2013-01-23 OBS 2007-09-27 PDBe PDBe 2007-09-27 2008-01-18 2013-01-23 EMD-1711
Original map was corrupted
Structure of an archaeal RNA polymerase. Kusser AG, Bertero MG, Naji S, Becker T, Thomm M, Beckmann R, Cramer P Kusser AG, Bertero MG, Naji S, Becker T, Thomm M, Beckmann R, Cramer P Structure of an archaeal RNA polymerase. J.MOL.BIOL. 376 303 307 2008 18164030 doi:10.1016/j.jmb.2007.08.066 emd_1436.map.gz Envelope stored as signed bytes 180 180 180 0 0 0 179 179 179 180 180 180 222.75 222.75 222.75 90 90 90 X Y Z -128 127 -45.8211 15.4496 1
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1.2375 1.2375 1.2375 1 This is an EM density map of a RNA Polymerase from Pyrococcus Furiosus Pyrococcus Furiosus DNA directed RNA Polymerase 1 0.38 0.38 Mass Spectrometry
The sample was monodisperse and catalytically active
protein RNA Polymerase 0.38 0.38 Pyrococcus furiosus false Monomer Archaea 1 Nucleus Nucleus ETHANE 95 115 FEI VITROBOT Blot 10s
Vitrification instrument: FEI Vitrobot
 FEI TECNAI F30 Oxford Instruments OTHER 300 OTHER OTHER FIELD EMISSION GUN 23 O REAL 0.1 Quantifoil R 3/3 grid with 2 nm carbon on top particle
10 mM Hepes pH 7.0, 150 mM KCl, 2.5 mM MgCl2, 5 mM DTT
7.0 singleParticle Reference Projections Signature, SPIDER Each Micrograph 13 FSC 0.5 22240
Particles were selected maually.