REL OBS PDBe PDBe PDBe 2007-09-27 2007-09-27 2008-01-18 2013-01-23 2013-01-23 EMD-1711 Structure of an archaeal RNA polymerase. Kusser AG Bertero MG Naji S Becker T Thomm M Beckmann R Cramer P Kusser AG Bertero MG Naji S Becker T Thomm M Beckmann R Cramer P Structure of an archaeal RNA polymerase. J.MOL.BIOL. 376 303 307 2008 18164030 doi:10.1016/j.jmb.2007.08.066 Pyrococcus Furiosus DNA directed RNA Polymerase Pyrococcus Furiosus DNA directed RNA Polymerase
The sample was monodisperse and catalytically active
1 0.38 0.38 Mass Spectrometry RNA Polymerase Pyrococcus furiosus Archaea Nucleus Nucleus 0.38 0.38 1 Monomer false singleParticle particle 0.1 7.0
10 mM Hepes pH 7.0, 150 mM KCl, 2.5 mM MgCl2, 5 mM DTT
Quantifoil R 3/3 grid with 2 nm carbon on top
 ETHANE 95 115 OTHER
Vitrification instrument: FEI Vitrobot
Blot 10s FEI TECNAI F30 OTHER OTHER FIELD EMISSION GUN 300 OTHER 23 Oxford Instruments
Particles were selected maually.
Each Micrograph
 OTHER 13.0 FSC 0.5 CUT-OFF Signature, SPIDER 22240 emd_1436.map.gz 1 IMAGE STORED AS SIGNED BYTE 180 180 180 0 0 0 180 180 180 222.75 222.75 222.75 90 90 90 X Y Z -128.0 127.0 -45.8211 15.4496 1.2375 1.2375 1.2375 1.0 EMDB This is an EM density map of a RNA Polymerase from Pyrococcus Furiosus
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 O REAL