micrograph subset to afford new 3D template (low-pass filtered to 20 angstroms) for auto-picking across full data set. The 3D template for auto-picking (low-pass filtered to 20 angstroms) was used as the reference for 3D classification of all picked particles.
 C1 4.38 FSC 0.143 CUT-OFF RELION 3.0 43117 MAXIMUM LIKELIHOOD RELION 3.0 PROJECTION MATCHING RELION 3.0 emd_21210.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 300 300 300 0 0 0 300 300 300 294.0 294.0 294.0 90.0 90.0 90.0 X Y Z -0.06565606 0.12288419 0.000910851 0.0054642647 0.98 0.98 0.98 0.02 AUTHOR C1 non-symmetric