HOLD OBS PDBe PDBe PDBe 2012-02-07 2012-02-15 2013-02-27 2013-04-17 2013-04-17 Yersinia enterocolitica Injectisome in situ Kudryashev M Amstutz M Castano-Diez D Bleck CKE Kowal J Cornelis GR Stahlberg H Type III secretion, needle complex Kudryashev M Amstutz M Castano-Diez D Bleck CKE Kowal J Cornelis GR Stahlberg H In situ structure of the Yersinia enterocolitica injectisome To Be Published Entire Injectisome/type III secretion system of Yersinia enterocolitica Entire Injectisome/type III secretion system of Yersinia enterocolitica
Individual injectisomes were extracted from tomograms of intact bacterial cells
1 Entire Injectisome/type III secretion system of Yersinia enterocolitica false Yersinia enterocolitica subtomogramAveraging
PBS
 NEGATIVE
non-stained
Quantifoil holey carbon grid with 3.5/1 spacing
 ETHANE 90 FEI VITROBOT MARK IV Blot for 2 seconds before plunging FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 2.8 -2.0 -15.0 19500.0 OTHER 100 Gatan post column Image Filter 0.0 20.0 2010-01-01 GATAN ULTRASCAN 1000 (2k x 2k) 20 -60 60
We used focal pair tomography in a combination with the standard sub-tomogram processing routines in AV3 (Forster et al, PNAS 2005) and Dynamo (Castano-Diez et al, 2012, www.dynamo-em.org). Average number of tilts used in the 3D reconstructions: 61. Average tomographic tilt angle increment: 2.
OTHER 37.0 OTHER AV3,Dynamo
Focal pair tomography
 emd_2042.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 128 128 128 0 0 0 128 128 128 94.72 94.72 94.72 90.0 90.0 90.0 X Y Z -4.04227829 5.45056105 -0.00742681 1.00132513 0.74 0.74 0.74 Structure of Yersinia enterocolitica injectisome in situ, wild type
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