2023-12-20 OBS PDBe PDBe PDBe 2023-06-16 2023-07-19 2023-07-19 2023-12-20 2023-12-20 Grant Agency of the Czech Republic GX19-25982X Czech Republic European Research Council (ERC) 101043454 European Union Composite map of the Emiliania huxleyi virus 201 (EhV-201) symmetry expanded from cryo-EM structure of the virion vertex with a diameter of 120 nm. Homola M Buttner CR Fuzik T Novacek J Chaillet M Forster F Plevka P cryo-EM, subtomogram averaging, EhV-201, enveloped virus, capsid, major capsid protein, composite map, VIRUS Homola M Buttner CR Fuzik T Novacek J Chaillet M Forster F Plevka P Emiliania huxleyi virus 201 (EhV-201) asymmetrical unit assembled from EhV-201 major capsid protein AlphaFold2 predicted structure and Paramecium bursaria chlorella virus 1 (PBCV-1) penton protein fitted into the EhV-201 virion composite map. To Be Published 0353 EMD-17648 other EM volume
Emiliania huxleyi virus 201 (EhV-201) major capsid protein structure - focused map
 EMD-17823 other EM volume
Consensus map
 EMD-17651 other EM volume
Focused Refinement
 8pfn FULLOVERLAP Emiliania huxleyi virus 201 Emiliania huxleyi virus 201 0 1 2
EhV-201 was propagated on a non-calcifying Emiliania huxleyi strain (CCPM 2090).
Emiliania huxleyi CCMP1516 CCMP 2090 inner membrane capsid 1990.0 169 outer membrane 2110.0 VIRION SPECIES true false Major capsid protein Emiliania huxleyi virus 201 0.053843402
AlphaFold2 predicted structure.
84 LEVO AGSLTQLLATGSMDAALTQNATRTFWKSSYQKHSLFALESINQPFTTQVQFGAESHITVNRQGDLLSWMYLKIVLPGLKV QNQADTVQPTQQSFASLDNDVAAQADVSHVLPYIEGAYTEASLNTKEQLIAEAKNSYEAAKYNAAPLPVAAQMQSTEMPD FDYAYWTEAIGFHLIKRAEFKVGGATIDTIWSELLFAMEELMGRAGRRLTETIGRTLRRPTELMKASRQEQILYVPLPWY FTKHPSLAFPLVAATYHNIQLWVQWAQLNSCIIKSRSNLVVLHAERNVPISDDHLRASLECTYVHLEAAERDALTANAGT QLIVQHQAHLQQVSSNNVTARLNFNFPVLEFYYFLRRKANKDAGDHFNFSGIGGRDPVVSAELLFNNTARVTQKPAVWWR AVQALQFHSSAPLTNIYSYSFSLSPEDPITPSGSANFSRLDSVELALTLQDDFGAAHDANSELFVFARSYNILKFTNGLA GL G9E4T6 Paramecium bursaria chlorella virus 1 (PBCV-1) penton protein. Paramecium bursaria Chlorella virus 1 0.01789217 1 LEVO VETTQHFVSIESSNRPDPANTTPANYSIQLPQRYRNIWSAMLVNIALPAVSPPQKYVYLDIDKLNSIDSTSPSGGVNFAL AKIPLSIAGTGNVFFADTMTSSFPNVPLQNPVATMDKLNIKLKDANGNVLTIPAGNEHSFMIQLTCGDYIPRGGGSTITQ NGRVLGG Q84626 subtomogramAveraging particle 8.0 sea salt Quantifoil R2/1 COPPER 200 CARBON HOLEY 12.0 GLOW DISCHARGE OTHER 5e-05
top side only
 ETHANE 100 283.15 FEI VITROBOT MARK IV
Sample: 3.5 ul; Wait time: 10 s; Blot time: 3 s; Blot force: -2; Drain time: 0 s.
The viral sample was concentrated down to 1x10^10 plaque-forming units per ml (PFU ml^-1)
 FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 30.0 2.7 2.0 2.0 4.0 4.0 42000.0 42000.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN 77.0 77.0 GIF Bioquantum 10 GATAN K3 BIOQUANTUM (6k x 4k) 5760 4092 4323 1.5 2.42 1 I FOURIER SPACE 18.0 FSC 0.143 CUT-OFF RELION 4.0 3152 131 10240 EhV-201 virion vertex reconstruction coming out of localized single particle reconstruction template matching emClarity 1.5.3.11 Warp 1.0.9 MAXIMUM LIKELIHOOD RELION 4.0 emd_17652.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 352 352 352 0 0 0 352 352 352 2196.48 2196.48 2196.48 90.0 90.0 90.0 X Y Z -0.055713035 1.3663783 0.10212083 0.23803337 6.24 6.24 6.24 0.75 AUTHOR emd_17652_additional_1.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 208 208 208 0 0 0 208 208 208 1297.9199 1297.9199 1297.9199 90.0 90.0 90.0 X Y Z -0.7776119 1.3577633 0.05255124 0.19500364 6.24 6.24 6.24 AUTHOR emd_17652_additional_2.map.gz 1 IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES) 208 208 208 0 0 0 208 208 208 1297.9199 1297.9199 1297.9199 90.0 90.0 90.0 X Y Z 0.0 1.0 0.13774274 0.33285433 6.24 6.24 6.24 AUTHOR