2021-02-01 OBS 2019-06-17 RCSB RCSB 2021-02-10 2021-02-10 Suppressed Suppressed X, Suppressed X, Suppressed X, Suppressed X, Suppressed X, Suppressed X 6pcj Suppressed X, Suppressed X, Suppressed X, Suppressed X, Suppressed X, Suppressed X Suppressed Suppressed emd_20302.map.gz Image stored as Reals 400 400 400 0 0 0 399 399 399 400 400 400 303.59998 303.59998 303.59998 90.0 90.0 90.0 X Y Z -1.1045548 3.1610682 0.008142249 0.119651094 1
0.75899994 0.75899994 0.75899994 0.417 Suppressed 2 Suppressed
Suppressed
protein Suppressed 1
Suppressed
Suppressed Suppressed false Suppressed protein Suppressed 1e-06 Suppressed 12 false Suppressed ETHANE 85 298 LEICA EM GP
Grids were first glow-discharged at 15 mA for 1 min using PELCO easiGlow. Then 3 uL of protein sample was pipetted on the carbon side and blotted for 3 seconds..
FIELD EMISSION GUN 1.5 BRIGHT FIELD FLOOD BEAM GATAN K2 SUMMIT (4k x 4k) 2.7 FEI TITAN KRIOS OTHER 300 particle 0.2 7.5
50 mM Tris, pH 7.5, 150 mM NaCl, 4 mM DTT
 at 15 mA, Pelco EASYGLOW (Ted Pella) singleParticle cryoSPARC 3.16 FSC 0.143 CUT-OFF D6 265224