2022-08-24 OBS PDBe PDBe PDBe 2022-01-21 2022-08-17 2022-08-17 2022-08-24 2022-08-24 Other private Kandolf S Grishkovskaya I Meinhart A Haselbach D Kandolf S Grishkovskaya I Belacic K Foster B Imre R Bolhuis DL Mechtler K Schleiffer A Tagare H Zhong ED Meinhart A Brown NG Haselbach D Plant Communications 3 2022 EMD-14175 associated EM volume EMD-14177 associated EM volume 7qvg FULLOVERLAP Spinach 26S proteasome Spinach 26S proteasome 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 Spinacia oleracea 1.7 26S proteasome regulatory subunit 7 spinach 0.047786055 1 LEVO MAIEHEDDLKDEKNPRPLDEDDIALLKTYGLGPYSASIKKVEKEIKDMSKKVNDLIGIKESDTGLAAPSQWDLVSDKQMM QEEQPLQVARCTKIINPNTEDAKYVINVKQIAKFVVGLGDKVSPTDIEEGMRVGVDRNKYQIQIPLPPKIDPSVTMMTVE EKPDVTYNDVGGCKEQIEKMREVVELPMLHPEKFVKLGIDPPKGVLCYGPPGTGKTLLARAVANRTDACFIRVIGSELVQ KYVGEGARMVRELFQMARSKKACIVFFDEVDAIGGARFDDGVGGDNEVQRTMLEIVNQLDGFDARGNIKVLMATNRPDTL DPALLRPGRLDRKVEFGLPDLEGRTQIFKIHTRTMNCERDIRFELLARLCPNSTGADIRSVCTEAGMYAIRARRKTVTEK DFLDAVNKVIKGYQKFSATPKYMVYN AAA domain-containing protein spinach 0.049635012 1 LEVO MGQNSSGLNRQGGPPGDRKDGEKKEKKYEPAAPPARVGRKQRKQKGSESAARIPTVTPLTKCKLRLLKLERIKDYLLMEE EFVANQERLKPQEEKTEEDRSKVDDIRGSPMSVGSLEELIDENHAIVSSSVGPEYYVSIMSFVDKDQLEPGCSILMHNKV LSVVGLLQDEVDPMVSVMKVEKAPLESYADIGGLDPQIQEIKEAVELPLTHPELYEDIGIKPPKGVILYGEPGTGKTLLA KAVANSTSATFLRVVGSELIQKYLGDGPKLVRELFRVADDLSPSIVFIDEIDAVGTKRYDAHSGGEREIQRTMLELLNQL DGFDSRGDVKVILATNKIESLDPALLRPGRIDRKIEFPLPDIKTRRRIFTIHTSKMTLSDDVNLEEFVMTKDEFSGADIK AICTEAGLLALRERRMKVTHTDFKKAKEKVMFKKKEGVPEGLYM AAA domain-containing protein spinach 0.04581016 1 LEVO MATMEADQKTVKQGEGLRQYYLQHIHELQLRVRNKNHNLQRLEAQRNDLNSHVRALKEELQLLQEPGSYVGEVVKVMGKS KVLVKVHPEGKYVVDIDKNIDITKLTPTTRVALRNDSYVLHLVLPSKVDPLVNLMKVEKVPDSTYDMIGGLDQQIKEIKE VIELPIKHPELFESLGIAQPKGVLLYGPPGTGKTLLARAVAHHTDCTFIRVSGSELVQKYIGEGSRMVRELFVMAREHAP SIIFMDEIDSIGSTRMESGSGNGDSEVQRTMLELLNQLDGFEASNKIKVLMATNRIDILDPALLRPGRIDRKIEFPNPTE ESRFDILKIHSRRMNLMRGIDLKKIGDKMNGASGAELKSVCTEAGMFALRERRIHVTQEDFEMAVAKVMKKDTDKNMSLR KLWK AAA domain-containing protein spinach 0.047132694999999995 1 LEVO MAASAMVLDPKPVPPPPQPLEPPATVSFTASEDTSVVEEEDLYGRLKSLERQIEFKGIQEEYVKDELKNLKREHLRAQEE VKRIQSVPLVIGQFMEMVDQNNGIVGSTTGSNYYVRILSTINRELLKPSASVALHRHSNALVDVLPPEADSSISLLSQSE KPDVSYNDIGGCDIQKQEIREAVELPLTHHDLYKQIGIDPPRGVLLYGPPGTGKTMLAKAVANHTTAAFIRVVGSEFVQK YLGEGPRMVRDVFRLAKENAPAIIFIDEVDAIATARFDAQTGADREVQRILMELLNQMDGFDQTVNVKVIMATNRADTLD PALLRPGRLDRKIEFPLPDRRQKRLVFQVCTAKMNLSDEVDLEDYVSRPDKISAAEITAICQEAGMHAVRKNRYVILPKD FEKGYRSNVKKPDTDFDFYK AAA domain-containing protein spinach 0.044596266 1 LEVO MATEEDVKRRAATSEYNKKLLQHRELESRSRKVKEELRSAKKDYTKTEDDLKSLQSVGQIIGEVLRPLDDERLIVKASSG PRYVVGCRSKVDKEKLTSGTRVVLDMTTLTIMRALPREVDPVVYNMLHEDPGNISYSAVGGLSDQIRELRESIELPLMNP ELFIRVGIKPPKGVLLYGPPGTGKTLLARAIASNIDANFLKVVSSAIIDKYIGESARLIREMFNYAREHQPCIIFMDEID AIGGRRFSEGTSADREIQRTLMELLNQLDGFDQLGKVKMIMATNRPDVLDPALLRPGRLDRKIEIPLPNEQSRMEILKIH GAGIAKHGEIDYEAVVKLAEGFNGADLRNICTEAGMSAIRAERDYVIHEDFMKAVRKLNEAKKLESSATYSADFGKD AAA domain-containing protein spinach 0.047754305 1 LEVO MASASATAMVEDPNFQDDQLANLTTEDIARASRILDNEIRILKEDVQRTTLELDSFKEKIKENQEKIKLNKQLPYLVGNI VEILEMNPEDEAEEDGANIDLDSQRKGKCVVLKTSTRQTIFLPVVGLVDPDKLKPGDLVGVNKDSYLILDTLPSEYDSRV KAMEVDEKPTEDYSDIGGLEKQIQELVEAIVLPMTHKERFQTIGIRPPKGVLLYGPPGTGKTLMARACAAQTNATFLKLA GPQLVQMFIGDGAKLVRDAFQLAKEKAPCIIFIDEIDAIGTKRFDSEVSGDREVQRTMLELLNQLDGFSSDERIKVIAAT NRADILDPALMRSGRLDRKIEFPHPTEEARARILQIHSRKMNVHPDVNFEELARSTDDFNGAQLKAVCVEAGMLALRRDA TEVNHEDFNEGIIQVQAKKKASLNYYA 26S proteasome regulatory subunit RPN11 spinach 0.034506883 1 LEVO MDRLTRMMAGAGGALGHPPPDSPTLDTSEQVYISSLALLKMLKHGRAGVPMEVMGLMLGEFVDEYTVKVVDVFAMPQSGT GVSVEAVDPVFQTNMLDMLKQIGRPEMVVGWYHSHPGFGCWLSGVDINTQQSFEALNQRAVAVVVDPIQSVKGKVVMDAF RLINPQTMMLGQEPRQTTSNLGHLNKPSIQALIHGLNRHYYSIAINYRKNELEEKMLLNLHKKKWNDGLTLKKFDVHSKT NEQTVQEMLGLAIKYNKAVQEEDELTPEKLVIAKVGRQDAKKHLEEHVSNLMSSNIIQTLGTMLDTVIF PCI domain-containing protein spinach 0.055356188 1 LEVO MTQDVEMHDTQPLSISLSSVDPPTLKHLKEIASLLETGSYTKEARRISRAIRLTFAVRRKLTARVLHFFLDYALTPGSEA HGKISAFLPKGGEHEMEVDTATSAVQPSGKSTLPELEIFCYLIVLLFLIDQKLHNEAKACASASIARLKSLKRRVADVLA SKLYSFYSLSYELTGDLAEIRGELLTLHRLTTLHHDELGQETLLNLLLRNYLHYNLYDQAEKLRSKAPSFGAHSNQQHCR HLFYVGKIQTIQLEYTDAKESLLQAARKAPIAARGFRIQCNKWAVIVRLLLGEIPERTMFMQKGMEKALRPYFELTNAVR IGDLELFRGVTEKFSSTFDKDRTHNLIVRLRHNVIRTGLRNISISYSRISLSDVAKKLRLDSPNPVADAESIVAKAIRDG AIDATLDHGNGWMLSKETGDIYSTTEPQSAFDSRIAFCLNMHNEAVRALRYPPNSHKEKESAEKRRERQQQEQELAKHIA EEEDDDF PCI domain-containing protein spinach 0.030804094 1 LEVO MDPKLTEIAQTFERFKAASVRNDFDTCTRLLTQLKVSLIEYRSLPPMFEATPNAVHELTLARDIYEHAVVLSVKMEDQEA FERDFCQLKPYYTDARGRIPQSTQEYPILGLNLLRLLVQNRIAEFHTELELLSSTALENPCIKHAVELEQSFMEGAYNRV LSARQTVPHETYVHFMDLLAKTVRDEIAGCSEKAYDTLSVNDARQLLLFSSDKDLLEYIKEEHPEWEIKDGCVVFQKSKD SATCKEIPSLLLINQTLSYARELERIV VWFA domain-containing protein spinach 0.042562171999999995 1 LEVO MVLEATMICIDNSEWMRNGDYSPNRFQALSDAVNLICGAKTQSNPENTVGILTMAGKGVRVLVTPTSDLGKILACMHGLD IGGEMNLAAGIQVAQLALKHRQNKKQQQRIIVFAGSPVNYDKKVLEMIGRKLKKNSVALDVVDFGEDEEGKSEKLEALVA AVNNNETSHIVHVPPGGIALSDVLISTPIFTGDGEGGSGFAAAAAAAAAGGVSGYDFGVDPNLDPELALALRVSMEEERA RQEAAAKRAAEEATGQEKGGEQSSQDATMGESSNAAISGADKKNDMTEDDENDLLQQALAMSMDDPVSTTIVQDTDMADA AGDDQDLQLALQLSVEEGAKESTSQSSQTDMSKLLADQSFVSSILASLPGVDPNDPSVKDLLASMHSQSEKKDEDKPPSE EEK PCI domain-containing protein spinach 0.04424283999999999 1 LEVO MSALQYLDTLRSAHPELGEWYNTLADLYQKKLWHQLTLELEKFVALAVFQAGDALIQLYHNFITDFETKINLLKLAHFAV IVSRQYAEKEAAIGYLEGVTEKLHATKENRIEEPVLYIKMQIALFKLEQGEQKECKKLLDNGKTTLDSMTDIDPSVYATF YWVSSQYHKARQEFAEFYKNALLYLAYTSVESLSESFKLDLAFDLSLSALLGENIYNFGELLAHPILKSLLGTKVEWLYY ILQAFNTGDLIRYQELCNVHKDALNAQPALVANERKLLEKINILCLMEIIFNRPAEDRTIPLKVIAERTRLSIEDVEYLL MKSLSVHLIEGIIDQMEGTVHVSWVQPRVLGIPQITSLRDRLDSWLGKVNTALLSVEAETPDLVAS MPN domain-containing protein spinach 0.034457531 1 LEVO QIFSSKSIEKVVVHPLVLLSIVDHYNRVARDTKKRVIGVLLGSTFKGTVDVTNSYAVPFEEDDKDSSIWFLDHNYHESMF SMFRRINAKEHVVGWYSTGPKLRENDLDVHRLFSDYVPNPVLVIIDVQPEELGIPTKAYYAVEEVKENATQKSQKVFVHV PSEIAAHEVEEIGVEHLLRDVKDTTISTLATEVTGKLGALKGLDARLREIRSYLELVIQEKLPLNHEILYHLQDVFNLLP NLSVLELVKAFAVKTNDMMLVIYLSSLIRSVIALHNLINNKMLNKEHEKAEDSKSLAITSVAGS PCI domain-containing protein spinach 0.047236547 1 LEVO MATFLPATTDSLAQALEASSTADSILILQRLLADPSSSPDALRIKEQAITKLTDYLRQENKAEDLRILLTQLRSYFSLIP KAKTAKIVRVIIDTVAKIPNSTELQISLCKDMIQWTRDEKRTFLRQRVEARLAALLMETKEFPEALSLLSGLIKEVRRLD DKLLLVDIELLESKLHFSLRNLPKAKAALTAARTAANAIYVPPAQQGTIDLQSGILHAEEKDYKTGYSYFYEAFEAFNAL EDPRAVYSLKYMLLCKVMVNQADDVAGIISSKAGLQYLGPDLDAMKAIADAYSKRSLKLFEASLVNFKAQLQEDPIIHRH LSSLYDTLLEQNLCRLIEPFSKVEISHIAELIELPVDHVERKLSQMILDKKFAGTLDQGAGCLIIFDDHKTEDIYEATLD TISNVAKVVDSLFVRSAKIMA PCI domain-containing protein spinach 0.050790062 1 LEVO MDNGGNLEAQIDALLNVEKQMRLAGDVAGTRKAACDILDLCFQSKAWKTLNDQIVVLSKRRGQLKQAVTAMVQQAMGYID QTPDLDIRVELIKTLNSVSAGKIYVELERARLIKILAKIKEQQGLIDEAAELMQEIAVETFGAMAKTEKIAFILEQVRLC LDRKDYIRAQILSRKISPRVFDIDPSKEKKKPKEGDNIVEEAPADIPSLPELKRIYYELMIRYYKHHNDYLEICRCYKSI YEISSVKEDPEQWTPILRKICWYLALAPHDPMQSSLLNSTLEDKNLFEIPKFKSLLKQLVTMEVILWTVLWNEFESEFDN EKNLLGGPLGEKAGEDLKQRVIEHNILVISKYYSRITLKRLSDLLCLSLQEAEKHLSDMVVSKALIAKIDRPMGIVCFQV VKDSNDILNSWSMNLEKLLDLVEKSCHQIHKETMVHKASLEV 26S proteasome non-ATPase regulatory subunit 2 homolog spinach 0.098484406 1 LEVO MDPEPSNTGAGNPTENRKEATTKVPSKDPKKKDDKKDEDLSEEDLALKQQLELYVERVQDPDAGLQRVALESMRQEIRSA TSSMTSVPKPLKFLRPHYGTLKAFYETMSDSDLKEFLADILSVLALTMSAEGERESLKYRLLGSEGDIGSWGHEYVRNLA GEIAQEYSKQHQNEEAPFDDLMDLVQQIIAFHMKHNAEPEAVDLLMEVEDLDLLQEHVDEKNYKRTCLYLTAAAKYLASP DDMLVLDIAHSIYMKCEEYPSALQVSLVLDNLQYVKHIFTSCNDLLRKKQFCYILARHGVLFELDDNMVLDDDEREQMQD IINNYKLSEGYLTLARDIEVMEAKSPEDIYKAHLLDGRASAGATVDSARQNLAATFVNAFVNAGFGQDKLMTGPADSSSG SSSGNWLFKNKEHGKTSAAASLGMILLWDVDSGLAQLDKYFHSTDNHVIAGALLGVGIVNCGVKNDCDPALALLSDYVDK EDPAIRIGAIMGLGISYANTQNEQLRSKLTPILGDTNASLDVIAFTAISLGLVFVGSCNEEVAQAIIFALMDRSEADLGE PLARLLPLGLGLLYLGKQESVEATAEVSKTFNEKIRKHCDMTLLSCAYAGTGNVLKVQSLLGHCAQHLDKGETHQGPAVL GIAMVAMAEELGVEMAIRSLEHLLQYGEQNIRRAVPLALGLLCISNPKVNVMDTLSRLSHDSDLEVAMAAIISLGLIGAG TNNARIAGMLRNLSSYYYKDASALFCVRIAQGLVHMGKGLLTLNPYHSDRFLLSPSAHAGLVIMLHACLDMKAIILGKYH YMLYFLVLAMQPRMLLTVDENLKPLSVPVRVGQAVDVVGQAGRPKTITGFQTHSTPVLLSAGDRAELATEKYIPLSSILE GFVILKENPEYREEH 26S proteasome non-ATPase regulatory subunit 1 homolog spinach 0.10919921099999999 1 LEVO MANLATSAGGMLAMLSEHSTSLKLHALSNLNVYAQFLWPEISTSIPLLESLYEDEEFSQRPLAALVVSKVFYFLGELNDS LAYALGAGSLFDVSEDSDYVRTLLDKAIDEYASLRNKSAESKEEAVNIDPRLEAIVERMLEKCILDGRYQQAMGMAIECR RLDKLEEAIMRSDNAPGSLAYCINVSHSYVNRREYRQEVLRLLVRVYQKLPSPDYLSICQCLMFLDQPEAVASILEKLLR AEKLEDTLLSFQIAFDLVENEHQAFLLNVRDRLSKPKSSSQLPISTEPESSQTENTTASEDVQMSDETRVSDAVVGEMDP TEARYAERLTKIKGILSGETSIQLTLQFLYSHNKSDLLILKTIKQSVEMRNSVCHSATIYANAIMHAGTTVDTFLRENLD WLSRATNWAKFSATAGLGVIHRGHLQQGRSLMAPYLPQGGAGGGGSPYSEGGALYALGLIHANHGEGIKQFLRDSLRSTN VEVIQHGACLGLGLAALGTADEDVFEDIKNVLYTDSAVAGEAAGISMGLLMVGTASEKAGEMLAYAHETQHEKIIRGLAL GIALTVYGREEEADTLIEQMTRDQDPILRYGGMYALALAYRGTSNNKAIRQLLHFAVSDVSDDVRRTAVLALGFVLYSEP EQTPRIVSLLSESYNPHVRYGAALAVGISCAGTGLSEAISLLEPLTSDVVDFVRQGALIAMAMVMVQITEAMDSRVGTFR RQLEKIILDKHEDTMSKMGAILASGILDAGGRNVTIRLLSKSKHDKITAVVGLAVFSQFWYWYPLIYFISLAFSPTAFVG LNYDLKVPKFDFLSHAKPSLFEYPKPTTVPTTASAVKLPTAVLSTSVKAKARAKKEAEQKDKSVGSEPSASTSASGKGKS STEKEGDSMLVDSLPEKKAEAEPCFETITNPARVVPAQEKFIKFLEGSRYMPVKLAASGFVLLKDLRPEEPEVLSLTDAP ASASATTNSAAVAAPVQQGTVAAMAVDEEPQPPQPFEYTS PCI domain-containing protein spinach 0.044432875 1 LEVO MEAQEGSQNEHLKLANKIFHLTHPDVEDIEKVSLKEEVLSAIKSDFMVSLYETLAGNGVLELDQALLDSMRQSIEDELKK LDEKIADAEENLGESEVREAHLAKSLFYIRIGDKDKALEQLKVTETKTVAVGQKMDLVFFTLQVGLFDMDFDLISRSIDK AKNLFEEGGDWERKNRLKVYEGLYCMSTRDFKKAASLFLDSISTFTTYELFPYDTFIFYTVLTSIISLDRVSLKQKVVDA PEILTVIGKIPYLSEFLNSLYDCQYKSFFSAFAGLTEQIKFDRYLHRHFRYYMREVRTVVYSQFLESYKSVTIEAMAKAF GVTVEFIDLELSRFIAAGKLHCKIDKVVGVLETNRPDAKNALYQATIKQGDFLLNRIQKLSRVIDL ADENOSINE-5'-TRIPHOSPHATE 0.000507181 1 ATP ADENOSINE-5'-DIPHOSPHATE 0.000427201 1 ADP singleParticle particle 0.02 6.5 25.0 Bis-Tris bis(2-hydroxyethyl)amino-tris(hydroxymethyl)methan 50.0 KCl potassium chloride 5.0 MgCl2 magnedium chloride 5.0 glycerol glycerol 20.0 ATP adenosine triphosphate 5.0 DTT dithiothreitol 5.0 PVP polyvinylpolypyrrolidone 2.0 PMSF phenylmethylsulfonyl fluoride Quantifoil R3.5/1 COPPER 200 CARBON CONTINUOUS 2.0 GLOW DISCHARGE AIR ETHANE 80 277 LEICA EM GP FEI TITAN KRIOS FLOOD BEAM BRIGHT FIELD FIELD EMISSION GUN 300 70.0 2.7 1.0 4.0 FEI TITAN KRIOS AUTOGRID HOLDER NITROGEN FEI FALCON III (4k x 4k) INTEGRATING 24769 80.0 FEI FALCON III (4k x 4k) INTEGRATING 8089 50.0 1 951422 cryoSPARC 3.0 C1 FOURIER SPACE 3.3 FSC 0.143 CUT-OFF cryoSPARC 3.0 951422 OTHER cryoSPARC 3.0 PROJECTION MATCHING cryoSPARC 3.0 cryoSPARC 3.0 6MSB RIGID BODY FIT REAL 109.9