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    <admin>
        <current_status>
            <date>2021-02-01</date>
            <code>OBS</code>
            <processing_site>RCSB</processing_site>
        </current_status>
        <sites>
            <deposition>RCSB</deposition>
            <last_processing>RCSB</last_processing>
        </sites>
        <key_dates>
            <deposition>2019-04-07</deposition>
            <header_release>2021-02-10</header_release>
            <obsolete>2021-02-10</obsolete>
            <update>2021-02-01</update>
        </key_dates>
        <grant_support>
            <grant_reference>
                <funding_body>Howard Hughes Medical Institute</funding_body>
                <code>55108536</code>
                <country>United States</country>
            </grant_reference>
            <grant_reference>
                <funding_body>National Institutes of Health/National Institute of General Medical Sciences</funding_body>
                <code>R01-GM086451</code>
                <country>United States</country>
            </grant_reference>
            <grant_reference>
                <funding_body>National Institutes of Health/National Institute of General Medical Sciences</funding_body>
                <code>R01-GM117093</code>
                <country>United States</country>
            </grant_reference>
        </grant_support>
        <title>Subunit joining exposes nascent pre-40S rRNA for processing and quality control</title>
        <authors_list>
            <author>Rai J</author>
            <author>Parker MD</author>
            <author>Ghalei H</author>
            <author>Johnson MC</author>
            <author>Karbstein K</author>
            <author>Stroupe ME</author>
        </authors_list>
    </admin>
    <crossreferences>
        <citation_list>
            <primary_citation>
                <journal_citation published="false">
                    <author order="1">Rai J</author>
                    <author order="2">Karbstein K</author>
                    <author order="3">Stroupe ME</author>
                    <author order="4">Ghalei H</author>
                    <author order="5">Johnson MC</author>
                    <author order="6">Parker MD</author>
                    <title>Subunit joining exposes nascent pre-40S rRNA for processing and quality control</title>
                    <journal_abbreviation>To Be Published</journal_abbreviation>
                    <external_references type="CSD">0353</external_references>
                </journal_citation>
            </primary_citation>
        </citation_list>
        <emdb_list>
            <emdb_reference>
                <emdb_id>EMD-20072</emdb_id>
                <relationship>
                    <other>associated EM volume</other>
                </relationship>
            </emdb_reference>
        </emdb_list>
    </crossreferences>
    <sample>
        <name>80S-like ribosome</name>
        <supramolecule_list>
            <complex_supramolecule supramolecule_id="1">
                <name>80S-like ribosome</name>
                <parent>0</parent>
                <macromolecule_list>
                    <macromolecule>
                        <macromolecule_id>1</macromolecule_id>
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                    <macromolecule>
                        <macromolecule_id>2</macromolecule_id>
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                    <macromolecule>
                        <macromolecule_id>3</macromolecule_id>
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                    <macromolecule>
                        <macromolecule_id>4</macromolecule_id>
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                    <macromolecule>
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                    <macromolecule>
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                    <macromolecule>
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                    <macromolecule>
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                    <macromolecule>
                        <macromolecule_id>9</macromolecule_id>
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                    <macromolecule>
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                    <macromolecule>
                        <macromolecule_id>11</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>12</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>13</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>14</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>15</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>16</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>17</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>18</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>19</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>20</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>21</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>22</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>23</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>24</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>25</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>26</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>27</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>28</macromolecule_id>
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                    <macromolecule>
                        <macromolecule_id>29</macromolecule_id>
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                    <macromolecule>
                        <macromolecule_id>30</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>31</macromolecule_id>
                    </macromolecule>
                    <macromolecule>
                        <macromolecule_id>32</macromolecule_id>
                    </macromolecule>
                </macromolecule_list>
                <natural_source database="NCBI">
                    <organism ncbi="4932">Saccharomyces cerevisiae</organism>
                </natural_source>
            </complex_supramolecule>
        </supramolecule_list>
    </sample>
    <structure_determination_list>
        <structure_determination structure_determination_id="1">
            <method>singleParticle</method>
            <aggregation_state>particle</aggregation_state>
            <specimen_preparation_list>
                <single_particle_preparation preparation_id="1">
                    <buffer>
                        <ph>6.8</ph>
                    </buffer>
                    <vitrification>
                        <cryogen_name>ETHANE</cryogen_name>
                    </vitrification>
                </single_particle_preparation>
            </specimen_preparation_list>
            <microscopy_list>
                <single_particle_microscopy microscopy_id="1">
                    <microscope>FEI TITAN KRIOS</microscope>
                    <illumination_mode>FLOOD BEAM</illumination_mode>
                    <imaging_mode>BRIGHT FIELD</imaging_mode>
                    <electron_source>FIELD EMISSION GUN</electron_source>
                    <acceleration_voltage units="kV">300</acceleration_voltage>
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                        <image_recording image_recording_id="1">
                            <film_or_detector_model>DIRECT ELECTRON DE-64 (8k x 8k)</film_or_detector_model>
                            <detector_mode>COUNTING</detector_mode>
                            <average_electron_dose_per_image units="e/Å^2">25.0</average_electron_dose_per_image>
                        </image_recording>
                    </image_recording_list>
                </single_particle_microscopy>
            </microscopy_list>
            <singleparticle_processing image_processing_id="1">
                <image_recording_id>1</image_recording_id>
                <final_reconstruction>
                    <resolution units="Å" res_type="BY AUTHOR">5.2</resolution>
                    <resolution_method>FSC 0.143 CUT-OFF</resolution_method>
                    <number_images_used>17030</number_images_used>
                </final_reconstruction>
                <initial_angle_assignment>
                    <type>NOT APPLICABLE</type>
                </initial_angle_assignment>
                <final_angle_assignment>
                    <type>NOT APPLICABLE</type>
                    <software_list>
                        <software>
                            <name>RELION</name>
                            <version>3</version>
                        </software>
                    </software_list>
                </final_angle_assignment>
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        <file>emd_20072.map.gz</file>
        <symmetry>
            <space_group>1</space_group>
        </symmetry>
        <data_type>IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)</data_type>
        <dimensions>
            <col>384</col>
            <row>384</row>
            <sec>384</sec>
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            <row>0</row>
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        <spacing>
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            <y>384</y>
            <z>384</z>
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        <cell>
            <a units="Å">476.16</a>
            <b units="Å">476.16</b>
            <c units="Å">476.16</c>
            <alpha units="deg">90.0</alpha>
            <beta units="deg">90.0</beta>
            <gamma units="deg">90.0</gamma>
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        <axis_order>
            <fast>X</fast>
            <medium>Y</medium>
            <slow>Z</slow>
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        <statistics>
            <minimum>-0.0957283</minimum>
            <maximum>0.19997369</maximum>
            <average>0.00037524107</average>
            <std>0.0041243853</std>
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        <pixel_spacing>
            <x units="Å">1.24</x>
            <y units="Å">1.24</y>
            <z units="Å">1.24</z>
        </pixel_spacing>
        <contour_list>
            <contour primary="true">
                <level>0.0161</level>
                <source>AUTHOR</source>
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        </contour_list>
        <label>::::EMDATABANK.org::::EMD-20072::::                                             </label>
    </map>
    <interpretation>
        <modelling_list>
            <modelling>
                <refinement_space>REAL</refinement_space>
            </modelling>
        </modelling_list>
    </interpretation>
</emd>
