2021-02-01 OBS 2019-04-25 RCSB RCSB 2021-02-10 2021-02-10 Reconstruction of class 2 of CD4- and 17-bound B41 HIV-1 Env SOSIP incubated with LMNG and small molecule GO27 Ozorowski G, Torres JL, Ward AB Ozorowski G, Torres JL, Santos-Martins D, Forli S, Ward AB CryoEM structure guided design of a new class of small molecules that target a pocket near the fusion peptide of receptor bound HIV-1 Env To Be Published 0353 emd_20154.map.gz Image stored as Reals 288 288 288 0 0 0 287 287 287 288 288 288 296.63998 296.63998 296.63998 90.0 90.0 90.0 X Y Z -1.0728008 1.2932017 -7.328816e-05 0.055898637 1
1.03 1.03 1.03 0.3 sharpened map 6 Class 2 of CD4- and 17b-bound HIV-1 Env B41 SOSIP incubated with LMNG and small molecule GO27 protein Class 2 of CD4- and 17b-bound HIV-1 Env B41 SOSIP incubated with LMNG and small molecule GO27 0.05 Human immunodeficiency virus 1 false protein HIV-1 B41 SOSIP gp120 Human immunodeficiency virus 1 false Homo sapiens protein HIV-1 B41 SOSIP gp41 Human immunodeficiency virus 1 false Homo sapiens protein T-cell surface glycoprotein CD4 Homo sapiens false Homo sapiens protein 17b Fab light chain Homo sapiens false Homo sapiens protein 17b Fab heavy chain Homo sapiens false Homo sapiens ETHANE 100 283 FEI VITROBOT MARK IV FIELD EMISSION GUN 51.0 BRIGHT FIELD 500.0 2500.0 FLOOD BEAM GATAN K2 SUMMIT (4k x 4k) 2.7 FEI TITAN KRIOS FEI TITAN KRIOS AUTOGRID HOLDER 300 29000 5296 5.0 5VN3 rigid body particle 5 7.4
Detergent (LMNG) added shortly (<5 minutes) prior to grid freezing using an 8X concentrated stock.
 N/A singleParticle cryoSPARC 4.0 FSC 0.143 CUT-OFF
FSC resolution estimation calculated in CryoSPARC 2 and Relion 3.0
 C3 51358