2013-04-17 OBS 2012-02-07 PDBe PDBe 2012-02-15 2013-02-27 2013-04-17
Author withdrawal of the entry.
Yersinia enterocolitica Injectisome in situ Kudryashev M, Amstutz M, Castano-Diez D, Bleck CKE, Kowal J, Cornelis GR, Stahlberg H Type III secretion, needle complex Kudryashev M, Amstutz M, Castano-Diez D, Bleck CKE, Kowal J, Cornelis GR, Stahlberg H In situ structure of the Yersinia enterocolitica injectisome To Be Published emd_2042.map.gz Image stored as Reals 128 128 128 0 0 0 127 127 127 128 128 128 94.72 94.72 94.72 90.0 90.0 90.0 X Y Z -4.04227829 5.45056105 -0.00742681 1.00132513 1
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0.74 0.74 0.74 Structure of Yersinia enterocolitica injectisome in situ, wild type 1 Entire Injectisome/type III secretion system of Yersinia enterocolitica
Individual injectisomes were extracted from tomograms of intact bacterial cells
cellular-component Entire Injectisome/type III secretion system of Yersinia enterocolitica false Yersinia enterocolitica Blot for 2 seconds before plunging ETHANE 90 FEI VITROBOT MARK IV FIELD EMISSION GUN 20 Gatan post column Image Filter BRIGHT FIELD -2000 -15000 FLOOD BEAM GATAN ULTRASCAN 1000 (2k x 2k) 2.8 -60 60 100 FEI TITAN KRIOS 01-JAN-2010 OTHER 300 19500 0-20 non-stained Quantifoil holey carbon grid with 3.5/1 spacing
PBS
 subtomogramAveraging 6d-search with missing wedge compensation AV3,Dynamo Focal pair tomography 37 1-bit information threshold
We used focal pair tomography in a combination with the standard sub-tomogram processing routines in AV3 (Forster et al, PNAS 2005) and Dynamo (Castano-Diez et al, 2012, www.dynamo-em.org). Average number of tilts used in the 3D reconstructions: 61. Average tomographic tilt angle increment: 2.